RCH1_CANAL
ID RCH1_CANAL Reviewed; 411 AA.
AC Q59UQ7;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Solute carrier RCH1 {ECO:0000303|PubMed:22530691};
DE AltName: Full=Regulator of calcium homeostasis 1 {ECO:0000303|PubMed:22530691};
GN Name=RCH1 {ECO:0000303|PubMed:22530691};
GN OrderedLocusNames=CAALFM_C400360CA; ORFNames=orf19.5663;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=22530691; DOI=10.1042/bj20112166;
RA Jiang L., Alber J., Wang J., Du W., Yang X., Li X., Sanglard D., Geyer J.;
RT "The Candida albicans plasma membrane protein Rch1p, a member of the
RT vertebrate SLC10 carrier family, is a novel regulator of cytosolic Ca2+
RT homoeostasis.";
RL Biochem. J. 444:497-502(2012).
CC -!- FUNCTION: Solute carrier protein that negatively regulates the
CC cytosolic homeostasis in response to high levels of extracellular
CC calcium. {ECO:0000269|PubMed:22530691}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22530691};
CC Multi-pass membrane protein {ECO:0000269|PubMed:22530691}. Bud neck
CC {ECO:0000269|PubMed:22530691}. Note=Distributes as multiple foci in the
CC plasma membrane prior to cell division, moves toward and concentrates
CC at the bud neck as the bud grows in size, and disperses again along the
CC plasma membrane immediately prior to cytokinesis.
CC {ECO:0000250|UniProtKB:Q05131}.
CC -!- DISRUPTION PHENOTYPE: Leads to modest tolerance to azoles and
CC terbinafine, but not to amphotericin B and nystatin (PubMed:22530691).
CC Elevates the intracellular Ca(2+) and leads to the activation of the
CC calcium/calcineurin signaling pathway (PubMed:22530691).
CC {ECO:0000269|PubMed:22530691}.
CC -!- SIMILARITY: Belongs to the bile acid:sodium symporter (BASS) (TC
CC 2.A.28) family. {ECO:0000305}.
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DR EMBL; CP017626; AOW28829.1; -; Genomic_DNA.
DR RefSeq; XP_713344.1; XM_708251.1.
DR AlphaFoldDB; Q59UQ7; -.
DR STRING; 237561.Q59UQ7; -.
DR TCDB; 2.A.28.3.7; the bile acid:na(+) symporter (bass) family.
DR PRIDE; Q59UQ7; -.
DR EnsemblFungi; KHC77987; KHC77987; W5Q_03337.
DR EnsemblFungi; KHC86838; KHC86838; I503_03338.
DR GeneID; 3645002; -.
DR KEGG; cal:CAALFM_C400360CA; -.
DR CGD; CAL0000187136; RCH1.
DR VEuPathDB; FungiDB:C4_00360C_A; -.
DR eggNOG; KOG4821; Eukaryota.
DR HOGENOM; CLU_039013_3_0_1; -.
DR InParanoid; Q59UQ7; -.
DR OMA; FFFYGLK; -.
DR OrthoDB; 949808at2759; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0005935; C:cellular bud neck; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:CGD.
DR GO; GO:0070509; P:calcium ion import; IMP:CGD.
DR GO; GO:0051481; P:negative regulation of cytosolic calcium ion concentration; IEA:EnsemblFungi.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR016833; Put_Na-Bile_cotransptr.
DR PANTHER; PTHR18640; PTHR18640; 1.
DR Pfam; PF13593; SBF_like; 1.
DR PIRSF; PIRSF026166; UCP026166; 1.
PE 3: Inferred from homology;
KW Calcium; Calcium transport; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..411
FT /note="Solute carrier RCH1"
FT /id="PRO_0000448612"
FT TOPO_DOM 1..17
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 39..52
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..89
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..120
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..150
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..204
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..242
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..269
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 291..329
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 351..361
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 383..411
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
SQ SEQUENCE 411 AA; 46095 MW; 28155A6404D7D9EC CRC64;
MSLETFRESK AYKWTSKVIS FLIGQWFFIF LGVFIALAHS YPEFAKQGGT IRAEYSIGYG
AVAVIFLISG LSMSTKQLLV NVANWRAHFT VLSMSFLVTS AIIYGIASGI KASHNGQIDD
WLLIGLIVTH ACPTTVSSNV VMTKQAHGND ILTLCEVFIG NVLGAFITPA LLQMYMRGTW
EIGNPSHQTQ GDSTVQELYA HTMKQLGLSV FVPLFVGQVV QNIFPKQTKW CLTTFKLNKV
GSFMLLLIMF QSFSTAFAQH AFTSVSHASI IFLVFFNIGI YLFFTVLTFF YSRPFWILRV
FKEEPNESSS KLYRYSYAFF RPFYYNRKDT VAVMLCGPAK TAALGVSLVS SQYGSHNPKL
GIILVPLVLY QAEQVMTANV LVSFMRKWIH AEDKVPEDEE TSVGSDNDPK K
