RCLR_ECOLI
ID RCLR_ECOLI Reviewed; 284 AA.
AC P77379; Q2MCB7;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=RCS-specific HTH-type transcriptional activator RclR;
DE AltName: Full=Reactive chlorine resistance protein R;
GN Name=rclR; Synonyms=ykgD; OrderedLocusNames=b0305, JW0298;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, DNA-BINDING, ACTIVITY REGULATION, DISULFIDE BOND, INDUCTION,
RP DISRUPTION PHENOTYPE, GENE NAME, AND MUTAGENESIS OF CYS-21; HIS-42; HIS-75
RP AND CYS-89.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=24078635; DOI=10.1074/jbc.m113.503516;
RA Parker B.W., Schwessinger E.A., Jakob U., Gray M.J.;
RT "The RclR protein is a reactive chlorine-specific transcription factor in
RT Escherichia coli.";
RL J. Biol. Chem. 288:32574-32584(2013).
CC -!- FUNCTION: Involved in reactive chlorine species (RCS) stress
CC resistance. Up-regulates, in response to hypochlorous acid (HOCl), the
CC expression of three genes essential for survival of RCS stress (rclA,
CC rclB and rclC) and its own expression. {ECO:0000269|PubMed:24078635}.
CC -!- ACTIVITY REGULATION: Oxydation of Cys-21 leads to partial activation of
CC RclR, followed by the formation of an intramolecular disulfide bond
CC between Cys-21 and Cys-89, which stabilizes the active form of RclR.
CC {ECO:0000305|PubMed:24078635}.
CC -!- INDUCTION: Autoregulated. Induced in response to HOCl.
CC {ECO:0000269|PubMed:24078635}.
CC -!- DISRUPTION PHENOTYPE: Mutants are more sensitive to HOCl treatment than
CC wild-type cells. {ECO:0000269|PubMed:24078635}.
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DR EMBL; U73857; AAB18032.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73408.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76089.1; -; Genomic_DNA.
DR PIR; A64757; A64757.
DR RefSeq; NP_414839.1; NC_000913.3.
DR RefSeq; WP_000339594.1; NZ_SSZK01000067.1.
DR AlphaFoldDB; P77379; -.
DR SMR; P77379; -.
DR BioGRID; 4262810; 9.
DR IntAct; P77379; 5.
DR STRING; 511145.b0305; -.
DR PaxDb; P77379; -.
DR PRIDE; P77379; -.
DR EnsemblBacteria; AAC73408; AAC73408; b0305.
DR EnsemblBacteria; BAE76089; BAE76089; BAE76089.
DR GeneID; 945616; -.
DR KEGG; ecj:JW0298; -.
DR KEGG; eco:b0305; -.
DR PATRIC; fig|511145.12.peg.312; -.
DR EchoBASE; EB3351; -.
DR eggNOG; COG2207; Bacteria.
DR HOGENOM; CLU_000445_81_0_6; -.
DR InParanoid; P77379; -.
DR OMA; CMDFELG; -.
DR PhylomeDB; P77379; -.
DR BioCyc; EcoCyc:G6175-MON; -.
DR PRO; PR:P77379; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IDA:EcoCyc.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEP:EcoCyc.
DR GO; GO:0090347; P:regulation of cellular organohalogen metabolic process; IEP:EcoCyc.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR032783; AraC_lig.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF12852; Cupin_6; 1.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 1: Evidence at protein level;
KW Activator; Disulfide bond; DNA-binding; Reference proteome;
KW Stress response; Transcription; Transcription regulation.
FT CHAIN 1..284
FT /note="RCS-specific HTH-type transcriptional activator
FT RclR"
FT /id="PRO_0000194613"
FT DOMAIN 177..278
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 197..218
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 245..268
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DISULFID 21..89
FT /evidence="ECO:0000305|PubMed:24078635"
FT MUTAGEN 21
FT /note="C->A: Does not induce expression of target genes in
FT response to HOCl."
FT /evidence="ECO:0000269|PubMed:24078635"
FT MUTAGEN 42
FT /note="H->A: Increases expression of the target genes in
FT the absence of HOCl."
FT /evidence="ECO:0000269|PubMed:24078635"
FT MUTAGEN 75
FT /note="H->A: Increases expression of the target genes in
FT the absence of HOCl. In vitro, increases DNA binding under
FT reducing conditions."
FT /evidence="ECO:0000269|PubMed:24078635"
FT MUTAGEN 89
FT /note="C->A: Does not induce expression of target genes in
FT response to HOCl."
FT /evidence="ECO:0000269|PubMed:24078635"
SQ SEQUENCE 284 AA; 31474 MW; 1896705347276040 CRC64;
MDALSRLLML NAPQGTIDKN CVLGSDWQLP HGAGELSVIR WHALTQGAAK LEMPTGEIFT
LRPGNVVLLP QNSAHRLSHV DNESTCIVCG TLRLQHSARY FLTSLPETLF LAPVNHSVEY
NWLREAIPFL QQESRSAMPG VDALCSQICA TFFTLAVREW IAQVNTEKNI LSLLLHPRLG
AVIQQMLEMP GHAWTVESLA SIAHMSRASF AQLFRDVSGT TPLAVLTKLR LQIAAQMFSR
ETLPVVVIAE SVGYASESSF HKAFVREFGC TPGEYRERVR QLAP
