RDF_BPPHC
ID RDF_BPPHC Reviewed; 244 AA.
AC Q9T216;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 23-FEB-2022, entry version 67.
DE RecName: Full=Recombination directionality factor {ECO:0000305};
DE Short=RDF;
DE AltName: Full=Gene product 3 {ECO:0000305};
DE Short=gp3;
GN Name=3 {ECO:0000312|EMBL:CAA07127.2};
GN Synonyms=rdf {ECO:0000312|EMBL:AFR23342.1};
OS Streptomyces phage phiC31 (Bacteriophage phi-C31).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae; Lomovskayavirus.
OX NCBI_TaxID=10719 {ECO:0000312|Proteomes:UP000002124};
OH NCBI_TaxID=1902; Streptomyces coelicolor.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Norwich stock {ECO:0000312|EMBL:CAA07127.2};
RX PubMed=10219087; DOI=10.1093/nar/27.10.2145;
RA Smith M.C., Burns R.N., Wilson S.E., Gregory M.A.;
RT "The complete genome sequence of the Streptomyces temperate phage straight
RT phiC31: evolutionary relationships to other viruses.";
RL Nucleic Acids Res. 27:2145-2155(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Norwich stock {ECO:0000312|EMBL:CAA07127.2};
RX PubMed=10051617; DOI=10.1073/pnas.96.5.2192;
RA Hendrix R.W., Smith M.C.M., Burns N., Ford M.E., Hatfull G.F.;
RT "Evolutionary relationships among diverse bacteriophages and prophages: all
RT the world's a phage.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:2192-2197(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=22933343; DOI=10.1002/biot.201200283;
RA Farruggio A.P., Chavez C.L., Mikell C.L., Calos M.P.;
RT "Efficient reversal of phiC31 integrase recombination in mammalian cells.";
RL Biotechnol. J. 7:1332-1336(2012).
RN [4] {ECO:0000312|EMBL:CAA07127.2}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Norwich stock {ECO:0000312|EMBL:CAA07127.2};
RA Smith M.C.M.;
RL Submitted (JUN-2012) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INTERACTION WITH INTEGRASE, AND INDUCTION.
RX PubMed=21564337; DOI=10.1111/j.1365-2958.2011.07696.x;
RA Khaleel T., Younger E., McEwan A.R., Varghese A.S., Smith M.C.;
RT "A phage protein that binds phiC31 integrase to switch its
RT directionality.";
RL Mol. Microbiol. 80:1450-1463(2011).
CC -!- FUNCTION: Recombination directionality factor that interacts directly
CC with the integrase tetramer to activate excision and inhibit
CC integration. {ECO:0000269|PubMed:21564337}.
CC -!- SUBUNIT: Interacts with the integrase. {ECO:0000269|PubMed:21564337}.
CC -!- INDUCTION: Expressed early in the viral replication cycle.
CC {ECO:0000269|PubMed:21564337}.
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DR EMBL; JX120614; AFR23342.1; -; Genomic_DNA.
DR EMBL; AJ006589; CAA07127.2; -; Genomic_DNA.
DR RefSeq; NP_047948.2; NC_001978.3.
DR GeneID; 2715894; -.
DR KEGG; vg:2715894; -.
DR Proteomes; UP000002124; Genome.
DR GO; GO:0032359; P:provirus excision; IEA:UniProtKB-KW.
DR InterPro; IPR043991; Gp3-like.
DR Pfam; PF18897; Gp3-like; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Viral genome excision.
FT CHAIN 1..244
FT /note="Recombination directionality factor"
FT /id="PRO_0000433208"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..25
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 244 AA; 27328 MW; DC4440399CEE9C95 CRC64;
MAKRSIWAGD EDNKPKKRET YADDTVGRFH SGYSETNERG KVVPVALDKW RISTGEQSVA
DAVAQLFGGT PVENEESTSE NFIDVFTDRP KVPVIIEADG IHWDMKLWLN GKLKHHCDGF
DFVSHADEEM IGQPCGCPKL FDERKAAAKE YDAPNPAITV TFTLADDPEL GRFKFQTGSW
TLFKVLHEAE DDVERVGKGG AVLANLELEL VEYTPKRGPM RNKLVSYYKP TITVLKSYND
AIAD
