RCNA_ECOLI
ID RCNA_ECOLI Reviewed; 274 AA.
AC P76425; O08015;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Nickel/cobalt efflux system RcnA;
GN Name=rcnA; Synonyms=yohM; OrderedLocusNames=b2106, JW2093;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=15805538; DOI=10.1128/jb.187.8.2912-2916.2005;
RA Rodrigue A., Effantin G., Mandrand-Berthelot M.A.;
RT "Identification of rcnA (yohM), a nickel and cobalt resistance gene in
RT Escherichia coli.";
RL J. Bacteriol. 187:2912-2916(2005).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP REGULATION BY RCNR.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=16956381; DOI=10.1111/j.1365-2958.2006.05369.x;
RA Iwig J.S., Rowe J.L., Chivers P.T.;
RT "Nickel homeostasis in Escherichia coli - the rcnR-rcnA efflux pathway and
RT its linkage to NikR function.";
RL Mol. Microbiol. 62:252-262(2006).
RN [7]
RP REGULATION BY RCNR AND FUR.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=17120142; DOI=10.1007/s10534-006-9039-6;
RA Koch D., Nies D.H., Grass G.;
RT "The RcnRA (YohLM) system of Escherichia coli: a connection between nickel,
RT cobalt and iron homeostasis.";
RL BioMetals 20:759-771(2007).
CC -!- FUNCTION: Efflux system for nickel and cobalt.
CC {ECO:0000269|PubMed:15805538}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15805538}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15805538}.
CC -!- INDUCTION: By nickel and cobalt. Transcriptionally repressed by RcnR.
CC Probably also regulated by Fur. Cadmium, copper and zinc have no effect
CC on the transcription. {ECO:0000269|PubMed:15805538}.
CC -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family. RcnA
CC subfamily. {ECO:0000305}.
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DR EMBL; U00096; AAC75167.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15973.1; -; Genomic_DNA.
DR PIR; A64978; A64978.
DR RefSeq; NP_416609.1; NC_000913.3.
DR RefSeq; WP_000134636.1; NZ_SSZK01000011.1.
DR AlphaFoldDB; P76425; -.
DR BioGRID; 4260955; 10.
DR STRING; 511145.b2106; -.
DR TCDB; 2.A.113.1.1; the nickel/cobalt transporter (nico) family.
DR PaxDb; P76425; -.
DR PRIDE; P76425; -.
DR EnsemblBacteria; AAC75167; AAC75167; b2106.
DR EnsemblBacteria; BAA15973; BAA15973; BAA15973.
DR GeneID; 949078; -.
DR KEGG; ecj:JW2093; -.
DR KEGG; eco:b2106; -.
DR PATRIC; fig|1411691.4.peg.141; -.
DR EchoBASE; EB3824; -.
DR eggNOG; COG2215; Bacteria.
DR HOGENOM; CLU_058605_2_0_6; -.
DR InParanoid; P76425; -.
DR OMA; HALEPGH; -.
DR PhylomeDB; P76425; -.
DR BioCyc; EcoCyc:G7138-MON; -.
DR BioCyc; MetaCyc:G7138-MON; -.
DR PRO; PR:P76425; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0046583; F:cation efflux transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0015099; F:nickel cation transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR GO; GO:0035784; P:nickel cation homeostasis; IBA:GO_Central.
DR GO; GO:0035444; P:nickel cation transmembrane transport; IMP:EcoCyc.
DR GO; GO:0032025; P:response to cobalt ion; IEP:EcoCyc.
DR GO; GO:0010045; P:response to nickel cation; IEP:EcoCyc.
DR InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR Pfam; PF03824; NicO; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Cobalt; Cobalt transport;
KW Ion transport; Membrane; Nickel; Nickel transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..274
FT /note="Nickel/cobalt efflux system RcnA"
FT /id="PRO_0000194009"
FT TOPO_DOM 1..12
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 34..56
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..86
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..209
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 231..251
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..274
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT REGION 127..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 274 AA; 30419 MW; 82C99F416E254C59 CRC64;
MTEFTTLLQQ GNAWFFIPSA ILLGALHGLE PGHSKTMMAA FIIAIKGTIK QAVMLGLAAT
ISHTAVVWLI AFGGMVISKR FTAQSAEPWL QLISAVIIIS TAFWMFWRTW RGERNWLENM
HGHDYEHHHH DHEHHHDHGH HHHHEHGEYQ DAHARAHAND IKRRFDGREV TNWQILLFGL
TGGLIPCPAA ITVLLICIQL KALTLGATLV VSFSIGLALT LVTVGVGAAI SVQQVAKRWS
GFNTLAKRAP YFSSLLIGLV GVYMGVHGFM GIMR
