ID   RD18_PHYIT              Reviewed;         484 AA.
AC   D0N4Y1;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=RxLR effector protein PexRD18 {ECO:0000303|PubMed:19794118};
DE   Flags: Precursor;
GN   Name=PexRD18 {ECO:0000303|PubMed:19794118}; ORFNames=PITG_06478;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   INDUCTION, AND DOMAIN.
RX   PubMed=17914356; DOI=10.1038/nature06203;
RA   Whisson S.C., Boevink P.C., Moleleki L., Avrova A.O., Morales J.G.,
RA   Gilroy E.M., Armstrong M.R., Grouffaud S., van West P., Chapman S.,
RA   Hein I., Toth I.K., Pritchard L., Birch P.R.;
RT   "A translocation signal for delivery of oomycete effector proteins into
RT   host plant cells.";
RL   Nature 450:115-118(2007).
RN   [3]
RP   INDUCTION.
RX   PubMed=19794118; DOI=10.1105/tpc.109.068247;
RA   Oh S.K., Young C., Lee M., Oliva R., Bozkurt T.O., Cano L.M., Win J.,
RA   Bos J.I., Liu H.Y., van Damme M., Morgan W., Choi D., Van der Vossen E.A.,
RA   Vleeshouwers V.G., Kamoun S.;
RT   "In planta expression screens of Phytophthora infestans RXLR effectors
RT   reveal diverse phenotypes, including activation of the Solanum
RT   bulbocastanum disease resistance protein Rpi-blb2.";
RL   Plant Cell 21:2928-2947(2009).
RN   [4]
RP   INDUCTION.
RX   PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA   Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT   "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT   dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT   and a major role for calcium signaling in development.";
RL   BMC Genomics 18:198-198(2017).
RN   [5]
RP   INDUCTION.
RX   PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA   Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT   "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT   early stage of potato infection are suppressive to host defense.";
RL   Front. Plant Sci. 8:2155-2155(2017).
RN   [6]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=30329083; DOI=10.1093/jxb/ery360;
RA   Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA   van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA   Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA   Birch P.R.J., Boevink P.C.;
RT   "Phytophthora infestans RXLR effectors act in concert at diverse
RT   subcellular locations to enhance host colonization.";
RL   J. Exp. Bot. 70:343-356(2019).
CC   -!- FUNCTION: Effector that enhances P.infestans colonization of Nicotiana
CC       benthamiana leaves. {ECO:0000269|PubMed:30329083}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host cell
CC       membrane {ECO:0000269|PubMed:30329083}.
CC   -!- INDUCTION: Expression is induced during host plant infection.
CC       {ECO:0000269|PubMed:17914356, ECO:0000269|PubMed:19741609,
CC       ECO:0000269|PubMed:19794118, ECO:0000269|PubMed:28228125,
CC       ECO:0000269|PubMed:29312401}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:17914356}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; DS028125; EEY69939.1; -; Genomic_DNA.
DR   RefSeq; XP_002998586.1; XM_002998540.1.
DR   AlphaFoldDB; D0N4Y1; -.
DR   SMR; D0N4Y1; -.
DR   EnsemblProtists; PITG_06478T0; PITG_06478T0; PITG_06478.
DR   GeneID; 9471688; -.
DR   KEGG; pif:PITG_06478; -.
DR   VEuPathDB; FungiDB:PITG_06478; -.
DR   eggNOG; ENOG502T14E; Eukaryota.
DR   HOGENOM; CLU_044421_1_0_1; -.
DR   InParanoid; D0N4Y1; -.
DR   OMA; NEERWIG; -.
DR   OrthoDB; 1258663at2759; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR040786; RXLR_WY.
DR   Pfam; PF18634; RXLR_WY; 3.
PE   2: Evidence at transcript level;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome; Secreted;
KW   Signal; Virulence.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..484
FT                   /note="RxLR effector protein PexRD18"
FT                   /id="PRO_5003012955"
FT   MOTIF           55..79
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:17914356"
SQ   SEQUENCE   484 AA;  55782 MW;  352C8AD8AC96D145 CRC64;
     MSHQRILLLL MAAFFAWVSA QTPPGQADKS KLIAHDVLMK TTSLSETTIA TSSKRFLRLY
     DAEVRDTVRG DNDVDREERG TTPLLSKVDD LIHKVFKSNP EQAQIKAWMK SRVHPQAIFD
     TLRLAKSTTK LNDDPNLLLW LKLVAAFRAK NGNQAFSDLD LYYLLLKRSS GEELKILIES
     FRKTGALKEL GKSMQKSLSG SWVSKTLQHE TDPKIVYDTL RLQEAGTKLV DSPIFHQWLA
     YAQQYRAQKG NHWFGDDDML DLFRKTMPEK DVVTLLHLLR NVPGMKDHGD TMQRFLFLSS
     KTSRKMMHDV WLNYDVTPEQ VFKILRLVKV NMDAVDTNAM FIHWLRYVNL YRSHTKKNVL
     SSVQMVHFLA DTKPLRSEWQ FATFFESLKD VPDLKRLAEN MQTYLFQNWL HTEWDPKAVS
     SMLAIPFPTS AVYLPKNDPI YKTWVAYTLY YTERKGGVSL LNKVKTLLDN DNPIGALTAA
     MKAQ