ID   RDE10_CAEEL             Reviewed;         627 AA.
AC   Q9N3S2;
DT   11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=RNA interference defective protein 10 {ECO:0000312|WormBase:Y47G6A.4};
GN   Name=rde-10 {ECO:0000312|WormBase:Y47G6A.4};
GN   ORFNames=Y47G6A.4 {ECO:0000312|WormBase:Y47G6A.4};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, INTERACTION WITH RDE-11 AND ERGO-1, AND DISRUPTION PHENOTYPE.
RX   PubMed=22542102; DOI=10.1016/j.cub.2012.04.011;
RA   Zhang C., Montgomery T.A., Fischer S.E., Garcia S.M., Riedel C.G.,
RA   Fahlgren N., Sullivan C.M., Carrington J.C., Ruvkun G.;
RT   "The Caenorhabditis elegans RDE-10/RDE-11 complex regulates RNAi by
RT   promoting secondary siRNA amplification.";
RL   Curr. Biol. 22:881-890(2012).
RN   [3] {ECO:0000305}
RP   FUNCTION, AND INTERACTION WITH RDE-11.
RX   PubMed=22508728; DOI=10.1101/gad.180679.111;
RA   Yang H., Zhang Y., Vallandingham J., Li H., Li H., Florens L., Mak H.Y.;
RT   "The RDE-10/RDE-11 complex triggers RNAi-induced mRNA degradation by
RT   association with target mRNA in C. elegans.";
RL   Genes Dev. 26:846-856(2012).
CC   -!- FUNCTION: In complex with rde-11, required in the endogenous and
CC       exogenous siRNA pathway for biogenesis and accumulation of secondary
CC       small interfering RNA (siRNA) intermediates, such as 22G-siRNAs derived
CC       from ergo-1 targets. {ECO:0000269|PubMed:22508728,
CC       ECO:0000269|PubMed:22542102}.
CC   -!- SUBUNIT: Interacts with rde-11 (via RING-type zinc finger domain)
CC       (PubMed:22508728, PubMed:22542102). Interacts with ergo-1
CC       (PubMed:22542102). {ECO:0000269|PubMed:22508728,
CC       ECO:0000269|PubMed:22542102}.
CC   -!- DISRUPTION PHENOTYPE: Insensitive to RNAi-mediated gene silencing.
CC       {ECO:0000269|PubMed:22542102}.
CC   -!- SIMILARITY: Belongs to the maelstrom family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BX284601; CCD72548.1; -; Genomic_DNA.
DR   RefSeq; NP_491178.1; NM_058777.6.
DR   AlphaFoldDB; Q9N3S2; -.
DR   ComplexPortal; CPX-1000; Rde-10/Rde-11 complex.
DR   STRING; 6239.Y47G6A.4; -.
DR   EPD; Q9N3S2; -.
DR   PaxDb; Q9N3S2; -.
DR   PeptideAtlas; Q9N3S2; -.
DR   EnsemblMetazoa; Y47G6A.4.1; Y47G6A.4.1; WBGene00021634.
DR   GeneID; 171923; -.
DR   KEGG; cel:CELE_Y47G6A.4; -.
DR   UCSC; Y47G6A.4; c. elegans.
DR   CTD; 171923; -.
DR   WormBase; Y47G6A.4; CE24371; WBGene00021634; rde-10.
DR   eggNOG; ENOG502QQDI; Eukaryota.
DR   HOGENOM; CLU_436308_0_0_1; -.
DR   InParanoid; Q9N3S2; -.
DR   OrthoDB; 1411703at2759; -.
DR   PRO; PR:Q9N3S2; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00021634; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0031332; C:RNAi effector complex; IC:ComplexPortal.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0060964; P:regulation of miRNA-mediated gene silencing; IEA:InterPro.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   InterPro; IPR024970; Maelstrom.
DR   Pfam; PF13017; Maelstrom; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Repressor; RNA-mediated gene silencing;
KW   Translation regulation.
FT   CHAIN           1..627
FT                   /note="RNA interference defective protein 10"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000434604"
FT   REGION          1..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          467..487
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          523..589
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        17..31
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        467..482
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        559..576
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   627 AA;  72155 MW;  837CA8E90358A644 CRC64;
     MSNHRSNFRD YQREGIRANN AGTSGDAVRQ NGNPISVAKH VDGKKSVYML FLRQIGQKKF
     LTEQGHRYNQ NDQADKDIMT RYYHGMCPDL KQKFEREVAE HNGNRGLVIK TKHQRAQRNR
     EMRHRNPDEF QQLRRAHLET LSQTPSVIAL PRDINHVLHI TEDLDAFCLA NRKKAKRIMT
     SYIAQRSDDP GNPLLCEDYT MQIVSVFPVA YAFKPSINKL SSYPAEISVT TFNLKNGIIQ
     NESRFVKFDA AWFYPDADDI GHEELSRKAM ADELGISPNG PADGCEPYEV FEWLQHLLKQ
     HPKSPILCDR AQFNFVYYGI KTLATYTGIN AITFFQEVII PSILSIQDFT SVILEKAPTD
     VPRVWRDVDI CNQFQYHFLI PRTELNLFCS FHENKPSPTK YNCVKAHNAR LLDNFFTVIK
     GNRLQGFVIS PPVHEICIQD GSDTSLPQTI LARTISRNDA EVYAARQRDT DEQYDVHQEG
     PSNHDQYEFA SEPLDFEEDS DEENYNEQLD VPYSYNDHFI SSSSVREPEH PSARSRDVAP
     NVQQESVVVP APRRLSPQRA PRPSQNSPNA YSERKSFSAF PSEDPSEDYE TPIISHIMNR
     NEADQYFNIL DSVKPGQKYK IIKFDDF