ID   RCNA_ECO57              Reviewed;         283 AA.
AC   Q8X3U5; Q8X369;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Nickel/cobalt efflux system RcnA;
GN   Name=rcnA; OrderedLocusNames=Z3274, ECs2912;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Efflux system for nickel and cobalt. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- INDUCTION: By nickel and cobalt. Transcriptionally repressed by RcnR
CC       (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family. RcnA
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE005174; AAG57167.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB36335.1; -; Genomic_DNA.
DR   PIR; C85838; C85838.
DR   PIR; H90992; H90992.
DR   RefSeq; NP_310939.1; NC_002695.1.
DR   RefSeq; WP_000134629.1; NZ_SEKU01000011.1.
DR   AlphaFoldDB; Q8X3U5; -.
DR   STRING; 155864.EDL933_3178; -.
DR   EnsemblBacteria; AAG57167; AAG57167; Z3274.
DR   EnsemblBacteria; BAB36335; BAB36335; ECs_2912.
DR   GeneID; 916615; -.
DR   KEGG; ece:Z3274; -.
DR   KEGG; ecs:ECs_2912; -.
DR   PATRIC; fig|386585.9.peg.3043; -.
DR   eggNOG; COG2215; Bacteria.
DR   HOGENOM; CLU_058605_2_0_6; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR   Pfam; PF03824; NicO; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Cobalt; Cobalt transport;
KW   Ion transport; Membrane; Nickel; Nickel transport; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..283
FT                   /note="Nickel/cobalt efflux system RcnA"
FT                   /id="PRO_0000194011"
FT   TOPO_DOM        1..12
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        34..56
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        78..86
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        108..184
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        206..218
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        219..239
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        240..260
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        261..281
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        282..283
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          127..162
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         138..141
FT                   /note="Missing (in strain: Sakai)"
SQ   SEQUENCE   283 AA;  31629 MW;  53B0B5CB54A5450F CRC64;
     MTEFTTLLQQ GNAWFFIPSA ILLGALHGLE PGHSKTMMAA FIIAIKGTIK QAVMLGLAAT
     ISHTAVVWLI AFGGMVISKR FTAQSAEPWL QLISAVIIIS TAFWMFWRTW RGERNWLENM
     HEHHHHDHEH HQDHDHDHDH DHDHEHHHHH EHGDNEEYQD AHARAHANDI KRRFDGREVT
     NWQILLFGLT GGLIPCPAAI TVLLICIQLK ALTLGATLVV SFSIGLALTL VTVGVGAAIS
     VQQVAKRWSG FNTLAKRAPY FSSLLIGLVG VYMGVHGFMG IMR