RDRP_ACLSP
ID RDRP_ACLSP Reviewed; 1884 AA.
AC P27738;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=RNA-directed RNA polymerase;
DE EC=2.7.7.48;
DE AltName: Full=216.5 kDa protein;
DE AltName: Full=ORF1 protein;
DE AltName: Full=RNA replicase;
OS Apple chlorotic leaf spot virus (isolate plum P863) (ACLSV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Betaflexiviridae; Trivirinae; Trichovirus.
OX NCBI_TaxID=73473;
OH NCBI_TaxID=23159; Crataegus (hawthorn).
OH NCBI_TaxID=36610; Cydonia oblonga (Quince) (Pyrus cydonia).
OH NCBI_TaxID=3752; Malus sylvestris.
OH NCBI_TaxID=36596; Prunus armeniaca (Apricot) (Armeniaca vulgaris).
OH NCBI_TaxID=3758; Prunus domestica (Garden plum).
OH NCBI_TaxID=3760; Prunus persica (Peach) (Amygdalus persica).
OH NCBI_TaxID=114937; Prunus spinosa (Blackthorn) (Prunus domestica var. spinosa).
OH NCBI_TaxID=23211; Pyrus communis (Pear) (Pyrus domestica).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2219716; DOI=10.1016/0042-6822(90)90279-z;
RA German S., Candresse T., Lanneau M., Huet J.-C., Pernollet J.-C., Dunez J.;
RT "Nucleotide sequence and genomic organization of apple chlorotic leaf spot
RT closterovirus.";
RL Virology 179:104-112(1990).
CC -!- FUNCTION: Involved in viral RNA replication. {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
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DR EMBL; M58152; AAA42587.1; -; Genomic_RNA.
DR PIR; A45353; A45353.
DR RefSeq; NP_040551.1; NC_001409.1.
DR PRIDE; P27738; -.
DR GeneID; 1493999; -.
DR KEGG; vg:1493999; -.
DR Proteomes; UP000007025; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR Gene3D; 2.60.120.590; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR008744; RNA-dir_pol_ACLSV.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR Pfam; PF05413; Peptidase_C34; 1.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 4: Predicted;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding;
KW Nucleotidyltransferase; Reference proteome; RNA-directed RNA polymerase;
KW Transferase; Viral RNA replication.
FT CHAIN 1..1884
FT /note="RNA-directed RNA polymerase"
FT /id="PRO_0000222542"
FT DOMAIN 63..232
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT DOMAIN 747..835
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT DOMAIN 1032..1191
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 1192..1336
FT /note="(+)RNA virus helicase C-terminal"
FT DOMAIN 1633..1740
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT REGION 602..648
