ID   RDRP_BSNV               Reviewed;         867 AA.
AC   Q8AZL8;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            Short=RDRP;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein VP1;
GN   Name=VP1;
OS   Blotched snakehead virus (BSNV) (Channa lucius virus).
OC   Viruses; Riboviria; Orthornavirae; Birnaviridae; Blosnavirus.
OX   NCBI_TaxID=311176;
OH   NCBI_TaxID=64146; Channa lucius (Forest snakehead) (Ophicephalus lucius).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=12477876; DOI=10.1128/jvi.77.1.719-725.2003;
RA   Da Costa B., Soignier S., Chevalier C., Henry C., Thory C., Huet J.-C.,
RA   Delmas B.;
RT   "Blotched snakehead virus is a new aquatic birnavirus that is slightly more
RT   related to avibirnavirus than to aquabirnavirus.";
RL   J. Virol. 77:719-725(2003).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which is found both free and
CC       covalently attached to the genomic RNA. May also contain guanylyl and
CC       methyl transferase activities (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with VP3 in the cytoplasm. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=Minor amounts are
CC       incorporated in the virion. {ECO:0000250}.
CC   -!- PTM: May exist in multiple phosphorylated forms. {ECO:0000250}.
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DR   EMBL; AJ459383; CAD30691.1; -; Genomic_RNA.
DR   RefSeq; YP_052864.1; NC_005983.1.
DR   SMR; Q8AZL8; -.
DR   GeneID; 2886016; -.
DR   KEGG; vg:2886016; -.
DR   Proteomes; UP000007250; Genome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0018144; P:RNA-protein covalent cross-linking; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007100; RNA-dir_pol_birnavirus.
DR   InterPro; IPR007098; RNA-dir_pol_mononegavirus.
DR   Pfam; PF04197; Birna_RdRp; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50524; RDRP_DSRNA_BIR; 1.
PE   3: Inferred from homology;
KW   Covalent protein-RNA linkage; GTP-binding; Nucleotide-binding;
KW   Nucleotidyltransferase; Phosphoprotein; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication; Virion.
FT   CHAIN           1..867
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000227879"
FT   DOMAIN          387..591
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          845..867
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        852..867
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         249..256
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   867 AA;  96792 MW;  B1318D2AE7A75D97 CRC64;
     MSDVFNTPHA RRTITQALGL TNVSDRTQDW LLPEPWNPPM DPIKNSQEAA EYLQRNQYRM
     LKPRAIPENT PLDTSELFPH LAEFVGSGAF GTSTLVPAGS TEYIPRYYPT HKPEHNKPTP
     FGHYDVALLK QMTYQLTNGK DNPEEEGTTF RQFRDTIVEQ QYGSGTSQGQ LARLVAMKEV
     ATGRNPNKSP KELGLSMEEI ATMLEQTLPI GQPGGDEGWP SLTTTLSELL NPAEGMDYLP
     HITMKSSSGL PFIGKTKGET VTSALAICDT FLREVSECVK EGAMASDNQK LQKLLQDYWY
     LSCGLLFPKA ERYEKKAWLT KTRNIWSAPF PTHLLLSTIS WPIMNSSKNN ILNVPECVSL
     YGFNPFSGGM DAVVTNILAQ PDETLFLIYA DNIYIYMDRT WFSIDLEKGE ANATPEHAQA
     VSYYLLTRGW TQDDGSPAFN ATWATIAMMI APSLVVDSSC LFMNLQLKTY GQGSGNAWTF
     LINHTLSTIL VGKWIEAGQP NPRSKEFMDL EAATGINFKI EREIEGLPTR LQEAMDKAVH
     TGFLGDGTTN PPEKEGPTVD LDLLGWSATY SRHMDMWVPV LDKERLLSSA AYPKGLENKD
     LKGKPGAEIA YKIVRNEALL MVGGWNYPLI ARSLMANTSA ARNNLRQKGV PLDTLTRDWE
     KMTEFSDIFE DLPIDTKLEV TSEFLQRLNL RGERKQPNVN KHHLRTKGLK KCVSALKQGA
     CRNPTTVAGL KLTAYSKSRI NKAKAVFDEI NNLPKTESDD WSDRMDDADR LMKANNLYMR
     EARSALEDVH NSLLALSGET VKAKTPQEKS TEKVSNPVVG YRLPAERATG VQHALLGVGV
     SRPSEGALTK NARKMKKRRE KARGINH