ID   RDRP_BBMV               Reviewed;         810 AA.
AC   P27462;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Broad bean mottle virus.
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Bromovirus.
OX   NCBI_TaxID=12301;
OH   NCBI_TaxID=3906; Vicia faba (Broad bean) (Faba vulgaris).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1546462; DOI=10.1016/0042-6822(92)90470-a;
RA   Romero J., Dzianott A.M., Bujarski J.J.;
RT   "The nucleotide sequence and genome organization of the RNA2 and RNA3
RT   segments in broad bean mottle virus.";
RL   Virology 187:671-681(1992).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48;
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
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DR   EMBL; M64713; AAA42741.1; -; Genomic_RNA.
DR   PIR; A42453; P2WMBB.
DR   RefSeq; NP_658999.1; NC_004007.1.
DR   PRIDE; P27462; -.
DR   GeneID; 962139; -.
DR   KEGG; vg:962139; -.
DR   Proteomes; UP000007448; Genome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR007610; BBMV_Gp1_N.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF04522; DUF585; 1.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
PE   3: Inferred from homology;
KW   Nucleotidyltransferase; Reference proteome; RNA-directed RNA polymerase;
KW   Transferase.
FT   CHAIN           1..810
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000083268"
FT   REGION          140..160
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        146..160
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   810 AA;  92993 MW;  BDB1A5EB27D532AE CRC64;
     MSKFVAADEY IVPSFQWLLG PTSSQVESFD SSVAGWIKRY EDESKRPEST VDGSCESFVL
     AVKPVMIGGQ CEPAYDQAKW AETCLNVTNL ASGLTGVRLI PLPEMARMIY LDEEDSFVDE
     SEVDDWYPED TSDGFEYLSA DGSDYHQNSE PEEEVLDKSN GTLESEEARH ETDVKSICSF
     RDIPVETTMG HRYLALSEEF ASIEVDYQVS SIVTPLNKGS IYMTLDVHEE THPKSGIQDK
     ASIERLEAAG HNALKTHAYF DDSYYEGFEE SADFSSDFQR LKIKQSHVDW YKDPDKFFEP
     VLNFGASSRR VGSQKTVLTA LKKRNADVPE LSDVVDATEV PICLRQSYLN IYGADCLFES
     FNIMAKGLDY HKRWKSHKEL QGVTLLCETN LQRYQHMIKS DVKPTVTDTL HVERDVPATI
     TFHGKGVTSC FSPFFTACFE KFSLALRESI YVPIGKISSL ELKSKALNNK FFLEADLSKF
     DKSQGELHLE FQRLILINLG FPVPLTNWWC DFHRSILSRW TLRPGVSIPL LSKTNRGCFH
     LLWEHFSDNA MMAYCFDMST AELAMFSGDD SLVICGSKPE FDPGVFQSLF NMEVKVMDPS
     LPYICSKFLL ESEFGDVFSV PDPMREIQRL QKRKIPKDVQ VLRADFDSFC DRMKFLDRLS
     ELSLSVLCRL TALKYCKPGI EGDVRAWLSG FAYYRENFLR YSECYVTDGI HCYRRVDPMS
     RFKPIDKFQR SDKEWFHDWR NNEFPKKPID KMARMFGAYK GPVNNDRVER KAKYKVNAAM
     HDPFALAYER RYVQELELKN DKGNCSVLRE