ID   RDR4_ARATH              Reviewed;         927 AA.
AC   O82189;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Probable RNA-dependent RNA polymerase 4;
DE            Short=AtRDRP4;
DE            EC=2.7.7.48;
DE   AltName: Full=RNA-directed RNA polymerase 4;
GN   Name=RDR4; Synonyms=RDRP4; OrderedLocusNames=At2g19920; ORFNames=F6F22.5;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: Probably involved in the RNA silencing pathway and required
CC       for the generation of small interfering RNAs (siRNAs). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48;
CC   -!- SIMILARITY: Belongs to the RdRP family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC62124.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC005169; AAC62124.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC06943.1; -; Genomic_DNA.
DR   PIR; G84582; G84582.
DR   RefSeq; NP_179582.2; NM_127550.3.
DR   AlphaFoldDB; O82189; -.
DR   BioGRID; 1866; 1.
DR   STRING; 3702.AT2G19920.1; -.
DR   PaxDb; O82189; -.
DR   PRIDE; O82189; -.
DR   EnsemblPlants; AT2G19920.1; AT2G19920.1; AT2G19920.
DR   GeneID; 816511; -.
DR   Gramene; AT2G19920.1; AT2G19920.1; AT2G19920.
DR   KEGG; ath:AT2G19920; -.
DR   Araport; AT2G19920; -.
DR   TAIR; locus:2052030; AT2G19920.
DR   eggNOG; KOG0988; Eukaryota.
DR   HOGENOM; CLU_008367_0_0_1; -.
DR   InParanoid; O82189; -.
DR   PhylomeDB; O82189; -.
DR   PRO; PR:O82189; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O82189; baseline and differential.
DR   GO; GO:0031380; C:nuclear RNA-directed RNA polymerase complex; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0030422; P:siRNA processing; IBA:GO_Central.
DR   InterPro; IPR007855; RNA-dep_RNA_pol_euk-typ.
DR   PANTHER; PTHR23079; PTHR23079; 1.
DR   Pfam; PF05183; RdRP; 1.
PE   3: Inferred from homology;
KW   Nucleotidyltransferase; Reference proteome; RNA-binding;
KW   RNA-directed RNA polymerase; RNA-mediated gene silencing; Transferase.
FT   CHAIN           1..927
FT                   /note="Probable RNA-dependent RNA polymerase 4"
FT                   /id="PRO_0000404675"
FT   REGION          98..135
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        117..135
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   927 AA;  105180 MW;  DA5DAF2CCE2188D4 CRC64;
     MMTTTMDYNS SDQGFSWSEI ALLGSVETML EKVYGKHNHH PPIKVETRRR LSSISEELAL
     ETLRKVLNMP NVKTLDGIII YFLNDAVTVD GSPRLWSGES PVQFPRTPGK KSCRASQAEV
     SLDREDPSPK FLRGDENGES KHISLLLALG ELEFKKAFLL LTYLGGKSLG EVISGDEIRQ
     WKDLPMVAYE RAVWFKLGQN EERMQLESDS GKTHYYQCHV APDGSYRLKG YFLENTGTHL
     HKVLGDDNVL TVRFDQLPKE STYCDNPYSK YKEIAKNGIM VGLRRYQFFV FKDGGKAEKK
     KRNSTKQVKC YFIRTGSTAS SDMENPYILS GMSIHEARMH FMHVHTLPSP ANYMARFSLI
     LSKTKKLEVD MTEITVMQID DIHCHDQSNN DVLDKNGKPR IHSDGTGYIS EDLARMCPLN
     IFKGKSMRSN NIQGTCVQEP PLLIQIRMFN DGSAVKGIFL LNKNLPPQTV QVRPSMIKVY
     KDKNLSNFST FNSLEVVTTS NPPKRAKLSK NLVALLSYGG VPNDFFLDIL LNTLEKKKTI
     FFKVRAAGKA ALHYGNMDDK NALQMIMAGI PLDEPYLKHY LSKLLKLEKD DLKAGKLPID
     ESYYLMGTVD PTGELKEDEV SGLAKSQDVL VYRNPGLHFG DIHILKATYV KSLEQYVGNS
     KYGVFFPQKG PRSLGDEIAG GDFDGDMYFI SRNPKLLEHY KPSEPWVSSS PRSKIYTGRQ
     PSELSPEQLE EELFKIFLKT GFSPSSVIGQ AADSWLAIMD RFLTLGDENV KEKAEMKKKM
     LKLTDIYYDA IDAPKTGTEV NLPLDVKVDL FPHYMERNKT FKSTSILGLI FDTVDFHNAE
     DTTPSGISKL QCFEDEPVSE FDMEKCKLWH KDYRKEMCQA MNSDDDDSCN EVIQKYKQEF
     YSAAGFKESK KILEELYPKA LALYNVT