ID   RCNA_ECOHS              Reviewed;         274 AA.
AC   A8A1X1;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Nickel/cobalt efflux system RcnA;
GN   Name=rcnA; OrderedLocusNames=EcHS_A2242;
OS   Escherichia coli O9:H4 (strain HS).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331112;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HS;
RX   PubMed=18676672; DOI=10.1128/jb.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic analysis
RT   of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
CC   -!- FUNCTION: Efflux system for nickel and cobalt. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- INDUCTION: By nickel and cobalt. Transcriptionally repressed by RcnR
CC       (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family. RcnA
CC       subfamily. {ECO:0000305}.
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DR   EMBL; CP000802; ABV06525.1; -; Genomic_DNA.
DR   RefSeq; WP_000134650.1; NC_009800.1.
DR   AlphaFoldDB; A8A1X1; -.
DR   KEGG; ecx:EcHS_A2242; -.
DR   HOGENOM; CLU_058605_2_0_6; -.
DR   OMA; HALEPGH; -.
DR   Proteomes; UP000001123; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR   Pfam; PF03824; NicO; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Cobalt; Cobalt transport;
KW   Ion transport; Membrane; Nickel; Nickel transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..274
FT                   /note="Nickel/cobalt efflux system RcnA"
FT                   /id="PRO_0000333786"
FT   TOPO_DOM        1..12
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        34..56
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        78..86
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        108..174
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        196..209
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        210..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        231..251
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        252..272
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        273..274
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          127..153
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   274 AA;  30447 MW;  82C992DA6E2549D2 CRC64;
     MTEFTTLLQQ GNAWFFIPSV ILLGALHGLE PGHSKTMMAA FIIAIKGTIK QAVMLGLAAT
     ISHTAVVWLI AFGGMVISKR FTAQSAEPWL QLISAVIIIS TAFWMFWRTW RGERNWLENM
     HGHDYEHHHH DHEHHHDHGH HHHHEHGEYQ DAHARAHAND IKRRFDGREV TNWQILLFGL
     TGGLIPCPAA ITVLLICIQL KALTLGATLV VSFSIGLALT LVTVGVGAAI SVQQVAKRWS
     GFNTLAKRAP YFSSLLIGLV GVYMGVHGFM GIMR