RCQ1_SCHPO
ID RCQ1_SCHPO Reviewed; 656 AA.
AC O13796; Q9P7G1;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Ribosome quality control complex subunit 1 {ECO:0000250|UniProtKB:Q05468};
GN Name=rqc1 {ECO:0000250|UniProtKB:Q05468};
GN ORFNames=SPAC1142.01 {ECO:0000312|PomBase:SPAC1142.01}, SPAC17G6.18;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; SER-61; SER-63; SER-110
RP AND SER-111, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Component of the ribosome quality control complex (RQC), a
CC ribosome-associated complex that mediates ubiquitination and extraction
CC of incompletely synthesized nascent chains for proteasomal degradation.
CC Rqc1 is essential for the recruitment of cdc48 to ribosomal subunits.
CC {ECO:0000250|UniProtKB:Q05468}.
CC -!- SUBUNIT: Component of the ribosome quality control complex (RQC),
CC composed of the E3 ubiquitin ligase rkr1/ltn1, rqc1 and mtr1/rqc2, as
CC well as cdc48 and its ubiquitin-binding cofactors. RQC forms a stable
CC complex with 60S ribosomal subunits. {ECO:0000250|UniProtKB:Q05468}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the TCF25 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB16229.2; -; Genomic_DNA.
DR PIR; T37850; T37850.
DR RefSeq; NP_594265.2; NM_001019688.3.
DR AlphaFoldDB; O13796; -.
DR BioGRID; 278672; 16.
DR STRING; 4896.SPAC1142.01.1; -.
DR iPTMnet; O13796; -.
DR MaxQB; O13796; -.
DR PaxDb; O13796; -.
DR PRIDE; O13796; -.
DR EnsemblFungi; SPAC1142.01.1; SPAC1142.01.1:pep; SPAC1142.01.
DR PomBase; SPAC1142.01; rqc1.
DR VEuPathDB; FungiDB:SPAC1142.01; -.
DR eggNOG; KOG2422; Eukaryota.
DR HOGENOM; CLU_008321_1_1_1; -.
DR InParanoid; O13796; -.
DR OMA; WPPLTKN; -.
DR PhylomeDB; O13796; -.
DR PRO; PR:O13796; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:1990112; C:RQC complex; ISO:PomBase.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IGI:PomBase.
DR GO; GO:0072344; P:rescue of stalled ribosome; IBA:GO_Central.
DR GO; GO:1990116; P:ribosome-associated ubiquitin-dependent protein catabolic process; ISO:PomBase.
DR InterPro; IPR006994; TCF25/Rqc1.
DR PANTHER; PTHR22684; PTHR22684; 1.
DR Pfam; PF04910; Tcf25; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..656
FT /note="Ribosome quality control complex subunit 1"
FT /id="PRO_0000116835"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 51..122
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 634..656
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..67
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..99
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..122
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 56
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 63
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 110
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 111
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 656 AA; 75015 MW; 9C0FF49C0B6351BA CRC64;
MSSRALRKLQ RQRQTELLEE ALDSESDEDD EFSSTSGKKV VNVFEILEKE NNAINSEAEK
SVSEEEQDEP LVEGESPIVS TNKKAKNKKK KKKQQKKKKV TGKRDLDNQS SDNEKLEGLE
SSKNIDDDID EIEKAAAELK LKYREQDQVE HVAGVEESAT IPLDKELDEK LNKLLGVNIS
MLNPDLEIRK IFGRIVEKRS VNARHDNLRR KRHVLVQPQE GWPPLVRSGL GMKLTGQSQD
LECFFEITQS RAYQEVQETF EYYVQTYDPN NLLMLLRSHP FHIDTLLQVS EIIDQQGDHE
LSAELVARGL YAFDSILHPR FNLATGATRL PFAIPSNRRL FLCIWRYLQS LQSRGCWRTV
FEFCKALLQF DMSDPYAIGT CIDIYALRRR EFAWIIDFAN YLENSNKISD TPNMLYSSAL
AMFYVHGDTT DTRASMLAAF ERAPYMLSEL LDTLNISFTK SSIPSPQDPV QELHSAMYAL
YAKDSWSDPT VLAFINSILE KETVTLHDVE GQFAELTENL SRRVILLNEQ SLRKFLPQRI
LQGTILSFDP LPPDTYLSES QVFGRDISRR IASFLSDYLS RAREVNENEE EPPAHEFDLP
PAEQLLQQIE SEVGEESEDG TPVMTRLRSF FGSLFTSTNS ETEPAEESTE EMGQGD
