RDRP_BOTVF
ID RDRP_BOTVF Reviewed; 1896 AA.
AC Q9DRA1;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=RNA replication protein;
DE Includes:
DE RecName: Full=RNA-directed RNA polymerase;
DE EC=2.7.7.48;
DE Includes:
DE RecName: Full=Helicase;
DE EC=3.6.4.13;
DE Includes:
DE RecName: Full=Methyltransferase;
DE EC=2.1.1.-;
GN ORFNames=ORF1;
OS Botrytis virus F (isolate Botrytis cinerea/New Zealand/Howitt/2001)
OS (BotV-F).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Gammaflexiviridae; Mycoflexivirus.
OX NCBI_TaxID=686946;
OH NCBI_TaxID=40559; Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=11125160; DOI=10.1099/0022-1317-82-1-67;
RA Howitt R.L., Beever R.E., Pearson M.N., Forster R.L.;
RT "Genome characterization of Botrytis virus F, a flexuous rod-shaped
RT mycovirus resembling plant 'potex-like' viruses.";
RL J. Gen. Virol. 82:67-78(2001).
CC -!- FUNCTION: RNA replication protein replicates the viral genomic RNA. The
CC central part of this protein possibly functions as an ATP-binding
CC helicase and/or methyltransferase (Probable). {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
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DR EMBL; AF238884; AAG23416.1; -; Genomic_RNA.
DR RefSeq; NP_068549.1; NC_002604.1.
DR PRIDE; Q9DRA1; -.
DR GeneID; 912251; -.
DR KEGG; vg:912251; -.
DR Proteomes; UP000000398; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 4: Predicted;
KW ATP-binding; Helicase; Hydrolase; Methyltransferase;
KW Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase;
KW Reference proteome; RNA-directed RNA polymerase; Transferase;
KW Viral RNA replication.
FT CHAIN 1..1896
FT /note="RNA replication protein"
FT /id="PRO_0000402499"
FT DOMAIN 60..227
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT DOMAIN 1025..1194
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 1195..1327
FT /note="(+)RNA virus helicase C-terminal"
FT DOMAIN 1668..1774
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT REGION 828..991
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1347..1388
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 831..857
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 951..966
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1347..1385
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 996..1003
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 1059..1066
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1896 AA; 212378 MW; EE15A3ED1387B271 CRC64;
MAVPLERAFA SVTQSFHKDA TQGPTTNEYL VARAETQRYA PYAVRKGPAA LLAQVGINTI
HDSPLSHSHP ACYALNAFHF LKVIPRYLHG KAEVWGTKDN WFRKLQAQID SQTTTLTHRN
YAITARDHVR YPGTVVDHPG SCTAGTLFMH DALQYMTPLD VYTLFATSPE MHSLVATAVI
PPESVDRLPA FWPELYQLAY YEDHLCYAPD GNFADAYNQP LAAHQWMTMK SLHGPDFTLS
VDVPASRYSH HIFVISKRPG LPDTHRDFMC PDLVELPKDF FPGANKADKL LPRTLVNQMV
EYAASVKRAT IKDATSRTRA YVKDQKYAAV TPVQQMYLSW FGSGLSQMQF PDRPVTPLDN
FATALWYRLL PSRLYRLPAT IRNFQLKAFV DQLRTPQYLL HFKLDEYHLS SNDKYSDFYR
NESRQASSKD MLSAFEAICF GGAPELEKKI LGNNNPAPFA TVPNPPSQTA LGLATITAAI
AFILPNRAAR LTRLVARYAI RAVKEIVNPS FLTIPRFGLL SHSIASWLQT DGAVITYGLA
PKIASWSAPI WYNAALLAPT DMRSCQVLVD IANDLRFDYR YGTMRLAVTL GLTLLNPFLR
RLVLLPFKVL WHSYLPSIKN KLDSMVLMSI STLPDNISVP LLTLRGNLNR QRCPDNKDPT
PIHESDDNST ADDKCLHCPI HCLKSKLSEP AASPDTVPED NPLQEGDLTE EVYAAALAKK
DTPPPYPTRN DCLLVALADG LLTKRILWFS CIKMFGNTAC WVIHDTGKAF DWMHLKMLAE
QLKLNVSIEV AAEHDHHWTG LAKRVGPIDG RKIALRWYPY HWELSDRGRE DYGPPPPPPP
PSTTEPPPPP NPPAASPYHS PTVEDEPLEE IPLANSNGEF STFAFDDMAA DDLNPAQPLS
SLDLDEEPSA KQEPLEPAAI KTSEPEPLVP ESEEVFFRPS PTPTPSDATP TPAARAPSSV
SNENAQRPRH PLPPVPTGGA SAPSNDKLNS AAKQYARLVA GKTRQMTVAQ PRSKAYLRDL
KRGNIGTIPT AEAEQLDAYI DSWAVNGFNR SVHVIYFCGL PGTGKSRRCM KMTERLLKEN
KYLAQTVRIV TPTDNLRNSV ARQLKPPTES SYSVQTYETP IRQPSLGPVL IVDEYGKMPA
GWLETVLFLN PRVQMVIFTA DPTQGIFRTR IPDAYCCRIP SSTEAIASYA HEYRRVSDRP
APGVARALGL PTTSRRPGQI ETSTELNVDW ITVRPEGERA AWHNTWGGKV YTYATCQGET
FHRAYQMVIN GNTRFWDDRD LFAALTRGSG TLRLIYETQS SKPLPRSTSK LYNALLDFRT
DPHKLPLAIE EHVNRYIPSH LRNVNQAVPR RNAASNPDAL PSQRILPGGQ STTAEPXQSQ
ASALPTGGEA IDVPALDRQD PQRFPQLKVL PHLCGYIGDY SEASYPEPEH PKTQMLDATF
PTYLARNRGW NDPALTDAVT ANYKAPEHRD IWVEAVGETT RQVHGSGDDR DVFLEHRGDD
KATANITYAK RLRFSQRAAN ERSIAATKVA GSELFEAFTR AIPLPQETFN DSLLEECRTE
NDTVHLTSKP LATLINNAER SDPSWSLNMI KLFIKGQTVK KLEKMGSDAT AGQSIASFRA
EVLLAWGPYA RYIDRRIRAL LPPHVYIHSR RTNEDFEKFV AAHWDHTRES TDGDYTAYDA
SQDATFVNFE TLLMRRLDFP LDIIEAYVEM KASITSHFGP LAIMRFSGEV WTYLFNTLGN
IAFTYAKYEV PSVAQVYGGD DKSINSPITV RTGWSQLVGK FNLVEKPVVG YEPTFCGWRI
VPGGIVKDPQ LLFWRTRYAR IRYDAALWAP GYYDELVLSL KTSDRLMDHM SPNDLAYLQA
LVRFYTKLSR RLPSLADRRR CNPLPADSPS VVLSRD