ID   RDRP_APRVF              Reviewed;        1233 AA.
AC   Q2Y0E9;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   23-FEB-2022, entry version 38.
DE   RecName: Full=RNA-directed RNA polymerase VP2;
DE            EC=2.7.7.48;
GN   Name=S2;
OS   Aedes pseudoscutellaris reovirus (isolate France) (ApRV).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Spinareovirinae; Dinovernavirus.
OX   NCBI_TaxID=648170;
OH   NCBI_TaxID=316597; Aedes pseudoscutellaris (Mosquito) (Stegomyia pseudoscutellaris).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=16171838; DOI=10.1016/j.virol.2005.08.028;
RA   Attoui H., Mohd Jaafar F., Belhouchet M., Biagini P., Cantaloube J.F.,
RA   de Micco P., de Lamballerie X.;
RT   "Expansion of family Reoviridae to include nine-segmented dsRNA viruses:
RT   isolation and characterization of a new virus designated Aedes
RT   pseudoscutellaris reovirus assigned to a proposed genus (Dinovernavirus).";
RL   Virology 343:212-223(2005).
CC   -!- FUNCTION: RNA-directed RNA polymerase that is involved in transcription
CC       and genome replication. Following infection, it catalyzes the synthesis
CC       of fully conservative plus strands. After core assembly, which consists
CC       in recruitment of one capped plus-strand for each genomic segments and
CC       polymerase complexes, the polymerase switches mode and catalyzes the
CC       synthesis of complementary minus-strands (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48;
CC   -!- SUBUNIT: Interacts with VP6. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the reoviridae RNA-directed RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; DQ087277; AAZ94069.1; -; Genomic_RNA.
DR   RefSeq; YP_443936.1; NC_007667.1.
DR   SMR; Q2Y0E9; -.
DR   PRIDE; Q2Y0E9; -.
DR   GeneID; 5076689; -.
DR   KEGG; vg:5076689; -.
DR   Proteomes; UP000001676; Genome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Hydrolase; Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..1233
FT                   /note="RNA-directed RNA polymerase VP2"
FT                   /id="PRO_0000403276"
FT   DOMAIN          1..775
FT                   /note="RdRp catalytic"
SQ   SEQUENCE   1233 AA;  143302 MW;  CC2B5C43577F84D2 CRC64;
     MATEPNEINL RMTQTRMKQI EDTTFKIRRK HDDMYTYLIN DIEATEMYPD TETPFRMKSS
     LNNTYIQVKC QIPKLIMQPP LYNNDMYPLN HNSTNFLEDR PFNYLCNFDW FEFLSRSKDE
     LGMHYNMIRD LISMSSQTRY SNIWSNISGI IMYLRQYKRG FALRSILIRL LTYWNNFPFF
     DTWDGFKDVL PKTSTAWPLL FYAFMSVAFD YICENISEGD AIVCFQNHLE NAQISYQDTK
     LEKKNGYTLW LKYECHNLRV ALTPRYDYTG TVYGNFLKDT TEYIITDEFE ENEIKMKNIL
     NPSFYKILQQ LRREIKTVED IIRLLTICYS ARDDRTYYGT LMELAISKAI KPQVAGSIVP
     KPIPTSWLQK DPKIVLSAKY PSTSFLSQMQ EFYRRYYPAL QREIDVHALS ASFINFLSTA
     SAGVGIELPE EIINMVKDKR LLYLLKKGSG KRVLQEALLV ERYNDLDPII NDFVRLIKIV
     VRKQIERRQR GIAGIPNNVL KINQVTYEAN KPFSKIARAP SHGKQSGNAS DIHDLLFYTT
     QEDVSHIEVN GKRQMRGIVI SSADVKGMDT HIQINAAMNQ HLGAIEILDG IQYDVGPFRQ
     TRAVIQDIQG NVYERNLNGG QQAIAFGLAN FSQTTGINSK YFGQIPNQEG TFPSGLITTS
     NHHTQMLTLL IETAATTFTN EFGKSIAIAH LMILGDDVSL MLHGNDKDVN FFMKYLVEKF
     SQLGLILERD ESRNFGVFLQ QHVINGRFNG FSNRIAIFTS EDYKTRKSVQ ESCTEYNALI
     DDVIFRTYNV RKLLQFQRIH QFVVLSKYIF RVQNYKYEKL KAKLATRLNV FEYDLKPHND
     SRENVQNQNM LRFIGIQIPY TYFQCSGGGE IPPESFQRKD GSFTYEYSIY SPKGKWLRKF
     LYDISCTIDE PKFRIDDEIM RMYNLDICDF LIQYNMLNIQ EEIRATIIDR ELISKLAMNL
     ESLENSNARM ISRRASESLR IMGIKLPANG VYGYQINERL VKVLQNIQQS DYEVKMVGDA
     LFTAIMEKFD SHKVRMEKGD RLHNFTLDFS DKSDRLVINK KMIALHNISI SKNMAPYSDA
     WMLYTCLNHT YNASSELSTA LAHSQGHFKS FQYDRDMFAE SVKIASKHGI GSLPMELFFE
     ASNIRDSAQL KWIEAIKYYI QFKDYLYPYS INPRRLFFIP EQVSSVSSLL NQDNIPADAN
     KKALMFRRAY AYVLSHPFRI SGAKAIFVDD RIS