ID   RDRP_AMVLE              Reviewed;         790 AA.
AC   P03593;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Alfalfa mosaic virus (strain 425 / isolate Leiden).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Alfamovirus.
OX   NCBI_TaxID=12322;
OH   NCBI_TaxID=4045; Apium graveolens (Celery).
OH   NCBI_TaxID=83862; Astragalus glycyphyllos (Wild liquorice).
OH   NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH   NCBI_TaxID=41386; Caryopteris incana.
OH   NCBI_TaxID=3827; Cicer arietinum (Chickpea) (Garbanzo).
OH   NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH   NCBI_TaxID=35936; Lablab purpureus (Hyacinth bean) (Dolichos lablab).
OH   NCBI_TaxID=4236; Lactuca sativa (Garden lettuce).
OH   NCBI_TaxID=3864; Lens culinaris (Lentil) (Cicer lens).
OH   NCBI_TaxID=3869; Lupinus.
OH   NCBI_TaxID=145753; Malva parviflora (Little mallow) (Cheeseweed mallow).
OH   NCBI_TaxID=3879; Medicago sativa (Alfalfa).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH   NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH   NCBI_TaxID=23113; Philadelphus.
OH   NCBI_TaxID=3888; Pisum sativum (Garden pea).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
OH   NCBI_TaxID=157662; Teramnus repens.
OH   NCBI_TaxID=60916; Trifolium incarnatum (Crimson clover).
OH   NCBI_TaxID=85293; Viburnum opulus (High-bush cranberry).
OH   NCBI_TaxID=3916; Vigna radiata var. radiata (Mung bean) (Phaseolus aureus).
OH   NCBI_TaxID=3917; Vigna unguiculata (Cowpea).
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=6304618; DOI=10.1093/nar/11.10.3019;
RA   Cornelissen B.J.C., Brederode F.T., Veeneman G.H., van Boom J.H., Bol J.F.;
RT   "Complete nucleotide sequence of alfalfa mosaic virus RNA 2.";
RL   Nucleic Acids Res. 11:3019-3025(1983).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the bromoviridae 2a family. {ECO:0000305}.
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DR   EMBL; X01572; CAA25728.1; -; Unassigned_RNA.
DR   PIR; A04201; WMFM9.
DR   RefSeq; YP_053235.1; NC_002024.2.
DR   GeneID; 962668; -.
DR   KEGG; vg:962668; -.
DR   Proteomes; UP000000358; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..790
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000083267"
FT   DOMAIN          524..639
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          771..790
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   790 AA;  89855 MW;  B435C9EB9AA853C2 CRC64;
     MFTLLRCLGF GVNEPTNTSS SEYVPEYSVE EISNEVAELD SVDPLFQCYK HVFVSLMLVR
     KMTQAAEDFL ESFGGEFDSP CCRVYRLYRH FVNEDDAPAW AIPNVVNEDS YDDYAYLREE
     LDAIDSSFEL LNEERELSEI TDRLNALRFF PVSKTEALPV ANVQEVKLIS ETYQLLMTFI
     NYSDENIPSE MPAPLLDELG MLPEELGPLN EIEDIKPVAA PITLLSEFRA SDNAKPLDIV
     EIIPDVSPTK PYEAVISGND WMTLGRIIPT TPVPTIRDVF FSGLSRHGSP EVIQNALDEF
     LPLHHSIDDK YFQEWVETSD KSLDVDPCRI DLSVFNNWQS SENCYEPRFK TGALSTRKGT
     QTEALLAIKK RNMNVPNLGQ IYDVNSVANS VVNKLLTTVI DPDKLCMFPD FISEGEVSYF
     QDYIVGKNPD PELYSDPLGV RSIDSYKHMI KSVLKPVEDN SLHLERPMPA TITYHDKDIV
     MSSSPIFLAA AARLMLILRD KITIPSGKFH QLFSIDAEAF DASFHFKEID FSKFDKSQNE
     LHHLIQERFL KYLGIPNEFL TLWFNAHRKS RISDSKNGVF FNVDFQRRTG DALTYLGNTI
     VTLACLCHVY DLMDPNVKFV VASGDDSLIG TVEELPRDQE FLFTTLFNLE AKFPHNQPFI
     CSKFLITMPT TSGGKVVLPI PNPLKLLIRL GSKKVNADIF DEWYQSWIDI IGGFNDHHVI
     RCVAAMTAHR YLRRPSLYLE AALESLGKIF AGKTLCKECL FNEKHESNVK IKPRRVKKSH
     SDARSRARRA