RD522_ARATH
ID RD522_ARATH Reviewed; 199 AA.
AC Q9FIJ4; Q8LAJ6;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=DNA repair RAD52-like protein 2, chloroplastic {ECO:0000305};
DE AltName: Full=Organellar DNA-binding protein 2 {ECO:0000303|PubMed:22762281};
DE Flags: Precursor;
GN Name=RAD52-2 {ECO:0000303|PubMed:22202891};
GN Synonyms=ODB2 {ECO:0000303|PubMed:22762281};
GN OrderedLocusNames=At5g47870 {ECO:0000312|Araport:AT5G47870};
GN ORFNames=MCA23.21 {ECO:0000312|EMBL:BAB11334.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=22202891; DOI=10.1105/tpc.111.091744;
RA Samach A., Melamed-Bessudo C., Avivi-Ragolski N., Pietrokovski S.,
RA Levy A.A.;
RT "Identification of plant RAD52 homologs and characterization of the
RT Arabidopsis thaliana RAD52-like genes.";
RL Plant Cell 23:4266-4279(2011).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-41, CLEAVAGE OF TRANSIT PEPTIDE
RP [LARGE SCALE ANALYSIS] AFTER CYS-40, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=22762281; DOI=10.1111/j.1365-313x.2012.05097.x;
RA Janicka S., Kuehn K., Le Ret M., Bonnard G., Imbault P., Augustyniak H.,
RA Gualberto J.M.;
RT "A RAD52-like single-stranded DNA binding protein affects mitochondrial DNA
RT repair by recombination.";
RL Plant J. 72:423-435(2012).
CC -!- FUNCTION: Involved in double-stranded DNA break repair.
CC {ECO:0000269|PubMed:22202891}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:22202891, ECO:0000269|PubMed:22762281}.
CC Note=Distributed in a punctate pattern within chloroplast.
CC {ECO:0000269|PubMed:22202891}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and shoots. Expressed at low
CC levels in cauline leaves, flower buds, flowers and siliques.
CC {ECO:0000269|PubMed:22202891}.
CC -!- DISRUPTION PHENOTYPE: Reduced fertility, increased sensitivity to
CC mitomycin C, and decreased levels of intrachromosomal recombination.
CC {ECO:0000269|PubMed:22202891}.
CC -!- SIMILARITY: Belongs to the RAD52 family. {ECO:0000305}.
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DR EMBL; AB016886; BAB11334.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95582.1; -; Genomic_DNA.
DR EMBL; AK175664; BAD43427.1; -; mRNA.
DR EMBL; BT024916; ABD94072.1; -; mRNA.
DR EMBL; AY087773; AAM65309.1; -; mRNA.
DR RefSeq; NP_199598.1; NM_124161.3.
DR AlphaFoldDB; Q9FIJ4; -.
DR SMR; Q9FIJ4; -.
DR STRING; 3702.AT5G47870.1; -.
DR iPTMnet; Q9FIJ4; -.
DR PaxDb; Q9FIJ4; -.
DR PRIDE; Q9FIJ4; -.
DR ProteomicsDB; 225922; -.
DR EnsemblPlants; AT5G47870.1; AT5G47870.1; AT5G47870.
DR GeneID; 834838; -.
DR Gramene; AT5G47870.1; AT5G47870.1; AT5G47870.
DR KEGG; ath:AT5G47870; -.
DR Araport; AT5G47870; -.
DR TAIR; locus:2160967; AT5G47870.
DR eggNOG; ENOG502RXVC; Eukaryota.
DR HOGENOM; CLU_095139_0_1_1; -.
DR InParanoid; Q9FIJ4; -.
DR OMA; HSTFIPW; -.
DR OrthoDB; 1463263at2759; -.
DR PhylomeDB; Q9FIJ4; -.
DR PRO; PR:Q9FIJ4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FIJ4; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IMP:TAIR.
DR InterPro; IPR037489; RAD52-like.
DR PANTHER; PTHR34050; PTHR34050; 1.
PE 1: Evidence at protein level;
KW Acetylation; Chloroplast; DNA damage; DNA recombination; DNA repair;
KW DNA-binding; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..40
FT /note="Chloroplast"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 41..199
FT /note="DNA repair RAD52-like protein 2, chloroplastic"
FT /id="PRO_0000438187"
FT MOD_RES 41
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CONFLICT 5
FT /note="V -> M (in Ref. 5; AAM65309)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="D -> Y (in Ref. 5; AAM65309)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 199 AA; 21409 MW; F2E5686836FF01B7 CRC64;
MALQVQQTSA AFTISSPSTA AARIKLSPFR TVAVNRGVRC SGGGVGGGDA GKKKAVPNSN
YVVPIDKFSS SSSITRPLIE ILRDLNKKIP DNIVKSHDPP STSAATSGFI PWYHANRMLS
FYAPGWCGEV RDVIFSENGN VTVVYRLTIR GSDGEAHRES TGTVTTTDDH IEDPVTAAEE
IAFCRACARF GLGLYLYHE
