RCEM_CERS4
ID RCEM_CERS4 Reviewed; 308 AA.
AC Q3J1A6; P02953; Q9RFB8;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Reaction center protein M chain;
DE AltName: Full=Photosynthetic reaction center M subunit;
GN Name=pufM; OrderedLocusNames=RHOS4_18600; ORFNames=RSP_0256;
OS Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
OS 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter
OS sphaeroides).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Cereibacter.
OX NCBI_TaxID=272943;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10648776; DOI=10.1093/nar/28.4.862;
RA Choudhary M., Kaplan S.;
RT "DNA sequence analysis of the photosynthesis region of Rhodobacter
RT sphaeroides 2.4.1.";
RL Nucleic Acids Res. 28:862-867(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203
RC / NCIMB 8253 / ATH 2.4.1.;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C.,
RA Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.;
RT "Complete sequence of chromosome 1 of Rhodobacter sphaeroides 2.4.1.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The reaction center is a membrane-bound complex that mediates
CC the initial photochemical event in the electron transfer process of
CC photosynthesis. {ECO:0000250}.
CC -!- SUBUNIT: Reaction center is composed of four bacteriochlorophylls, two
CC bacteriopheophytins, two ubiquinones, one iron, and three highly
CC hydrophobic polypeptide chains (designated L, M, and H). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cellular chromatophore membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the reaction center PufL/M/PsbA/D family.
CC {ECO:0000305}.
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DR EMBL; AF195122; AAF24305.1; -; Genomic_DNA.
DR EMBL; CP000143; ABA79428.1; -; Genomic_DNA.
DR PIR; T50761; T50761.
DR RefSeq; WP_002720420.1; NZ_CP030271.1.
DR RefSeq; YP_353329.1; NC_007493.2.
DR PDB; 2WX5; X-ray; 2.63 A; M=2-308.
DR PDB; 4IN5; X-ray; 2.20 A; M=1-307.
DR PDB; 4IN6; X-ray; 2.70 A; M=2-303.
DR PDB; 4N7L; X-ray; 2.85 A; M=2-304.
DR PDB; 5LRI; X-ray; 2.40 A; M=2-308.
DR PDB; 7PIL; EM; 2.50 A; M=2-308.
DR PDB; 7PQD; EM; 2.90 A; M/m=2-308.
DR PDBsum; 2WX5; -.
DR PDBsum; 4IN5; -.
DR PDBsum; 4IN6; -.
DR PDBsum; 4N7L; -.
DR PDBsum; 5LRI; -.
DR PDBsum; 7PIL; -.
DR PDBsum; 7PQD; -.
DR AlphaFoldDB; Q3J1A6; -.
DR SMR; Q3J1A6; -.
DR STRING; 272943.RSP_0256; -.
DR DrugBank; DB04147; Dodecyldimethylamine N-oxide.
DR EnsemblBacteria; ABA79428; ABA79428; RSP_0256.
DR GeneID; 57470574; -.
DR GeneID; 67446989; -.
DR KEGG; rsp:RSP_0256; -.
DR PATRIC; fig|272943.9.peg.2199; -.
DR eggNOG; ENOG502Z87P; Bacteria.
DR OMA; IFPHLDW; -.
DR PhylomeDB; Q3J1A6; -.
DR Proteomes; UP000002703; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030077; C:plasma membrane light-harvesting complex; IEA:InterPro.
DR GO; GO:0042717; C:plasma membrane-derived chromatophore membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042314; F:bacteriochlorophyll binding; IEA:UniProtKB-KW.
DR GO; GO:0045156; F:electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009772; P:photosynthetic electron transport in photosystem II; IEA:InterPro.
DR CDD; cd09291; Photo-RC_M; 1.
DR Gene3D; 1.20.85.10; -; 2.
DR InterPro; IPR036854; Photo_II_D1/D2_sf.
DR InterPro; IPR000484; Photo_RC_L/M.
DR InterPro; IPR005781; Photo_RC_M.
DR Pfam; PF00124; Photo_RC; 1.
DR PRINTS; PR00256; REACTNCENTRE.
DR SUPFAM; SSF81483; SSF81483; 1.
DR TIGRFAMs; TIGR01115; pufM; 1.
DR PROSITE; PS00244; REACTION_CENTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Bacteriochlorophyll; Chlorophyll; Chromophore;
KW Electron transport; Iron; Magnesium; Membrane; Metal-binding;
KW Photosynthesis; Reaction center; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..308
FT /note="Reaction center protein M chain"
FT /id="PRO_0000090418"
FT TRANSMEM 54..80
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TRANSMEM 111..140
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TRANSMEM 143..168
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TRANSMEM 198..226
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TRANSMEM 260..286
FT /note="Helical"
FT /evidence="ECO:0000250"
FT BINDING 183
FT /ligand="(7R,8Z)-bacteriochlorophyll b"
FT /ligand_id="ChEBI:CHEBI:30034"
FT /ligand_part="Mg"
FT /ligand_part_id="ChEBI:CHEBI:25107"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT BINDING 203
FT /ligand="(7R,8Z)-bacteriochlorophyll b"
FT /ligand_id="ChEBI:CHEBI:30034"
FT /ligand_part="Mg"
FT /ligand_part_id="ChEBI:CHEBI:25107"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT BINDING 220
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT BINDING 235
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT BINDING 253
FT /ligand="a ubiquinone"
FT /ligand_id="ChEBI:CHEBI:16389"
FT /evidence="ECO:0000250"
FT BINDING 267
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT CONFLICT 245
FT /note="A -> E (in Ref. 1; AAF24305)"
FT /evidence="ECO:0000305"
FT STRAND 6..8
FT /evidence="ECO:0007829|PDB:4IN5"
FT STRAND 12..14
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 27..29
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 38..41
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 55..78
FT /evidence="ECO:0007829|PDB:4IN5"
FT TURN 79..81
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 83..88
FT /evidence="ECO:0007829|PDB:4IN5"
FT TURN 89..92
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 100..102
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 110..112
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 114..140
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 146..162
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 164..169
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 172..174
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 180..193
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 197..199
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 201..226
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 228..230
FT /evidence="ECO:0007829|PDB:4IN5"
FT TURN 231..233
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 235..240
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 244..257
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 265..287
FT /evidence="ECO:0007829|PDB:4IN5"
FT TURN 289..291
FT /evidence="ECO:0007829|PDB:4IN5"
FT HELIX 295..300
FT /evidence="ECO:0007829|PDB:4IN5"
SQ SEQUENCE 308 AA; 34509 MW; 30B3737DF8658250 CRC64;
MAEYQNIFSQ VQVRGPADLG MTEDVNLANR SGVGPFSTLL GWFGNAQLGP IYLGSLGVLS
LFSGLMWFFT IGIWFWYQAG WNPAVFLRDL FFFSLEPPAP EYGLSFAAPL KEGGLWLIAS
FFMFVAVWSW WGRTYLRAQA LGMGKHTAWA FLSAIWLWMV LGFIRPILMG SWSEAVPYGI
FSHLDWTNNF SLVHGNLFYN PFHGLSIAFL YGSALLFAMH GATILAVSRF GGERELEQIA
DRGTAAERAA LFWRWTMGFN ATMEGIHRWA IWMAVLVTLT GGIGILLSGT VVDNWYVWGQ
NHGMAPLN
