RCN2_RAT
ID RCN2_RAT Reviewed; 320 AA.
AC Q62703; Q6P6X5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Reticulocalbin-2;
DE AltName: Full=Calcium-binding protein ERC-55;
DE AltName: Full=Taipoxin-associated calcium-binding protein 49;
DE Short=TCBP-49;
DE Flags: Precursor;
GN Name=Rcn2; Synonyms=Erc55;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-38 AND 130-154, AND
RP SUBUNIT.
RC STRAIN=Sprague-Dawley;
RX PubMed=7722520; DOI=10.1046/j.1471-4159.1995.64052339.x;
RA Dodds D., Schlimgen A.K., Lu S.Y., Perin M.S.;
RT "Novel reticular calcium binding protein is purified on taipoxin columns.";
RL J. Neurochem. 64:2339-2344(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 133-151, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Sprague-Dawley; TISSUE=Spinal cord;
RA Lubec G., Afjehi-Sadat L.;
RL Submitted (NOV-2006) to UniProtKB.
CC -!- FUNCTION: Not known. Binds calcium.
CC -!- SUBUNIT: Binds the snake venom phospholipase complex taipoxin.
CC {ECO:0000269|PubMed:7722520}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- SIMILARITY: Belongs to the CREC family. {ECO:0000305}.
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DR EMBL; U15734; AAA80197.1; -; mRNA.
DR EMBL; BC061962; AAH61962.1; -; mRNA.
DR PIR; I56519; I56519.
DR RefSeq; NP_058828.2; NM_017132.2.
DR AlphaFoldDB; Q62703; -.
DR BioGRID; 247896; 1.
DR CORUM; Q62703; -.
DR IntAct; Q62703; 2.
DR MINT; Q62703; -.
DR STRING; 10116.ENSRNOP00000021817; -.
DR iPTMnet; Q62703; -.
DR PhosphoSitePlus; Q62703; -.
DR jPOST; Q62703; -.
DR PaxDb; Q62703; -.
DR PRIDE; Q62703; -.
DR GeneID; 29218; -.
DR KEGG; rno:29218; -.
DR UCSC; RGD:3548; rat.
DR CTD; 5955; -.
DR RGD; 3548; Rcn2.
DR VEuPathDB; HostDB:ENSRNOG00000015780; -.
DR eggNOG; KOG4223; Eukaryota.
DR HOGENOM; CLU_044718_0_0_1; -.
DR InParanoid; Q62703; -.
DR OMA; QHNPDHD; -.
DR OrthoDB; 909079at2759; -.
DR PhylomeDB; Q62703; -.
DR PRO; PR:Q62703; -.
DR Proteomes; UP000002494; Chromosome 8.
DR Bgee; ENSRNOG00000015780; Expressed in cerebellum and 20 other tissues.
DR Genevisible; Q62703; RN.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13202; EF-hand_5; 4.
DR SMART; SM00054; EFh; 5.
DR SUPFAM; SSF47473; SSF47473; 2.
DR PROSITE; PS00018; EF_HAND_1; 5.
DR PROSITE; PS50222; EF_HAND_2; 5.
DR PROSITE; PS00014; ER_TARGET; 1.
PE 1: Evidence at protein level;
KW Calcium; Direct protein sequencing; Endoplasmic reticulum; Metal-binding;
KW Phosphoprotein; Reference proteome; Repeat; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000269|PubMed:7722520"
FT CHAIN 26..320
FT /note="Reticulocalbin-2"
FT /id="PRO_0000004150"
FT DOMAIN 64..99
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 100..135
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 150..185
FT /note="EF-hand 3"
FT /evidence="ECO:0000305"
FT DOMAIN 189..224
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 230..265
FT /note="EF-hand 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 266..301
FT /note="EF-hand 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOTIF 317..320
FT /note="Prevents secretion from ER"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10138"
FT BINDING 77
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 79
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 81
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 88
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 113
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 115
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 117
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 119
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 124
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 167
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000305"
FT BINDING 176
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000305"
FT BINDING 202
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 204
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 206
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 213
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 243
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 245
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 247
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 249
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 254
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 279
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 281
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 283
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 285
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 290
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 140
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q14257"
FT CONFLICT 17..18
FT /note="Missing (in Ref. 1; AAA80197)"
FT /evidence="ECO:0000305"
FT CONFLICT 46
FT /note="T -> A (in Ref. 1; AAA80197)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 320 AA; 37433 MW; 92CCA62B17E2D1BB CRC64;
MRLGPRPAVL GLLLLLLLYA AVAGASKAEE LHYPQGEHRA DYDRETLLGV QEDVDEYVKL
GHEEQQRRLQ SIIKKIDSDS DGFLTENELS QWIQMSFKHY AMQEAKQQFV EYDKNSDGTV
TWDEYNVQMY DRVIDFDENT ALDDTEEESF RQLHLKDKKR FEKANQDSGP GLNLEEFIAF
EHPEEVDYMT EFVIQEALEE HDKNGDGFVS LEEFLGDYRR DPTANEDPEW ILVEKDRFVN
DYDKDSDGRL DPQELLSWVV PNNQGIAQEE ALHLIDEMDL NSDKKLSEEE ILENQDLFLT
SEATDYGRQL HDDYFYHDEL
