ID   RCNA_ECOK1              Reviewed;         274 AA.
AC   A1ACX1;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Nickel/cobalt efflux system RcnA;
GN   Name=rcnA; OrderedLocusNames=Ecok1_20170; ORFNames=APECO1_4440;
OS   Escherichia coli O1:K1 / APEC.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=405955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17293413; DOI=10.1128/jb.01726-06;
RA   Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J.,
RA   Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.;
RT   "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7
RT   shares strong similarities with human extraintestinal pathogenic E. coli
RT   genomes.";
RL   J. Bacteriol. 189:3228-3236(2007).
CC   -!- FUNCTION: Efflux system for nickel and cobalt. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- INDUCTION: By nickel and cobalt. Transcriptionally repressed by RcnR
CC       (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family. RcnA
CC       subfamily. {ECO:0000305}.
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DR   EMBL; CP000468; ABJ01511.1; -; Genomic_DNA.
DR   RefSeq; WP_000134614.1; NC_008563.1.
DR   AlphaFoldDB; A1ACX1; -.
DR   EnsemblBacteria; ABJ01511; ABJ01511; APECO1_4440.
DR   KEGG; ecv:APECO1_4440; -.
DR   HOGENOM; CLU_058605_2_0_6; -.
DR   OMA; HALEPGH; -.
DR   Proteomes; UP000008216; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR   Pfam; PF03824; NicO; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Cobalt; Cobalt transport;
KW   Ion transport; Membrane; Nickel; Nickel transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..274
FT                   /note="Nickel/cobalt efflux system RcnA"
FT                   /id="PRO_0000333784"
FT   TOPO_DOM        1..12
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        34..56
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        78..86
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        108..174
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        196..209
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        210..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        231..251
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        252..272
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        273..274
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          127..153
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   274 AA;  30443 MW;  E7DE83A21EA2792D CRC64;
     MTEFTTLLQQ GNAWFFIPSA ILLGALHGLE PGHSKTMMAA FIIAIKGTIK QAVMLGLAAT
     ISHTAVVWLI AFGGMVISKR FTAQSAEPWL QLISAVIIIS TAFWMFWRTW RGERNWLENM
     HEHDHEHHHH DHEDHHDHGH HHHHEHGEYQ DAHARAHAND IKRRFDGREV TNWQILLFGL
     TGGLIPCPAA ITVLLICIQL KALTLGATLV VSFSLGLALT LVTVGVGAAI SVQQVAKRWS
     GFNTLAKRAP YFSSLLIGLV GVYMGVHGFM GIMR