ID   RDPO_TETOB              Reviewed;         608 AA.
AC   P19593;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Probable reverse transcriptase;
DE            EC=2.7.7.49;
OS   Tetradesmus obliquus (Green alga) (Acutodesmus obliquus).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Sphaeropleales; Scenedesmaceae; Tetradesmus.
OX   NCBI_TaxID=3088;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=KS3/2;
RX   PubMed=2476655; DOI=10.1007/bf00331276;
RA   Kueck U.;
RT   "The intron of a plastid gene from a green alga contains an open reading
RT   frame for a reverse transcriptase-like enzyme.";
RL   Mol. Gen. Genet. 218:257-265(1989).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU00405};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
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DR   AlphaFoldDB; P19593; -.
DR   SMR; P19593; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   CDD; cd00085; HNHc; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR002711; HNH.
DR   InterPro; IPR003615; HNH_nuc.
DR   InterPro; IPR013597; Mat_intron_G2.
DR   InterPro; IPR000477; RT_dom.
DR   InterPro; IPR025960; RVT_N.
DR   Pfam; PF08388; GIIM; 1.
DR   Pfam; PF01844; HNH; 1.
DR   Pfam; PF00078; RVT_1; 1.
DR   Pfam; PF13655; RVT_N; 1.
DR   SMART; SM00507; HNHc; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50878; RT_POL; 1.
PE   4: Predicted;
KW   Chloroplast; Nucleotidyltransferase; Plastid; RNA-directed DNA polymerase;
KW   Transferase.
FT   CHAIN           1..608
FT                   /note="Probable reverse transcriptase"
FT                   /id="PRO_0000217416"
FT   DOMAIN          105..343
FT                   /note="Reverse transcriptase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT   DOMAIN          525..571
FT                   /note="HNH"
SQ   SEQUENCE   608 AA;  69557 MW;  3AE4E604AA140009 CRC64;
     MKRIKLKYRK LKRQQFIKVE KFNIGIDMVE CHRWLENKQE SIACAKREGN IVLVEKLAQE
     IVNSSFGRAV AVQTVASSKG SRSPGLSRES FKTNKNYVAM MATLEQITSN PHKYKATPLS
     RIYIPKRDGS ARPLSIPSYT DRCLQALYKL AIEPMAEEVA DLSSYGFRPM RNVSWAVGRV
     LNGLNNPLAN YQYVVEIDIK GCVDNINHQF ISQVTPFIPK KILWAWLKCG YIERNSNTLQ
     PTTTGVPQGG IISPLIMNLT LDGLEFHIYK KIQKSSSQSK GNTYCRYADD MVILTTTEET
     ALIALPAVKE FLAVRGLEVK LAKTTIKNII NDRNGFEFLS FRFRKVYRRN RKRLTSQVGI
     PISAIKNFRK NIKAISKTRK SLDTINDEIN AVFRDCGYYY RFAHTSGYVF SSLGYWLWKQ
     FYKHCYKRTK DKFDKANHTK INEIVLSSYF KPIGSGLSTK PFVIDKKGKP HSLFSIRSIE
     YCPPTYTDSA RNAFIFEDSV TLTKVNMRSK SSWKRVILEK WGPCCGLCRK NLEINSIPYE
     LHHILPKRFG GKDTPNNMVL LCKSPCHQLV SSSIQKADVS EIQNYISLGI LEIPMDYLEN
     LKTSQSSY