ID   RDRP_CCMV               Reviewed;         808 AA.
AC   P20179;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Cowpea chlorotic mottle virus (CCMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Bromovirus.
OX   NCBI_TaxID=12303;
OH   NCBI_TaxID=3847; Glycine max (Soybean) (Glycine hispida).
OH   NCBI_TaxID=3917; Vigna unguiculata (Cowpea).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2773323; DOI=10.1016/0042-6822(89)90134-7;
RA   Allison R.F., Janda M., Ahlquist P.;
RT   "Sequence of cowpea chlorotic mottle virus RNAs 2 and 3 and evidence of a
RT   recombination event during bromovirus evolution.";
RL   Virology 172:321-330(1989).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
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DR   EMBL; M28817; AAA46371.1; -; Genomic_RNA.
DR   PIR; JU0117; P2WMCC.
DR   RefSeq; NP_613275.1; NC_003541.1.
DR   GeneID; 962150; -.
DR   KEGG; vg:962150; -.
DR   Proteomes; UP000008445; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR007610; BBMV_Gp1_N.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF04522; DUF585; 1.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..808
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000083270"
FT   DOMAIN          470..583
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   808 AA;  92937 MW;  D39AFFE56D29B2BE CRC64;
     MSKFIPEGET YHVPSFQWMF DQTLESDSHH DEAIFVTESI NESGVDTSVE ITADGTLASY
     MHAVKPLVED GLLNPPFDQA RWGLCCKNVV DVYDGLLGYR LIPMAEAARM LYLEIDGSFV
     DESECDDWRP VDTSDGFTEA MFDVMNEIPG EETKNTCALS LEAESRQAPE TSDMVPSEYT
     LADRYVTTRE EFASVDSDYD ISLNLVSPVE FRVGVCEDTY RHSEADDPTM PQYHDRISLK
     SLEAAGHHML PTHAYFDDTY YQALEELGDY NVDISKLSVR QSDVDWYRDP EKYYEPELSI
     GSFQRRIGTQ KTVLTALKKR NADVPELADS VDIKRVACEV AEKFKRAYLN HSGIGLLGQS
     MDVMSRGLEY HKKWKDHKDL TGVTVLSEIN LQRYQHMIKS DIKPVVSDTL HLERAVAATI
     TFHGKGVTSC FSPYFTACFE KFSKALKSRF VVPIGKISSL ELKNVPLSNK WFLEADLSKF
     DKSQGELHLE FQREILLSLG FPAPLTNWWC DFHRESMLSD PHAGVNMPVS FQRRTGDAFT
     YFGNTLVTMA MMAYCCDMNT VDCAIFSGDD SLLICKSKPH LDANVFQSLF NMEIKVMDPS
     LPYVCSKFLL ETEMNNLVSV PDPMREIQRL AKRKIIKSPE LLRAHFESFC DRMKFLNKLD
     EKMINLLCKF VALKYKKPDV ENDVRVAIAA FGYYSENFLR FCECYATEGV NIYKVKHPIT
     QEWFEASRDR DGDWFHDWRN PKFPTALDKV WRFFGKYARD DPMKHIEERD RRHRLNRAMN
     SSLKLAYDRR SLSKDKETVA WVRKTLSK