ID   RCI1_ECOLX              Reviewed;         384 AA.
AC   P10487;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Shufflon-specific DNA recombinase;
GN   Name=rci;
OS   Escherichia coli.
OG   Plasmid IncI1 R64.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3065610; DOI=10.1007/bf00333394;
RA   Kubo A., Kusukawa A., Komano T.;
RT   "Nucleotide sequence of the rci gene encoding shufflon-specific DNA
RT   recombinase in the IncI1 plasmid R64: homology to the site-specific
RT   recombinases of integrase family.";
RL   Mol. Gen. Genet. 213:30-35(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
RX   PubMed=3029698; DOI=10.1093/nar/15.3.1165;
RA   Komano T., Kubo A., Nisioka T.;
RT   "Shufflon: multi-inversion of four contiguous DNA segments of plasmid R64
RT   creates seven different open reading frames.";
RL   Nucleic Acids Res. 15:1165-1172(1987).
CC   -!- FUNCTION: Shufflon-specific DNA recombinase.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; AB027308; BAA77989.1; -; Genomic_DNA.
DR   PIR; S03815; S03815.
DR   RefSeq; WP_001139957.1; NZ_WVVP01000033.1.
DR   RefSeq; YP_006954452.1; NC_019097.1.
DR   RefSeq; YP_008998813.1; NC_023329.1.
DR   RefSeq; YP_009061175.1; NC_024975.1.
DR   RefSeq; YP_009061389.1; NC_024977.1.
DR   RefSeq; YP_009061640.1; NC_024979.1.
DR   RefSeq; YP_009072185.1; NC_025198.1.
DR   AlphaFoldDB; P10487; -.
DR   SMR; P10487; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR035069; TTHA1013/TTHA0281-like.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF143100; SSF143100; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid.
FT   CHAIN           1..384
FT                   /note="Shufflon-specific DNA recombinase"
FT                   /id="PRO_0000197535"
FT   DOMAIN          9..96
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          118..284
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        155
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        180
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        235
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        238
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        262
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        271
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   384 AA;  44254 MW;  3F9C352498D50516 CRC64;
     MPSPRIRKMS LSRALDKYLK TVSVHKKGHQ QEFYRSNVIK RYPIALRNMD EITTVDIATY
     RDVRLAEINP RTGKPITGNT VRLELALLSS LFNIARVEWG TCRTNPVELV RKPKVSSGRD
     RRLTSSEERR LSRYFREKNL MLYVIFHLAL ETAMRQGEIL ALRWEHIDLR HGVAHLPETK
     NGHSRDVPLS RRARNFLQMM PVNLHGNVFD YTASGFKNAW RIATQRLRIE DLHFHDLRHE
     AISRFFELGS LNVMEIAAIS GHRSMNMLKR YTHLRAWQLV SKLDARRRQT QKVAAWFVPY
     PAHITTIDEE NGQKAHRIEI GDFDNLHVTA TTKEEAVHRA SEVLLRTLAI AAQKGERVPS
     PGALPVNDPD YIMICPLNPG STPL