RDH12_BOVIN
ID RDH12_BOVIN Reviewed; 316 AA.
AC P59837;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 29-AUG-2003, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Retinol dehydrogenase 12;
DE EC=1.1.1.300 {ECO:0000250|UniProtKB:Q96NR8};
DE AltName: Full=Double substrate specificity short-chain dehydrogenase/reductase 2 {ECO:0000303|PubMed:12226107};
GN Name=RDH12; Synonyms=DSSDR2;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Retina;
RX PubMed=12226107; DOI=10.1074/jbc.m208882200;
RA Haeseleer F., Jang G.-F., Imanishi Y., Driessen C.A.G.G., Matsumura M.,
RA Nelson P.S., Palczewski K.;
RT "Dual-substrate specificity short chain retinol dehydrogenases from the
RT vertebrate retina.";
RL J. Biol. Chem. 277:45537-45546(2002).
CC -!- FUNCTION: Retinoids dehydrogenase/reductase with a clear preference for
CC NADP. Displays high activity towards 9-cis, 11-cis and all-trans-
CC retinal. Shows very weak activity towards 13-cis-retinol. Also exhibits
CC activity, albeit with lower affinity than for retinaldehydes, towards
CC lipid peroxidation products (C9 aldehydes) such as 4-hydroxynonenal and
CC trans-2-nonenal. May play an important function in photoreceptor cells
CC to detoxify 4-hydroxynonenal and potentially other toxic aldehyde
CC products resulting from lipid peroxidation. Has no dehydrogenase
CC activity towards steroids. {ECO:0000250|UniProtKB:Q96NR8}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-retinol + NADP(+) = all-trans-retinal + H(+) +
CC NADPH; Xref=Rhea:RHEA:25033, ChEBI:CHEBI:15378, ChEBI:CHEBI:17336,
CC ChEBI:CHEBI:17898, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.1.1.300; Evidence={ECO:0000250|UniProtKB:Q96NR8};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=11-cis-retinol + NADP(+) = 11-cis-retinal + H(+) + NADPH;
CC Xref=Rhea:RHEA:54912, ChEBI:CHEBI:15378, ChEBI:CHEBI:16066,
CC ChEBI:CHEBI:16302, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC Evidence={ECO:0000250|UniProtKB:Q96NR8};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-cis-retinol + NADP(+) = 9-cis-retinal + H(+) + NADPH;
CC Xref=Rhea:RHEA:54916, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78272, ChEBI:CHEBI:78273;
CC Evidence={ECO:0000250|UniProtKB:Q96NR8};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 4-hydroxynonen-1-ol + NADP(+) = a 4-hydroxynonenal + H(+) +
CC NADPH; Xref=Rhea:RHEA:58336, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349, ChEBI:CHEBI:142593, ChEBI:CHEBI:142606;
CC Evidence={ECO:0000250|UniProtKB:Q96NR8};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-non-2-en-1-ol + NADP(+) = (E)-non-2-enal + H(+) + NADPH;
CC Xref=Rhea:RHEA:58332, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349, ChEBI:CHEBI:142592, ChEBI:CHEBI:142604;
CC Evidence={ECO:0000250|UniProtKB:Q96NR8};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(Z)-non-6-en-1-ol + NADP(+) = (Z)-non-6-enal + H(+) + NADPH;
CC Xref=Rhea:RHEA:58328, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349, ChEBI:CHEBI:142591, ChEBI:CHEBI:142603;
CC Evidence={ECO:0000250|UniProtKB:Q96NR8};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NADP(+) + nonan-1-ol = H(+) + NADPH + nonanal;
CC Xref=Rhea:RHEA:58380, ChEBI:CHEBI:15378, ChEBI:CHEBI:35986,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:84268;
CC Evidence={ECO:0000250|UniProtKB:Q96NR8};
CC -!- PATHWAY: Cofactor metabolism; retinol metabolism.
CC {ECO:0000250|UniProtKB:Q96NR8}.
CC -!- TISSUE SPECIFICITY: Expressed in the retina.
CC {ECO:0000269|PubMed:12226107}.
CC -!- MISCELLANEOUS: Shows clear specificity for the pro-S hydrogen on C4 of
CC NADPH and the pro-R hydrogen on C15 of retinols.
CC {ECO:0000250|UniProtKB:Q96NR8}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; AY115489; AAM51556.1; -; mRNA.
DR RefSeq; NP_899207.1; NM_183363.1.
DR AlphaFoldDB; P59837; -.
DR SMR; P59837; -.
DR STRING; 9913.ENSBTAP00000017058; -.
DR PaxDb; P59837; -.
DR PRIDE; P59837; -.
DR GeneID; 369021; -.
DR KEGG; bta:369021; -.
DR CTD; 145226; -.
DR eggNOG; KOG1208; Eukaryota.
DR InParanoid; P59837; -.
DR OrthoDB; 1032903at2759; -.
DR UniPathway; UPA00912; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0001917; C:photoreceptor inner segment; ISS:UniProtKB.
DR GO; GO:0102354; F:11-cis-retinol dehydrogenase activity; IEA:RHEA.
DR GO; GO:0004745; F:NAD-retinol dehydrogenase activity; ISS:UniProtKB.
DR GO; GO:0052650; F:NADP-retinol dehydrogenase activity; ISS:UniProtKB.
DR GO; GO:0110095; P:cellular detoxification of aldehyde; ISS:UniProtKB.
DR GO; GO:0042572; P:retinol metabolic process; ISS:UniProtKB.
DR GO; GO:0007601; P:visual perception; ISS:UniProtKB.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Lipid metabolism; NADP; Oxidoreductase; Reference proteome;
KW Sensory transduction; Vision.
FT CHAIN 1..316
FT /note="Retinol dehydrogenase 12"
FT /id="PRO_0000054765"
FT ACT_SITE 200
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 46..52
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 175
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 316 AA; 35171 MW; 2582630B2CA04185 CRC64;
MLVVLGLLTS FLSFLYVIAP SIRKFFAGGV CRTDVQLFGK VVVITGANTG IGKETARELA
RRGARVYIAC RDVLKGESAA SEIQADTKNS QVLVRKLDLS DTKSIRAFAE GFLAEEKQLH
ILINNAGVML CPYSKTADGF ETHLAVNHLG HFLLTHLLLG RLKESAPARV VNLSSVAHHL
GKIRFHDLQG DKYYNLGFAY CHSKLANVLF TRELAKRLKG TGVTTYAVHP GIVRSKLVRH
SFLLCLLWRL FSPFLKTTWE GAQTSLHCAL AEGLEPLSGK YFSDCKKTWV SPRARNNKTA
ERLWNVSCEL LGIRWE
