ID   RCNA_CITK8              Reviewed;         288 AA.
AC   A8AP76;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Nickel/cobalt efflux system RcnA;
GN   Name=rcnA; OrderedLocusNames=CKO_04230;
OS   Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Citrobacter.
OX   NCBI_TaxID=290338;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-895 / CDC 4225-83 / SGSC4696;
RG   The Citrobacter koseri Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S.,
RA   Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W.,
RA   Johnson M., Thiruvilangam P., Wilson R.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Efflux system for nickel and cobalt. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- INDUCTION: By nickel and cobalt. Transcriptionally repressed by RcnR
CC       (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family. RcnA
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABV15289.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000822; ABV15289.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_024130914.1; NC_009792.1.
DR   AlphaFoldDB; A8AP76; -.
DR   STRING; 290338.CKO_04230; -.
DR   EnsemblBacteria; ABV15289; ABV15289; CKO_04230.
DR   GeneID; 45137840; -.
DR   KEGG; cko:CKO_04230; -.
DR   HOGENOM; CLU_058605_2_0_6; -.
DR   OrthoDB; 1515392at2; -.
DR   Proteomes; UP000008148; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR   Pfam; PF03824; NicO; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Cobalt; Cobalt transport;
KW   Ion transport; Membrane; Nickel; Nickel transport; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..288
FT                   /note="Nickel/cobalt efflux system RcnA"
FT                   /id="PRO_0000333782"
FT   TOPO_DOM        1..12
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        34..51
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        73..85
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        107..188
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        210..223
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        245..266
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        267..287
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        288
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          125..144
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   288 AA;  31693 MW;  B7536E6A89D71A39 CRC64;
     MGEFSTLLQQ GNAWFFIPSA ILLGVLHGLE PGHSKTMMAA FIIAIKGTIK QAVMLGLAAT
     LSHTAVVWLI ALGGMYVSRA FTAESVEPWL QLVSAIIILS TAFWMFWRTW KGERDGLANR
     LPAHTHHHHD HEHHHHDHDH DHHHDHQHVH ISLKGLTDGS HAWQDAHERA HATDIQRRFH
     DREVTNGQIL LFGLTGGLIP CPAAITVLLI CIQLKAFTLG ATMVLCFSIG LALTLVAVGV
     GAAISVQQAA KRWSGFNTLA RKAPYFSSIL IGLVGLYMGM HGYLGIIR