ID   RDRP_AHNNV              Reviewed;         981 AA.
AC   Q9DIC5;
DT   16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   29-SEP-2021, entry version 56.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            Short=RdRp;
DE            EC=2.7.7.48;
DE   AltName: Full=RNA replicase;
DE            Short=Protein A;
OS   Atlantic halibut nervous necrosis virus (AhNNV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes;
OC   Nodamuvirales; Nodaviridae; Betanodavirus.
OX   NCBI_TaxID=99486;
OH   NCBI_TaxID=8267; Hippoglossus hippoglossus (Atlantic halibut) (Pleuronectes hippoglossus).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=AH99NorA;
RA   Sommerset I., Nerland A.H.;
RT   "The sequence of RNA1 from Atlantic halibut nervous necrosis virus
RT   (AHNNV).";
RL   Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 2 RNA segments, RNA1 AND RNA2. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SIMILARITY: Belongs to the nodaviridae RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; AJ401165; CAC17792.1; -; Genomic_RNA.
DR   Proteomes; UP000272105; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043647; Noda_Vmethyltr_dom.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF19222; Noda_Vmethyltr; 1.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA-directed RNA polymerase;
KW   Transferase; Viral RNA replication.
FT   CHAIN           1..981
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000222443"
FT   DOMAIN          579..701
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          879..981
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        880..896
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        917..943
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   981 AA;  110328 MW;  6587D72D676FEA50 CRC64;
     MRSYEFHLAR MSGATLCVVT GYRLLTSKWL ADRIEDYRQR VIADRKKIIR DAAMIRTSVQ
     KQMELVRISV RKGHSHQEAA TERNSATETM LSVVEKSGYE PYIISPSPRE AEYHGSRQFY
     SLADFRQDYR RDEITDRHII VMTDVDYYVD MNELIGLGVP VLMYTFQPST VSGEVKDGYF
     TITDDSVHYR VAGGKDVRHR IWNYNQDTMY TVSRPTGFWE NLKRILRDIT GITALCGFLY
     NKLGMAPFGD PVTMFTVDQF KMGEHRNIVS IVPFATCRSN LLKISEYGAE LEYMRYQQRN
     NIANFNAVTY ISEEGPLISL GLEGNFASVQ LPLQDFENVR TAYELSKTNN LSDTVRRSGR
     PCKEAAIIHK CLQAECVLAS EVVHKPGDLA RHYQAVGSLY DVDPAEQGKC YAREYAPGPL
     TQTAVFPNES RSNERATIDG RIAGPQAKAK SREHITPRMR KVARDFVRHL VPEAGLGRPY
     PLTYVEEQQS KPLQRARNDA NRYHDEFTMI VKAFQKKEAY NAPNYPRNIS TVPHNQNVKL
     SSYTYAFKAS TLQHVPWYMP THTPAEIADA VQNLAASTTE LVETDYSKFD GTFLRFMREC
     VEFAIYKRWV HLDHLAELSQ LLANELQAPA VTRLGLKYDP DCSRLSGSAL TTDGNSIANA
     FVSYLANRLA GMDDDEAWSW IGIVYGDDGL RSGNVSDTLL TDTASSLGFD LKIVNRAPRG
     SPVTFLARVY LDPWSSPASV QSPFRTLLKL HTTCDTQSEI EDIGWAKTQA YLVTDCKTPF
     IGHWCRAYQR NCTARVVQYA DYNDIPFWVK NDDHLGNSWP QSDSVDWNDV VANELGLTTA
     ELLKHIALLE AYTGPTSGLP RLTTSIDLEP KMSVALDGEV QAGPSQQQTD KDGTNPTGDR
     SAPRRARTAL PGVDGHTRCT RRSDRGPGER DANVRDKRPR RSMPPSRSVS SVPPPSSSGG
     GADGDRVEGA ARPRRQRRTP V