ID   RDRP_BPMS2              Reviewed;         545 AA.
AC   P00585;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=RNA-directed RNA polymerase beta chain;
DE            EC=2.7.7.48 {ECO:0000269|PubMed:2202149};
DE   AltName: Full=RNA replicase beta chain;
OS   Escherichia phage MS2 (Bacteriophage MS2).
OC   Viruses; Riboviria; Orthornavirae; Lenarviricota; Leviviricetes;
OC   Levivirales; Leviviridae; Levivirus.
OX   NCBI_TaxID=329852;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1264203; DOI=10.1038/260500a0;
RA   Fiers W., Contreras R., Duerinck F., Haegeman G., Iserentant D.,
RA   Merregaert J., Min Jou W., Molemans F., Raeymaekers A., van den Berghe A.,
RA   Volckaert G., Ysebaert M.;
RT   "Complete nucleotide sequence of bacteriophage MS2 RNA: primary and
RT   secondary structure of the replicase gene.";
RL   Nature 260:500-507(1976).
RN   [2]
RP   NUCLEOTIDE SEQUENCE OF 488-545.
RX   PubMed=7411633; DOI=10.1016/0022-2836(80)90307-1;
RA   Iserentant D., van Montagu M., Fiers W.;
RT   "Studies on the bacteriophage MS2. XLI. Nature of the azure mutation.";
RL   J. Mol. Biol. 139:243-263(1980).
RN   [3]
RP   SUBUNIT.
RX   PubMed=4592692; DOI=10.1073/pnas.71.2.460;
RA   Lupker J.H., Verschoor G.J., de Rooij F.W., Roersch A., Bosch L.;
RT   "An Escherichia coli mutant with an altered elongation factor Tu.";
RL   Proc. Natl. Acad. Sci. U.S.A. 71:460-463(1974).
RN   [4]
RP   SUBUNIT.
RX   PubMed=4610580; DOI=10.1073/pnas.71.9.3611;
RA   Szer W., Leffler S.;
RT   "Interaction of Escherichia coli 30S ribosomal subunits with MS2 phage RNA
RT   in the absence of initiation factors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 71:3611-3615(1974).
RN   [5]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=2202149; DOI=10.1016/0042-6822(90)90417-p;
RA   Shaklee P.N.;
RT   "Negative-strand RNA replication by Q beta and MS2 positive-strand RNA
RT   phage replicases.";
RL   Virology 178:340-343(1990).
CC   -!- FUNCTION: This is the catalytic subunit of the viral RNA-dependent RNA
CC       polymerase complex. This complex is involved in viral RNA replication
CC       that produces (+)-stranded genomes via a complementary, (-)-stranded
CC       intermediate. {ECO:0000269|PubMed:2202149}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539,
CC         ECO:0000269|PubMed:2202149};
CC   -!- SUBUNIT: Part of the viral RNA-dependent RNA polymerase complex, the
CC       other subunits are probably the host ribosomal protein S1, EF-Tu and
CC       EF-Ts. {ECO:0000305|PubMed:4592692, ECO:0000305|PubMed:4610580}.
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DR   EMBL; V00642; CAA23991.1; -; mRNA.
DR   EMBL; M10565; AAA32263.1; -; Genomic_RNA.
DR   PIR; A00723; RRBPBM.
DR   RefSeq; NP_040650.1; NC_001417.2.
DR   SMR; P00585; -.
DR   Proteomes; UP000002127; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007096; RNA-dir_Rpol_phage_catalytic.
DR   InterPro; IPR005093; RNArep_beta.
DR   Pfam; PF03431; RNA_replicase_B; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50522; RDRP_PHAGE; 1.
PE   1: Evidence at protein level;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..545
FT                   /note="RNA-directed RNA polymerase beta chain"
FT                   /id="PRO_0000164854"
FT   DOMAIN          243..373
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   545 AA;  60837 MW;  A723402D43CD9904 CRC64;
     MSKTTKKFNS LCIDLPRDLS LEIYQSIASV ATGSGDPHSD DFTAIAYLRD ELLTKHPTLG
     SGNDEATRRT LAIAKLREAN GDRGQINREG FLHDKSLSWD PDVLQTSIRS LIGNLLSGYR
     SSLFGQCTFS NGAPMGHKLQ DAAPYKKFAE QATVTPRALR AALLVRDQCA PWIRHAVRYN
     ESYEFRLVVG NGVFTVPKNN KIDRAACKEP DMNMYLQKGV GAFIRRRLKS VGIDLNDQSI
     NQRLAQQGSV DGSLATIDLS SASDSISDRL VWSFLPPELY SYLDRIRSHY GIVDGETIRW
     ELFSTMGNGF TFELESMIFW AIVKATQIHF GNAGTIGIYG DDIICPSEIA PRVLEALAYY
     GFKPNLRKTF VSGLFRESCG AHFYRGVDVK PFYIKKPVDN LFALMLILNR LRGWGVVGGM
     SDPRLYKVWV RLSSQVPSMF FGGTDLAADY YVVSPPTAVS VYTKTPYGRL LADTRTSGFR
     LARIARERKF FSEKHDSGRY IAWFHTGGEI TDSMKSAGVR VIRTSEWLTP VPTFPQECGP
     ASSPR