RCSF1_PSEAE
ID RCSF1_PSEAE Reviewed; 231 AA.
AC Q9RPT0;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Putative S-adenosylmethionine-dependent methyltransferase RcsF;
DE EC=2.1.1.-;
GN Name=rcsF; OrderedLocusNames=PA3388;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RA Campos-Garcia J., Soberon-Chavez G.;
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- SIMILARITY: Belongs to the tRNA methyltransferase O family.
CC {ECO:0000305}.
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DR EMBL; AF148964; AAD53515.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06776.1; -; Genomic_DNA.
DR PIR; G83221; G83221.
DR RefSeq; NP_252078.1; NC_002516.2.
DR RefSeq; WP_003113115.1; NZ_QZGE01000017.1.
DR PDB; 7BTU; X-ray; 1.91 A; A/B=1-231.
DR PDB; 7BTZ; X-ray; 2.40 A; A/B=1-231.
DR PDB; 7BU1; X-ray; 2.49 A; A/B=1-231.
DR PDBsum; 7BTU; -.
DR PDBsum; 7BTZ; -.
DR PDBsum; 7BU1; -.
DR AlphaFoldDB; Q9RPT0; -.
DR SMR; Q9RPT0; -.
DR STRING; 287.DR97_4534; -.
DR PaxDb; Q9RPT0; -.
DR DNASU; 880034; -.
DR EnsemblBacteria; AAG06776; AAG06776; PA3388.
DR GeneID; 880034; -.
DR KEGG; pae:PA3388; -.
DR PATRIC; fig|208964.12.peg.3547; -.
DR PseudoCAP; PA3388; -.
DR HOGENOM; CLU_013458_3_0_6; -.
DR InParanoid; Q9RPT0; -.
DR OMA; CFKEKFA; -.
DR PhylomeDB; Q9RPT0; -.
DR BioCyc; PAER208964:G1FZ6-3454-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0089715; F:tRNA m6t6A37 methyltransferase activity; IBA:GO_Central.
DR CDD; cd09281; UPF0066; 1.
DR Gene3D; 2.40.30.70; -; 1.
DR InterPro; IPR023370; TrmO-like_N.
DR InterPro; IPR041369; TrmO_C.
DR InterPro; IPR023368; UPF0066_cons_site.
DR InterPro; IPR040372; YaeB-like.
DR InterPro; IPR036413; YaeB-like_sf.
DR InterPro; IPR036414; YaeB_N_sf.
DR PANTHER; PTHR12818; PTHR12818; 1.
DR Pfam; PF01980; TrmO; 1.
DR Pfam; PF18389; TrmO_C; 1.
DR SUPFAM; SSF118196; SSF118196; 1.
DR TIGRFAMs; TIGR00104; tRNA_TsaA; 1.
DR PROSITE; PS01318; TSAA_1; 1.
DR PROSITE; PS51668; TSAA_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Methyltransferase; Reference proteome;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..231
FT /note="Putative S-adenosylmethionine-dependent
FT methyltransferase RcsF"
FT /id="PRO_0000155614"
FT DOMAIN 5..142
FT /note="TsaA-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01003"
FT BINDING 22..24
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 63..64
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 91
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 122..125
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT STRAND 3..5
FT /evidence="ECO:0007829|PDB:7BTU"
FT STRAND 8..15
FT /evidence="ECO:0007829|PDB:7BTU"
FT HELIX 25..27
FT /evidence="ECO:0007829|PDB:7BTU"
FT STRAND 32..37
FT /evidence="ECO:0007829|PDB:7BTU"
FT TURN 39..42
FT /evidence="ECO:0007829|PDB:7BTU"
FT HELIX 44..47
FT /evidence="ECO:0007829|PDB:7BTU"
FT HELIX 50..52
FT /evidence="ECO:0007829|PDB:7BTU"
FT STRAND 53..61
FT /evidence="ECO:0007829|PDB:7BTU"
FT HELIX 87..89
FT /evidence="ECO:0007829|PDB:7BTU"
FT HELIX 96..98
FT /evidence="ECO:0007829|PDB:7BTZ"
FT STRAND 99..110
FT /evidence="ECO:0007829|PDB:7BTU"
FT STRAND 113..118
FT /evidence="ECO:0007829|PDB:7BTU"
FT STRAND 126..132
FT /evidence="ECO:0007829|PDB:7BTU"
FT HELIX 135..138
FT /evidence="ECO:0007829|PDB:7BTU"
FT HELIX 147..149
FT /evidence="ECO:0007829|PDB:7BTU"
FT STRAND 156..159
FT /evidence="ECO:0007829|PDB:7BTU"
FT HELIX 161..174
FT /evidence="ECO:0007829|PDB:7BTU"
FT HELIX 178..186
FT /evidence="ECO:0007829|PDB:7BTU"
FT STRAND 203..207
FT /evidence="ECO:0007829|PDB:7BTU"
FT STRAND 210..218
FT /evidence="ECO:0007829|PDB:7BTU"
FT STRAND 221..229
FT /evidence="ECO:0007829|PDB:7BTU"
SQ SEQUENCE 231 AA; 25856 MW; C970ED16BF116B31 CRC64;
MTHSVSPIGY IRSCFMEKFA IPRQPLLAPA ARGTLELLPP FDQVEALEGL EQVSHVWLLF
LFHQALEDKP RLKVRPPRLG GNRSLGVFAT RATHRPNGIG QSVVRLEGFE AGRLWLSGID
LLDGTPVLDI KPYVPYADAV ADARNGIADA PPPGIAVEWS EQARRQAHEH GQRLRQPVAE
LIEQCLAQDP RPAYQKPEPG RRYGVRLWDL DVHWHYPRPD LIRVLDVAGG D
