ID   RDRP_CARMS              Reviewed;         763 AA.
AC   Q9J5U7; Q9J5U6; Q9Q6X8;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   29-SEP-2021, entry version 68.
DE   RecName: Full=Probable RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein p88;
DE   Contains:
DE     RecName: Full=Protein p28;
GN   ORFNames=ORF1;
OS   Carnation mottle virus (isolate China/Shanghai) (CarMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Alphacarmovirus.
OX   NCBI_TaxID=652111;
OH   NCBI_TaxID=278075; Dianthus barbatus.
OH   NCBI_TaxID=3570; Dianthus caryophyllus (Carnation) (Clove pink).
OH   NCBI_TaxID=118431; Dianthus chinensis.
OH   NCBI_TaxID=288950; Dianthus superbus.
OH   NCBI_TaxID=3572; Saponaria officinalis (Common soapwort) (Lychnis saponaria).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Zhang A.P., Yue Y., Ye R., Zhu H.Q., Xu L., Yu S.Q.;
RT   "Infectivity of full-length cDNA of carnation mottle virus.";
RL   Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-dependent RNA polymerase that plays an essential role in
CC       the virus replication. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- MISCELLANEOUS: Readthrough of the terminator UAG occurs at position
CC       246.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF15527.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AF192772; AAF15524.1; -; Genomic_RNA.
DR   EMBL; AF192772; AAF15525.1; -; Genomic_RNA.
DR   EMBL; AF192772; AAF15527.1; ALT_SEQ; Genomic_RNA.
DR   Proteomes; UP000006709; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   Pfam; PF00998; RdRP_3; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   4: Predicted;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA suppression of termination; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..763
FT                   /note="Probable RNA-directed RNA polymerase"
FT                   /id="PRO_0000398302"
FT   CHAIN           1..245
FT                   /note="Protein p28"
FT                   /id="PRO_0000398303"
FT   DOMAIN          467..580
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   763 AA;  85965 MW;  ECDBAC527714F3D1 CRC64;
     MGLPSLLVEG VIGCTLVGGL VAVGSAALAV RATIGVVEFN RECVRGARRI VSSGGRCLIV
     QSPYGNPNQG LIRGEDEEID NVEESTPEEL TQLIEVKAEV DGKEVVVSKK RVVNRHLRQR
     FVRSIAIEAK NHFGGDISPS KANYLSVSKF LTGKCKERHV VPAHTRDCVS AAMVLVFTPD
     VHEIRMMAGL ASDAAYARKI AMASILNRKG WCWRLMVNPL DRARWWEMWC VVNGFDSNKP
     VTFPKXGGLF YLNGVETKIR RGGHPSVIEV DGQCPPRERK LYVQNAITTG YEYRVHNHSY
     ANLRRGLLER VFYVERNKEL VSCPQPEPGS FKEMGYLRRR FHRVCGNHTR ISANDLVDCY
     QGRKRTIYEN AAASLLDRAI ERKDGDLKTF IKAEKFNVNL KSDPAPRVIQ PRSPRYNVEL
     GRYLKKYEHH AYKALDKIWG GPTVMKGYTT EEVAQHIWSA WNQFQTPVAI GFDMSRFDQH
     VSVAALEFEH SCYLACFEGD AHLANLLKMQ LVNHGVGFAS NGMLRYTKEG CRMIGDMNTA
     LGNCLLACLI TKHLMKIRSR LINNGDDCVL ICERTDIDYV VSNLTTGWSR FGFNCIAEEP
     VYEMEKIRFC QMAPVFDGAG WLMVRDPLVS MSKDSHSLVH WNNETNAKQW LKSVGMCGLR
     IAGGVPIVQE FYQKYVETAG NVKENKNITE KSSSGFFMMA DRAKRGYSAV SEMCRFSFYQ
     AFGITPDQQI ALEGEIRSLT IDTNVGPQCE AADSLWILNR KYQ