RD10B_XENLA
ID RD10B_XENLA Reviewed; 341 AA.
AC Q6NRV4;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Retinol dehydrogenase 10-B;
DE EC=1.1.1.300;
GN Name=rdh10-b;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Retinol dehydrogenase with a clear preference for NADP.
CC Converts all-trans-retinol to all-trans-retinal. Has no detectable
CC activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-retinol + NADP(+) = all-trans-retinal + H(+) +
CC NADPH; Xref=Rhea:RHEA:25033, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336,
CC ChEBI:CHEBI:17898, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.1.1.300;
CC -!- PATHWAY: Cofactor metabolism; retinol metabolism.
CC -!- SUBCELLULAR LOCATION: Microsome membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}. Endoplasmic reticulum membrane
CC {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; BC070608; AAH70608.1; -; mRNA.
DR RefSeq; NP_001084938.1; NM_001091469.1.
DR AlphaFoldDB; Q6NRV4; -.
DR SMR; Q6NRV4; -.
DR DNASU; 431995; -.
DR GeneID; 431995; -.
DR KEGG; xla:431995; -.
DR CTD; 431995; -.
DR Xenbase; XB-GENE-6251537; rdh10.S.
DR OrthoDB; 1390068at2759; -.
DR UniPathway; UPA00912; -.
DR Proteomes; UP000186698; Chromosome 6S.
DR Bgee; 431995; Expressed in internal ear and 19 other tissues.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0052650; F:NADP-retinol dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0002138; P:retinoic acid biosynthetic process; IEA:InterPro.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032970; RDH10.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR PANTHER; PTHR24322:SF731; PTHR24322:SF731; 1.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Membrane; Microsome; NADP; Oxidoreductase;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..341
FT /note="Retinol dehydrogenase 10-B"
FT /id="PRO_0000307688"
FT TRANSMEM 3..23
FT /note="Helical; Signal-anchor"
FT /evidence="ECO:0000255"
FT ACT_SITE 210
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 40..64
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 197
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 341 AA; 38530 MW; F56598AA51239216 CRC64;
MHIVLEFFLV TFRVLWAFVL AAAKWFVRPK DKNVAGQVCL ITGAGSGLGR LFALEFARRR
AQLVLWDINP QSNEETADMV RDIYRQLQAE DSARRANSSA DEEVLPCCNL QVYTYTCDVG
KRESVYSTAE RVRREVGDVY LLLNNAGVVS GHHLLECPDE LIERTMMVNC HAHFWTTKAF
LPKMMEMNHG HIVSVASSLG LFSTAGVEDY CASKFGVVGF HESLSHELKA ADKDGIKTTL
VCPYLVDTGM FRGCRIRKEI EPFLPPLKPD YCVKQAMRAI LTDQPMICTP RLMYIVLCMK
SILPFEAVVC MYRFLGADKC MYPFIAQRKQ ATNNNETKNG I