RDH7_MOUSE
ID RDH7_MOUSE Reviewed; 316 AA.
AC O88451;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Retinol dehydrogenase 7;
DE EC=1.1.1.105;
DE AltName: Full=Cis-retinol/3alpha-hydroxysterol short-chain dehydrogenase isozyme 2;
DE AltName: Full=Cis-retinol/androgen dehydrogenase type 2;
DE Short=CRAD-2;
GN Name=Rdh7; Synonyms=Crad2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=9651397; DOI=10.1074/jbc.273.28.17910;
RA Su J., Chai X., Kahn B., Napoli J.L.;
RT "cDNA cloning, tissue distribution, and substrate characteristics of a cis-
RT retinol/3alpha-hydroxysterol short-chain dehydrogenase isozyme.";
RL J. Biol. Chem. 273:17910-17916(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Ola; TISSUE=Liver;
RX PubMed=10876094; DOI=10.1016/s0378-1119(00)00194-3;
RA Tomita K., Sato M., Kajiwara K., Tanaka M., Tamiya G., Makino S.,
RA Tomizawa M., Mizutani A., Kuwano Y., Shiina T., Ishii H., Kimura M.;
RT "Gene structure and promoter for Crad2 encoding mouse cis-retinol/3-
RT hydroxysterol short-chain dehydrogenase isozyme.";
RL Gene 251:175-186(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Acts on androgens and retinols, i.e. has steroid 3-alpha- and
CC 17-beta-dehydrogenase and cis/trans-retinol catalytic activities.
CC {ECO:0000269|PubMed:9651397}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-retinol--[retinol-binding protein] + NAD(+) = all-
CC trans-retinal--[retinol-binding protein] + H(+) + NADH;
CC Xref=Rhea:RHEA:48488, Rhea:RHEA-COMP:14428, Rhea:RHEA-COMP:14430,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17336, ChEBI:CHEBI:17898,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:83228;
CC EC=1.1.1.105;
CC -!- PATHWAY: Cofactor metabolism; retinol metabolism.
CC -!- SUBCELLULAR LOCATION: Microsome {ECO:0000250}. Endoplasmic reticulum
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Highly expressed in liver. Also expressed in lung,
CC eye, kidney, and brain. {ECO:0000269|PubMed:9651397}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; AF056194; AAC40159.1; -; mRNA.
DR EMBL; AB015165; BAB03717.1; -; mRNA.
DR EMBL; AB032058; BAB03718.1; -; mRNA.
DR EMBL; AB032057; BAB03719.1; -; Genomic_DNA.
DR EMBL; BC024603; AAH24603.1; -; mRNA.
DR EMBL; BC092255; AAH92255.1; -; mRNA.
DR EMBL; BC093514; AAH93514.1; -; mRNA.
DR CCDS; CCDS24255.1; -.
DR RefSeq; NP_001144221.1; NM_001150749.1.
DR RefSeq; NP_059501.1; NM_017473.4.
DR AlphaFoldDB; O88451; -.
DR SMR; O88451; -.
DR IntAct; O88451; 1.
DR STRING; 10090.ENSMUSP00000039252; -.
DR iPTMnet; O88451; -.
DR PhosphoSitePlus; O88451; -.
DR SwissPalm; O88451; -.
DR jPOST; O88451; -.
DR MaxQB; O88451; -.
DR PaxDb; O88451; -.
DR PeptideAtlas; O88451; -.
DR PRIDE; O88451; -.
DR ProteomicsDB; 253192; -.
DR DNASU; 54150; -.
DR Ensembl; ENSMUST00000047199; ENSMUSP00000039252; ENSMUSG00000040134.
DR GeneID; 54150; -.
DR KEGG; mmu:54150; -.
DR UCSC; uc007hkv.2; mouse.
DR CTD; 54150; -.
DR MGI; MGI:1860517; Rdh7.
DR VEuPathDB; HostDB:ENSMUSG00000040134; -.
DR eggNOG; KOG1610; Eukaryota.
DR GeneTree; ENSGT00940000154118; -.
DR HOGENOM; CLU_010194_2_0_1; -.
DR InParanoid; O88451; -.
DR OMA; FRWYQER; -.
DR OrthoDB; 942985at2759; -.
DR PhylomeDB; O88451; -.
DR TreeFam; TF325617; -.
DR BRENDA; 1.1.1.315; 3474.
DR UniPathway; UPA00912; -.
DR BioGRID-ORCS; 54150; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Rdh7; mouse.
DR PRO; PR:O88451; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; O88451; protein.
DR Bgee; ENSMUSG00000040134; Expressed in left lobe of liver and 40 other tissues.
DR Genevisible; O88451; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0047044; F:androstan-3-alpha,17-beta-diol dehydrogenase activity; ISO:MGI.
DR GO; GO:0047023; F:androsterone dehydrogenase activity; ISO:MGI.
DR GO; GO:0003824; F:catalytic activity; ISS:MGI.
DR GO; GO:0004745; F:NAD-retinol dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016229; F:steroid dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0042572; P:retinol metabolic process; IBA:GO_Central.
DR GO; GO:0008202; P:steroid metabolic process; ISO:MGI.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Microsome; NAD; NADP; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..316
FT /note="Retinol dehydrogenase 7"
FT /id="PRO_0000054761"
FT ACT_SITE 175
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 33..57
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 163
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 316 AA; 35660 MW; 0BFB9A1C2DDE3C17 CRC64;
MWLYLVALVG LWTLLRFFRE RQVVSHLQDK YVFITGCDSG FGNLLARQLD RRGMRVLAAC
LTEKGAEQLR NKTSDRLETV ILDVTKTESI VAATQWVKER VGNRGLWGLV NNAGICVFAI
NEWLKKEDFA NILDVNLLGM IEVTLSMLPL VRKARGRVVN ISSSMGRVSL CGGGYCISKY
GVEAFSDSLR REISYFGVKV AIIEPGGFRT NVSNYERLSH SIEKLWDQTS SEVKEVYDKN
FLDSYIKAIQ SLTDTCSDDL SVVTDCMEHA LTACHPRTRY SAGWDAKLFY LPLSYMPTFL
VDAMLYWSSV KPAQAL