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 609..648
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 1059..1066
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VARIANT 347
FT /note="I -> V"
FT VARIANT 591
FT /note="A -> P"
FT VARIANT 594
FT /note="S -> L"
FT VARIANT 666
FT /note="D -> R"
FT VARIANT 1598
FT /note="Y -> H"
FT VARIANT 1623
FT /note="E -> K"
SQ SEQUENCE 1884 AA; 216562 MW; 966A9F5DBA300258 CRC64;
MAFSYRTPQE ELLSRLPQSQ QEVISGFQYE RIQKEEEKKV ENFSFYLPEK TREWFTKSGV
YLSPFAYVNH SHPGCKTLEN HLLFNVVASY ISKYSYVACL SIKSNKMSKM ERLGPNSVKT
YDILNRLVTA KDKARYGPLA KPERSPCPKK TNIFIHDEIH YWSRDQLETF LQVHRPKNLW
ATLVFPPEIL AGYKSSVLPF LYQFEIHGKD LVYMPDGVRS ESYTQPLENG FLLSSSSIII
KNRVTGVEIR YQVSLVYSLG SHHLFHIYPA EDLMKEEVRR FGPYDLFDVG SLFVKPVRVP
IQDFPLSVFK KIFIYMSSLK KPDVQSAVAK LRQLSDADIS IESVFMIQEF ASRIEKNGVE
SWSCSFWGCM KDWFFDKLPY REVLEKIGLA DDFTRRLMKI KPLAFDIHTT DRPLTVRMII
DQIWEERLSS FDNISNIVFY GRREWLNNGV LPKVKKKGLA KLIPGREVDS HNYPREIYSD
LLSSTSIWRS YDDDFRHRSA SPLVILRSNR AYSEAAKFSS NCLSLCAAPC DEVIARTPFE
LNHRREKKEL SLKCLDFHIK KMKVKNVLEL EVKLRERNTR ISLSKKGVKK AGRSRMIPVH
LLKPTCGEGN GKPEERNKEE AKIPMNEGTS KEEKGSEPPH SEVKREGVRL DEQHISEPLL
SFKLDDFVGR EKLCSAGLIK TVGNDYLLLA RQIECMPLSQ LRGKKAAYFC LDFPMVYFHD
KVSYPTFEAT GEIRHVMMKA RSKWGIDFNS ALIQVYNDGC RLPLHSDNEE CYDDDGILTI
NVVGDAKFHT TCHDEVIDLK QGNEILMPAG YQKKNRHAVE VASEGRTSVT LRVHKRDFSF
ESKLRFIKGK FDCLFVSVAE IIHKKPEEIM MFIPHIMDRC VSNRGCSLDD AKAICEKYEI
KIECEGDCGL VECGTSGLSI GRMLLRGNHF SVASVRRSSM DSLANSSKEI KSDGVLDHVT
FNFHKRLKLV EPDLTNADIK VDSSRAGKLL KSLMDGMTGI VSHNSTHEGW RMIKGINSTS
EMRSFMNMVR GQIEEPKSDL FDKVQELNFM KVKIYGIFGF AGSGKSHAIQ NLIQTEFKGS
QGIMVICPRR FLAKDWSEKG VDEKDIKTFE SALKSDVKGK RLFILDEISL LPKGFTDLLM
LKMHMEGILK KSTIVCIGDP LQAGYFCPKD DNYLSREGEI KRLFKGGVNY KWYSYRINKF
IAKKLAIETM NDFIGIDEQS SIYKDMPSAH HFMEKKGNHI EVILVASMVE KELYSNYGNV
MTFGESQGLT FNCGVIVLSE EAKLCSDAHI MVAITRFRRG FCFALGSKGS KEDYMRSMKS
GLLQRICSGV GASKEFILGS SSVNLILSEK DIAKGAGIDE MDREARLEGD VWLKSMIYLG
KRYHMVEPLG QVIKLTDDAI KCHIPVCSSQ TLGPELDNIQ AREYREFKGK NGWSNQFREE
AGPNWKFPYK VNQAMSYEAV YPRHKMDDDL TFLAAIKKRL RFDNVANNYA KFKAAESRGK
YLTKIFLKHV PIKCGRDQRL LDQCRQEFEE TKLSKSAATI GAHSQRSDSD WPLDKIFLFM
KSQLCTKFEK RFTEAKAGQT LACFPHKILV EFSPWCRYTE KVLTANLPDN YYIHQRKNFS
ELEDFARRFS NGSICVESDY TAFDVSQDHT ILAFEVELLR HFGWDDRVLQ SYIKMKCTLG
CRLGGFAIMR FTGEFSTFLF NTLANMVFTF CRYEVPDGTP ICFAGDDMCA LRNLREIDTH
EFILSKLSLK AKVNRTKVPM FCGWRLCCDG LIKEPCLIYE RLQVAIENGR LMDVIDSYFL
EFSFAYKLGE RLYSHLEIEQ LNYHQVLTRF FIRNKHLLRG DSRHNISELE WLSDEDGDND
KGSQIEDRRR GYSNCWGEKL QNLF
