RDL1_ARATH
ID RDL1_ARATH Reviewed; 376 AA.
AC Q94B08; O23195; Q0WPN3;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Germination-specific cysteine protease 1 {ECO:0000303|Ref.1};
DE EC=3.4.22.-;
DE AltName: Full=RD21A-like protease 1 {ECO:0000305};
DE Flags: Precursor;
GN Name=GCP1 {ECO:0000303|Ref.1}; Synonyms=RDL1 {ECO:0000305};
GN OrderedLocusNames=At4g36880; ORFNames=C7A10.480;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Pritchard S.L., Graham I.A.;
RT "A germination-specific cysteine proteinase (GCP1) from Arabidopsis
RT thaliana.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION, AND SEQUENCE REVISION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP INDUCTION BY GIBBERELLIN.
RX PubMed=17384169; DOI=10.1105/tpc.106.048009;
RA Ariizumi T., Steber C.M.;
RT "Seed germination of GA-insensitive sleepy1 mutants does not require RGL2
RT protein disappearance in Arabidopsis.";
RL Plant Cell 19:791-804(2007).
CC -!- FUNCTION: Probable thiol protease. {ECO:0000250|UniProtKB:P43297}.
CC -!- DEVELOPMENTAL STAGE: Germination specific. {ECO:0000305|Ref.1}.
CC -!- INDUCTION: By gibberellin. {ECO:0000269|PubMed:17384169}.
CC -!- SIMILARITY: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089,
CC ECO:0000255|PROSITE-ProRule:PRU10090}.
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DR EMBL; AY043294; AAK92229.1; -; mRNA.
DR EMBL; Z99707; CAB16767.1; -; Genomic_DNA.
DR EMBL; AL161590; CAB80354.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86713.1; -; Genomic_DNA.
DR EMBL; AK229031; BAF00916.1; -; mRNA.
DR PIR; E85435; E85435.
DR RefSeq; NP_195406.2; NM_119852.5.
DR AlphaFoldDB; Q94B08; -.
DR SMR; Q94B08; -.
DR STRING; 3702.AT4G36880.1; -.
DR MEROPS; C01.021; -.
DR PaxDb; Q94B08; -.
DR PeptideAtlas; Q94B08; -.
DR PRIDE; Q94B08; -.
DR ProteomicsDB; 236432; -.
DR GeneID; 829841; -.
DR KEGG; ath:AT4G36880; -.
DR Araport; AT4G36880; -.
DR eggNOG; KOG1543; Eukaryota.
DR HOGENOM; CLU_012184_1_0_1; -.
DR InParanoid; Q94B08; -.
DR OrthoDB; 1275401at2759; -.
DR PhylomeDB; Q94B08; -.
DR PRO; PR:Q94B08; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q94B08; baseline and differential.
DR Genevisible; Q94B08; AT.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0051603; P:proteolysis involved in protein catabolic process; IBA:GO_Central.
DR CDD; cd02248; Peptidase_C1A; 1.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR025661; Pept_asp_AS.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR025660; Pept_his_AS.
DR InterPro; IPR000668; Peptidase_C1A_C.
DR InterPro; IPR039417; Peptidase_C1A_papain-like.
DR InterPro; IPR013201; Prot_inhib_I29.
DR Pfam; PF08246; Inhibitor_I29; 1.
DR Pfam; PF00112; Peptidase_C1; 1.
DR PRINTS; PR00705; PAPAIN.
DR SMART; SM00848; Inhibitor_I29; 1.
DR SMART; SM00645; Pept_C1; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS00640; THIOL_PROTEASE_ASN; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR PROSITE; PS00639; THIOL_PROTEASE_HIS; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Germination; Glycoprotein; Hydrolase; Protease;
KW Reference proteome; Signal; Thiol protease; Zymogen.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..144
FT /note="Activation peptide"
FT /evidence="ECO:0000305"
FT /id="PRO_0000026404"
FT CHAIN 145..376
FT /note="Germination-specific cysteine protease 1"
FT /id="PRO_0000026405"
FT ACT_SITE 169
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10088"
FT ACT_SITE 305
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10089"
FT ACT_SITE 325
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10090"
FT CARBOHYD 93
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 166..208
FT /evidence="ECO:0000250|UniProtKB:P84346"
FT DISULFID 200..241
FT /evidence="ECO:0000250|UniProtKB:P84346"
FT DISULFID 299..351
FT /evidence="ECO:0000250|UniProtKB:P84346"
FT CONFLICT 90
FT /note="D -> N (in Ref. 1; AAK92229, 4; AEE86713 and 5;
FT BAF00916)"
FT /evidence="ECO:0000305"
FT CONFLICT 277
FT /note="S -> R (in Ref. 5; BAF00916)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 376 AA; 41640 MW; 17333DFCD0BC4C8E CRC64;
MAPSTKVLSL LLLYVVVSLA SGDESIINDH LQLPSDGKWR TDEEVRSIYL QWSAEHGKTN
NNNNGIINDQ DKRFNIFKDN LRFIDLHNED NKNATYKLGL TKFTDLTNDE YRKLYLGART
EPARRIAKAK NVNQKYSAAV NGKEVPETVD WRQKGAVNPI KDQGTCGSCW AFSTTAAVEG
INKIVTGELI SLSEQELVDC DKSYNQGCNG GLMDYAFQFI MKNGGLNTEK DYPYRGFGGK
CNSFLKNSRV VSIDGYEDVP TKDETALKKA ISYQPVSVAI EAGGRIFQHY QSGIFTGSCG
TNLDHAVVAV GYGSENGVDY WIVRNSWGPR WGEEGYIRME RNLAASKSGK CGIAVEASYP
VKYSPNPVRG NTISSV
