ID   RD41_PHYIT              Reviewed;         102 AA.
AC   D0N0I9;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2009, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=RxLR effector protein PexRD41 {ECO:0000303|PubMed:19794118};
DE   Flags: Precursor;
GN   Name=PexRD41 {ECO:0000303|PubMed:19794118}; ORFNames=PITG_04089;
OS   Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=403677;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=T30-4;
RX   PubMed=19741609; DOI=10.1038/nature08358;
RG   The Broad Institute Genome Sequencing Platform;
RA   Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA   Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA   Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA   Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA   Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA   Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA   Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA   Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA   Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA   Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA   Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA   Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA   Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA   Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA   Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA   Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA   Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA   Nusbaum C.;
RT   "Genome sequence and analysis of the Irish potato famine pathogen
RT   Phytophthora infestans.";
RL   Nature 461:393-398(2009).
RN   [2]
RP   INDUCTION, AND DOMAIN.
RX   PubMed=17914356; DOI=10.1038/nature06203;
RA   Whisson S.C., Boevink P.C., Moleleki L., Avrova A.O., Morales J.G.,
RA   Gilroy E.M., Armstrong M.R., Grouffaud S., van West P., Chapman S.,
RA   Hein I., Toth I.K., Pritchard L., Birch P.R.;
RT   "A translocation signal for delivery of oomycete effector proteins into
RT   host plant cells.";
RL   Nature 450:115-118(2007).
RN   [3]
RP   INDUCTION.
RX   PubMed=19794118; DOI=10.1105/tpc.109.068247;
RA   Oh S.K., Young C., Lee M., Oliva R., Bozkurt T.O., Cano L.M., Win J.,
RA   Bos J.I., Liu H.Y., van Damme M., Morgan W., Choi D., Van der Vossen E.A.,
RA   Vleeshouwers V.G., Kamoun S.;
RT   "In planta expression screens of Phytophthora infestans RXLR effectors
RT   reveal diverse phenotypes, including activation of the Solanum
RT   bulbocastanum disease resistance protein Rpi-blb2.";
RL   Plant Cell 21:2928-2947(2009).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH POTATO KRBP1.
RX   PubMed=25936676; DOI=10.1016/j.molp.2015.04.012;
RA   Wang X., Boevink P., McLellan H., Armstrong M., Bukharova T., Qin Z.,
RA   Birch P.R.;
RT   "A host KH RNA-binding protein is a susceptibility factor targeted by an
RT   RXLR effector to promote late blight disease.";
RL   Mol. Plant 8:1385-1395(2015).
RN   [5]
RP   INDUCTION.
RX   PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA   Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT   "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT   dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT   and a major role for calcium signaling in development.";
RL   BMC Genomics 18:198-198(2017).
RN   [6]
RP   INDUCTION.
RX   PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA   Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT   "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT   early stage of potato infection are suppressive to host defense.";
RL   Front. Plant Sci. 8:2155-2155(2017).
CC   -!- FUNCTION: Effector that enhances P.infestans colonization of host plant
CC       leaves (PubMed:25936676). During the early stages of P.infestans
CC       infection, interacts with and stabilizes host potato K-homology (KH)
CC       RNA-binding protein KRBP1, leading to its accumulation
CC       (PubMed:25936676). {ECO:0000269|PubMed:25936676}.
CC   -!- SUBUNIT: Interacts with host KRBP1. {ECO:0000269|PubMed:25936676}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:25936676}. Host
CC       cytoplasm {ECO:0000269|PubMed:25936676}. Host nucleus, host nucleolus
CC       {ECO:0000269|PubMed:25936676}. Host nucleus
CC       {ECO:0000269|PubMed:25936676}. Note=Forms a ring around the nucleolus.
CC       Nuclear localization is necessary to promote P.infestans colonization.
CC       {ECO:0000269|PubMed:25936676}.
CC   -!- INDUCTION: Expression is induced during host plant infection.
CC       {ECO:0000269|PubMed:17914356, ECO:0000269|PubMed:19741609,
CC       ECO:0000269|PubMed:19794118, ECO:0000269|PubMed:28228125,
CC       ECO:0000269|PubMed:29312401}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:17914356}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; DS028122; EEY67152.1; -; Genomic_DNA.
DR   RefSeq; XP_002905800.1; XM_002905754.1.
DR   AlphaFoldDB; D0N0I9; -.
DR   EnsemblProtists; PITG_04089T0; PITG_04089T0; PITG_04089.
DR   GeneID; 9479506; -.
DR   KEGG; pif:PITG_04089; -.
DR   VEuPathDB; FungiDB:PITG_04089; -.
DR   HOGENOM; CLU_158959_0_0_1; -.
DR   Proteomes; UP000006643; Partially assembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Host nucleus; Reference proteome; Secreted; Signal;
KW   Virulence.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..102
FT                   /note="RxLR effector protein PexRD41"
FT                   /id="PRO_5003012171"
FT   MOTIF           39..53
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:17914356"
SQ   SEQUENCE   102 AA;  11199 MW;  AA785B60F2EA0BDA CRC64;
     MRSIFYFALA FAALTCSNAS AALPNPDETR LLSDTFTKRS LRVAGQEAAR GEEIVRVTAQ
     STNKIFKRPA EKDMSKLIAA AKKALLEKKM AKLSKVIKKP AK