ID   RD15_PHYIT              Reviewed;         242 AA.
AC   D0NVI0;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=RxLR effector protein PexRD15 {ECO:0000303|PubMed:19794118};
DE   Flags: Precursor;
GN   Name=PexRD15 {ECO:0000303|PubMed:19794118}; ORFNames=PITG_16737;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   INDUCTION, AND DOMAIN.
RX   PubMed=17914356; DOI=10.1038/nature06203;
RA   Whisson S.C., Boevink P.C., Moleleki L., Avrova A.O., Morales J.G.,
RA   Gilroy E.M., Armstrong M.R., Grouffaud S., van West P., Chapman S.,
RA   Hein I., Toth I.K., Pritchard L., Birch P.R.;
RT   "A translocation signal for delivery of oomycete effector proteins into
RT   host plant cells.";
RL   Nature 450:115-118(2007).
RN   [3]
RP   INDUCTION.
RX   PubMed=19794118; DOI=10.1105/tpc.109.068247;
RA   Oh S.K., Young C., Lee M., Oliva R., Bozkurt T.O., Cano L.M., Win J.,
RA   Bos J.I., Liu H.Y., van Damme M., Morgan W., Choi D., Van der Vossen E.A.,
RA   Vleeshouwers V.G., Kamoun S.;
RT   "In planta expression screens of Phytophthora infestans RXLR effectors
RT   reveal diverse phenotypes, including activation of the Solanum
RT   bulbocastanum disease resistance protein Rpi-blb2.";
RL   Plant Cell 21:2928-2947(2009).
RN   [4]
RP   INDUCTION.
RX   PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA   Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT   "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT   dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT   and a major role for calcium signaling in development.";
RL   BMC Genomics 18:198-198(2017).
RN   [5]
RP   INDUCTION.
RX   PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA   Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT   "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT   early stage of potato infection are suppressive to host defense.";
RL   Front. Plant Sci. 8:2155-2155(2017).
RN   [6]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30329083; DOI=10.1093/jxb/ery360;
RA   Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA   van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA   Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA   Birch P.R.J., Boevink P.C.;
RT   "Phytophthora infestans RXLR effectors act in concert at diverse
RT   subcellular locations to enhance host colonization.";
RL   J. Exp. Bot. 70:343-356(2019).
CC   -!- FUNCTION: Effector that enhances P.infestans colonization of Nicotiana
CC       benthamiana leaves. {ECO:0000269|PubMed:30329083}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host cell
CC       membrane {ECO:0000269|PubMed:30329083}.
CC   -!- INDUCTION: Expression is induced during host plant infection.
CC       {ECO:0000269|PubMed:17914356, ECO:0000269|PubMed:19794118,
CC       ECO:0000269|PubMed:28228125, ECO:0000269|PubMed:29312401}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:17914356}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; DS028168; EEY66657.1; -; Genomic_DNA.
DR   RefSeq; XP_002896958.1; XM_002896912.1.
DR   AlphaFoldDB; D0NVI0; -.
DR   SMR; D0NVI0; -.
DR   EnsemblProtists; PITG_16737T0; PITG_16737T0; PITG_16737.
DR   GeneID; 9465522; -.
DR   KEGG; pif:PITG_16737; -.
DR   VEuPathDB; FungiDB:PITG_16737; -.
DR   eggNOG; ENOG502STM1; Eukaryota.
DR   HOGENOM; CLU_1196877_0_0_1; -.
DR   InParanoid; D0NVI0; -.
DR   OMA; TRELNMR; -.
DR   OrthoDB; 1547628at2759; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome; Secreted;
KW   Signal; Virulence.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..242
FT                   /note="RxLR effector protein PexRD15"
FT                   /id="PRO_5003013691"
FT   MOTIF           48..66
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:17914356"
SQ   SEQUENCE   242 AA;  27593 MW;  E0F6384EC4E77CF9 CRC64;
     MMKSLYAVNL VLLLLLAFFA PAPATRELNM RAAPSDSTRV VDYATTERLL RAHSSDKEEQ
     KEEEERAISI NFSSLEKIFK KVTSAKTTEL QGMLKADEAL GSAFKTLKLG TMRIGKDGSV
     DPKMVAKFLS SRNFKIWSQH AVKINKDDPY GEMLKALTNV FGEKNVAMMI LVGNLSRNSR
     DVAKKLEKAQ FYKWYFVDKY KTADEVFTNV LKADRNRIHG YGREKEIWGD YAKYVTTTVM
     KY