ID   RDRP_BYDVR              Reviewed;         867 AA.
AC   P29045;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   29-SEP-2021, entry version 74.
DE   RecName: Full=Putative RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
GN   ORFNames=ORF1/ORF2;
OS   Barley yellow dwarf virus (isolate P-PAV) (BYDV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Luteovirus.
OX   NCBI_TaxID=31724;
OH   NCBI_TaxID=146531; Avena byzantina.
OH   NCBI_TaxID=4498; Avena sativa (Oat).
OH   NCBI_TaxID=4513; Hordeum vulgare (Barley).
OH   NCBI_TaxID=4521; Lolium multiflorum (Italian ryegrass) (Lolium perenne subsp. multiflorum).
OH   NCBI_TaxID=4522; Lolium perenne (Perennial ryegrass).
OH   NCBI_TaxID=4530; Oryza sativa (Rice).
OH   NCBI_TaxID=4550; Secale cereale (Rye).
OH   NCBI_TaxID=4565; Triticum aestivum (Wheat).
OH   NCBI_TaxID=4577; Zea mays (Maize).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1538199; DOI=10.1099/0022-1317-73-2-487;
RA   Ueng P.P., Vincent J.R., Kawata E.E., Lei C.H., Lister R.M., Larkins B.A.;
RT   "Nucleotide sequence analysis of the genomes of the MAV-PS1 and P-PAV
RT   isolates of barley yellow dwarf virus.";
RL   J. Gen. Virol. 73:487-492(1992).
CC   -!- FUNCTION: Probable polymerase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC       Name=Putative RNA-directed RNA polymerase;
CC         IsoId=P29045-1; Sequence=Displayed;
CC       Name=39 kDa protein;
CC         IsoId=P29045-2; Sequence=VSP_031896;
CC   -!- MISCELLANEOUS: [Isoform Putative RNA-directed RNA polymerase]: Produced
CC       by -1 ribosomal frameshifting between codons 339 and 340.
CC   -!- MISCELLANEOUS: [Isoform 39 kDa protein]: Produced by conventional
CC       translation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the luteoviruses RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; D11032; BAA01785.1; ALT_FRAME; Genomic_RNA.
DR   PIR; JQ1415; RRVQCM.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR013674; Luteo_Rpol_P1-P2.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   Pfam; PF08467; Luteo_P1-P2; 1.
DR   Pfam; PF00998; RdRP_3; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Ribosomal frameshifting;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..867
FT                   /note="Putative RNA-directed RNA polymerase"
FT                   /id="PRO_0000039188"
FT   DOMAIN          561..676
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   VAR_SEQ         340..867
FT                   /note="Missing (in isoform 39 kDa protein)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_031896"
FT   VARIANT         865
FT                   /note="I -> N"
SQ   SEQUENCE   867 AA;  98658 MW;  C2B724CE3DD7D001 CRC64;
     MFFEILIGAS AKAVKDFISH CYSRLKSIYY SFKRWLMEIS GQFKAHDAFV NMCFGHMADI
     EDFEAELAEE FAEREDEVEE ARSLLKLLVA QKSKTGVTEA WTDFFTKSRG GVYAPLSCEP
     TRQELEAKSE KLEKLLEEQH QFEVRAAKKY IKEKGRGFIN CWNDLRSRLR LVKDVKDEAK
     DNARAAAKIG AEMFAPVDVQ DLYSFTEVKK VETGLMKEVV KERNGEEEKH LEPIMEEVRS
     IKDTAEARDA ASTWITETVK LKNSTLNADE LSLATIARYV ENVGDKFKLD IASKTYLKQV
     ASMSVPIPTN KDIKLKMVLQ SPEARARREP LDVLDSVGFL EGLCTASGFE SPFPILGLPE
     IAVTDGARLR KVSSNIRYLS QTHLGLVYKA PNASLHNALV AVERRVFTVG KGDKAIYPPR
     PEHDIFTDTM DYFQKSIIEE VGYCRTYPAQ LLANSYSAGK RAMYHKAIAS LRTVPYHQKD
     ANVQAFLKKE KHWMTKDIAP RLICPRSKRY NIILGTRLKF NEKKIMHAID SVFGSPTVLS
     GYDNFKQGRI IAKKWQKFAC PVAIGVDASR FDQHVSEQAL KWEHGIYNGI FGDSELALAL
     EHQITNNIKM FVEDKMLRFK VRGHRMSGDI NTSMGNKLIM CGMMHAYFKK LGVEAELCNN
     GDDCVIITDR ANEKLFDGMY DHFLQYGFNM VTEKPVYELE QLEFCQSKPV SINGKYRMVR
     RPDSIGKDST TLLSMLNQSD VKSYMSAVAQ CGLVLNAGVP ILESFYKCLY RSSGYKKVSE
     EFIKNVISYG TDERLQGRRT YNETPITNHS RMSYWESFGV DPKIQQIVER YYDGLTVSAQ
     LQSVKVTTPH LQSILLSIPE NHSQIEY