ID   RDH10_XENTR             Reviewed;         341 AA.
AC   Q5XGF7;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Retinol dehydrogenase 10;
DE            EC=1.1.1.300;
GN   Name=rdh10;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Retinol dehydrogenase with a clear preference for NADP.
CC       Converts all-trans-retinol to all-trans-retinal. Has no detectable
CC       activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-retinol + NADP(+) = all-trans-retinal + H(+) +
CC         NADPH; Xref=Rhea:RHEA:25033, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336,
CC         ChEBI:CHEBI:17898, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.300;
CC   -!- PATHWAY: Cofactor metabolism; retinol metabolism.
CC   -!- SUBCELLULAR LOCATION: Microsome membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}. Endoplasmic reticulum membrane
CC       {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; BC084483; AAH84483.1; -; mRNA.
DR   RefSeq; NP_001011091.1; NM_001011091.1.
DR   AlphaFoldDB; Q5XGF7; -.
DR   SMR; Q5XGF7; -.
DR   STRING; 8364.ENSXETP00000025854; -.
DR   PaxDb; Q5XGF7; -.
DR   DNASU; 496504; -.
DR   Ensembl; ENSXETT00000025854; ENSXETP00000025854; ENSXETG00000011825.
DR   GeneID; 496504; -.
DR   KEGG; xtr:496504; -.
DR   CTD; 157506; -.
DR   Xenbase; XB-GENE-944961; rdh10.
DR   eggNOG; KOG1201; Eukaryota.
DR   HOGENOM; CLU_010194_2_5_1; -.
DR   InParanoid; Q5XGF7; -.
DR   OMA; HICAPRV; -.
DR   OrthoDB; 1373099at2759; -.
DR   PhylomeDB; Q5XGF7; -.
DR   TreeFam; TF312837; -.
DR   Reactome; R-XTR-2453902; The canonical retinoid cycle in rods (twilight vision).
DR   Reactome; R-XTR-5365859; RA biosynthesis pathway.
DR   UniPathway; UPA00912; -.
DR   Proteomes; UP000008143; Chromosome 6.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000011825; Expressed in gastrula and 12 other tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005811; C:lipid droplet; IBA:GO_Central.
DR   GO; GO:0052650; F:NADP-retinol dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IBA:GO_Central.
DR   GO; GO:0002138; P:retinoic acid biosynthetic process; IEA:InterPro.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR032970; RDH10.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PANTHER; PTHR24322:SF731; PTHR24322:SF731; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Lipid metabolism; Membrane; Microsome; NADP;
KW   Oxidoreductase; Reference proteome; Signal-anchor; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..341
FT                   /note="Retinol dehydrogenase 10"
FT                   /id="PRO_0000307689"
FT   TRANSMEM        3..23
FT                   /note="Helical; Signal-anchor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        210
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         40..64
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         197
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   341 AA;  38424 MW;  5E97AF00EE1BCD09 CRC64;
     MHIVLEFFLV TFKVLWAFVL AAAKWLVRPK DKSVAGQVCL ITGAGSGLGR LFALEFARRR
     AQLVLWDINS QSNEETAEMV RSIYRELEAE DSARRAGNAT EEEVQPCCNF QVYTYTCDVG
     KRESVYSTAE RVRREVGDVY LLLNNAGVVS GHHLLECPDE LIERTMMVNC HAHFWTTKAF
     LPKMMEMNHG HIVSVASSLG LFSTAGVEDY CASKFGVVGF HESLSHELKA ADKDGIKTTL
     VCPYLVDTGM FRGCRIRKEI EPFLPPLKPD YCVKQAMRAI LTDQPMICTP RLMYIVTCMK
     SILPFEAVVC MYRFLGADKC MYPFIAQRKQ ATNNNEAKNG I