RDRP_ACLSA
ID RDRP_ACLSA Reviewed; 1885 AA.
AC P54891;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=RNA-directed RNA polymerase;
DE EC=2.7.7.48;
DE AltName: Full=216.5 kDa protein;
DE AltName: Full=ORF1 protein;
DE AltName: Full=RNA replicase;
OS Apple chlorotic leaf spot virus (isolate apple) (ACLSV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Betaflexiviridae; Trivirinae; Trichovirus.
OX NCBI_TaxID=73472;
OH NCBI_TaxID=23159; Crataegus (hawthorn).
OH NCBI_TaxID=36610; Cydonia oblonga (Quince) (Pyrus cydonia).
OH NCBI_TaxID=3752; Malus sylvestris.
OH NCBI_TaxID=36596; Prunus armeniaca (Apricot) (Armeniaca vulgaris).
OH NCBI_TaxID=3758; Prunus domestica (Garden plum).
OH NCBI_TaxID=3760; Prunus persica (Peach) (Amygdalus persica).
OH NCBI_TaxID=114937; Prunus spinosa (Blackthorn) (Prunus domestica var. spinosa).
OH NCBI_TaxID=23211; Pyrus communis (Pear) (Pyrus domestica).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8376968; DOI=10.1099/0022-1317-74-9-1927;
RA Sato K., Yoshikawa N., Takahashi T.;
RT "Complete nucleotide sequence of the genome of an apple isolate of apple
RT chlorotic leaf spot virus.";
RL J. Gen. Virol. 74:1927-1931(1993).
CC -!- FUNCTION: Involved in viral RNA replication. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
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DR EMBL; D14996; BAA03641.1; -; Genomic_RNA.
DR PIR; JQ2183; JQ2183.
DR PRIDE; P54891; -.
DR Proteomes; UP000000397; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR Gene3D; 2.60.120.590; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR008744; RNA-dir_pol_ACLSV.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR Pfam; PF05413; Peptidase_C34; 1.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 4: Predicted;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding;
KW Nucleotidyltransferase; RNA-directed RNA polymerase; Transferase;
KW Viral RNA replication.
FT CHAIN 1..1885
FT /note="RNA-directed RNA polymerase"
FT /id="PRO_0000222541"
FT DOMAIN 63..239
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT DOMAIN 748..836
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT DOMAIN 1027..1192
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 1193..1337
FT /note="(+)RNA virus helicase C-terminal"
FT DOMAIN 1634..1741
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT REGION 607..639
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 607..635
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 1060..1067
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1885 AA; 216507 MW; 7A3FD14D0D8BF90E CRC64;
MAFSYRTPQE ELLSRLPQSQ QEAISGFQYE RFQKEEEKKV ENFSFYLPEK TREWFTKSGV
YLSPFAYVNH SHPGCKTLEN HLLFNVVASY ISKYSYVACL SIKSNKMSKM ERLGSNSVKT
YDILNRLVTA KDKARYGPLA RPERSPCPKK TNIFIHDEIH YWSRDQLETF LQVHRPKNLW
ATLVFPPEIL AGYKSSVLPF LYQFEIHGKD LVYMPDGVRS ESYTQPLENG FLLSSSSILV
RNKATGVEIR YQVSLVYSLG SHHLFHIYPT EDLMKEEVRR FGPYDLFDVG SLFVKPVRVP
IQDFPLSVFK KIFIYLSSLK KPDVQSAVAK LRQLSDADIS IESVFMVQEF ASRVEKNGVG
SWSCSFWECM KDWFFDKLPY REVLEKIGLA NDFTRRLMKI KPLAFDIHTT DRPLTVRMVI
DQIWGERQSS CDDVPNIIFY GRKEWLEHGL LPKVKKGLAK LVPGRETGGS DYPEEIYSDL
LSSTSIWRSY DENLRHRKAS PIVILKSEKA YSEAPGFSSN CISLCSTPFG EVIERTPFEV
ERERKKRELS FGCLDFHIRK MKVKDASELS IKLDEQNKEG LRRQKRKEKA KKTRMIPVHL
LELGSDQKEK NLGQEASKGK GIEQEERRKS DEAKFDSGPS GVCSIKAENP VDAQHIAEPV
PCLKLNDLIG KEKICSSGLI KTVGNDYLTL ARQIEDMPLA QLKNRKAAYF CIDYPMVYFH
DKISYPTFEA TGEIKQIIMR ARDKWGANFN SALIQVYNDG CRLPLHSDNE ECYDDDEILT
INVVGDAKFH TTCHGEIIDL RQGDEILMPG GYQKMNKHAV EVASEGRTSV TLRVHKRDFS
FESKLRFIKG KFDCLFVSIA EIIHKKPEEV MMFIPHIVDR CVSNRGCSLD DARAICEKYE
IKIECEGDCG LVECGTIGLS VGRMLLRGNH FTVASVRRSS MDSLANSSKE LKSNGVLDHV
MFNFHKRLRS VEPDLTVEEI KVDSSRAGKL LKSLMDGMTG IVSHNSTHEG WRMIKGINST
SEMRSFMSMI KGKSEETRGD LFDRVQELNF MKVKIYGIFG FAGSGKSHAI QNLIQTEFKG
SQGVMVICPR RFLAKDWSEK GVDEKDIKTF ESALKSDVKG KRLFILDEIS LLPKGFTDLL
MLKMHMEGIL KKSTIVCIGD PLQAGYFCPK DDNYLSREGE IKRLFKDGVN YKWYSYRINK
FIAKKLAIET MNDFIGIDEQ NLIYKDMPSA HHFMENKGNH IEVILVPSMI EKELYSNYGN
VMTFGESQGL TFNCGVIVLS EEAKLCSDAH IMVAITRFRR GFCFALGSKG SKEDYMRSMK
SGLLQRMCSG IGASKEFILG SSSVKLILSE KDVSKGAGVD EMDREARLEG DVWLKSMIYL
GKRYHIVEPL GQVIKLTENA IKCHIPVCSS QTLGPELDNI QAREFREFKG KNGWSNQFRE
EAGPNWKHPY RVNQAMSYEA IYPRHRMDDD LTFLAAIKKR LRFDNVANNY AKFKAAESRG
KYLAKVFLRH VPIKCGRDQR LLDQCRQEFE ETKLSKSAAT IGAHSQRSDS DWPLDKIFLF
MKSQLCTKFE KRFTEAKAGQ TLACFPHKIL VEFSPWCRYT EKVLTANLPD NYYIHQRKNF
SELEDFAKRF SNGSMCVESD YTAFDVSQDH TILAFEVELL KHFGWDDKVL QSYIKMKCTL
GCRLGGFAIM RFTGEFSTFL FNTLANMVFT FCRYEVPDGT PICFAGDDMC ALRNLREIDT
HECILSKLSL KAKVNRTKVP MFCGWRLCFD GLIKEPCLIY ERLQVAIENG RLMDVIDSYF
LEFSFAYKLG ERLYSHLEIE QLNYHQVLTR FFIRNKHLLR GDSRHNISEL EWLSDEDSDD
DKGSQIEDRR RGYSNCWGEK LQNLF
