ID   RDRP_BMV                Reviewed;         822 AA.
AC   P03594;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   23-FEB-2022, entry version 91.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Brome mosaic virus (BMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Bromovirus.
OX   NCBI_TaxID=12302;
OH   NCBI_TaxID=15371; Bromus inermis (Smooth brome grass) (Bromopsis inermis).
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=6694215; DOI=10.1016/s0022-2836(84)80012-1;
RA   Ahlquist P., Dasgupta R., Kaesberg P.;
RT   "Nucleotide sequence of the brome mosaic virus genome and its implications
RT   for viral replication.";
RL   J. Mol. Biol. 172:369-383(1984).
RN   [2]
RP   INTERACTION WITH REPLICATION PROTEIN 1A.
RX   PubMed=19325881; DOI=10.1371/journal.ppat.1000351;
RA   Liu L., Westler W.M., den Boon J.A., Wang X., Diaz A., Steinberg H.A.,
RA   Ahlquist P.;
RT   "An amphipathic alpha-helix controls multiple roles of brome mosaic virus
RT   protein 1a in RNA replication complex assembly and function.";
RL   PLoS Pathog. 5:E1000351-E1000351(2009).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a.
CC       {ECO:0000269|PubMed:19325881}.
CC   -!- INTERACTION:
CC       P03594; P03588: ORF1a; NbExp=2; IntAct=EBI-15874357, EBI-15874242;
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
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DR   EMBL; X01678; CAA25834.1; -; Unassigned_RNA.
DR   PIR; A04202; P2BVA.
DR   RefSeq; NP_041197.1; NC_002027.1.
DR   DIP; DIP-59379N; -.
DR   IntAct; P03594; 1.
DR   GeneID; 962145; -.
DR   KEGG; vg:962145; -.
DR   Proteomes; UP000001649; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IDA:UniProtKB.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039690; P:positive stranded viral RNA replication; IDA:UniProtKB.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR007610; BBMV_Gp1_N.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR018228; DNase_TatD-rel_CS.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF04522; DUF585; 1.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   1: Evidence at protein level;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..822
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000083269"
FT   DOMAIN          459..572
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   822 AA;  93927 MW;  C13B99016B2C54AC CRC64;
     MSSKTWDDDF VRQVPSFQWI IDQSLEDEVE AASLQVQEPA DGVAIDGSLA SFKLAIAPLE
     IGGVFDPPFD RVRWGSICDT VQQMVQQFTD RPLIPQAEMA RMLYLDIPGS FVLEDEIDDW
     YPEDTSDGYG VSFAADEDHA SDLKLASDSS NCEIEEVRVT GDTPKELTLG DRYMGIDEEF
     QTTNTDYDIT LQIMNPIEHR VSRVIDTHCH PDNPDISTGP IYMERVSLAR TEATSHSILP
     THAYFDDSYH QALVENGDYS MDFDRIRLKQ SDVDWYRDPD KYFQPKMNIG SAQRRVGTQK
     EVLTALKKRN ADVPEMGDAI NMKDTAKAIA KRFRSTFLNV DGEDCLRASM DVMTKCLEYH
     KKWGKHMDLQ GVNVAAETDL CRYQHMLKSD VKPVVTDTLH LERAVAATIT FHSKGVTSNF
     SPFFTACFEK LSLALKSRFI VPIGKISSLE LKNVRLNNRY FLEADLSKFD KSQGELHLEF
     QREILLALGF PAPLTNWWSD FHRDSYLSDP HAKVGMSVSF QRRTGDAFTY FGNTLVTMAM
     IAYASDLSDC DCAIFSGDDS LIISKVKPVL DTDMFTSLFN MEIKVMDPSV PYVCSKFLVE
     TEMGNLVSVP DPLREIQRLA KRKILRDEQM LRAHFVSFCD RMKFINQLDE KMITTLCHFV
     YLKYGKEKPW IFEEVRAALA AFSLYSENFL RFSDCYCTEG IRVYQMSDPV CKFKRTTEER
     KTDGDWFHNW KNPKFPGVLD KVYRTIGIYS SDCSTKELPV KRIGRLHEAL ERESLKLAND
     RRTTQRLKKK VDDYATGRGG LTSVDALLVK SHCETFKPSD LR