ID   RDRP_CMVII              Reviewed;         857 AA.
AC   Q83262;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   29-SEP-2021, entry version 65.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Cucumber mosaic virus (strain Iizuka) (CMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Cucumovirus.
OX   NCBI_TaxID=117113;
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=3562; Spinacia oleracea (Spinach).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Karasawa A., Ito A., Okada I., Hase S., Ehara Y.;
RT   "A possible role of RNA 2 of cucumber mosaic cucumovirus as a determinant
RT   of infection phenotype on cowpea.";
RL   Nihon Shokubutsu Byori Gakkaiho 63:289-297(1997).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
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DR   EMBL; D16406; BAA03890.1; -; Genomic_RNA.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA-directed RNA polymerase;
KW   Transferase; Viral RNA replication.
FT   CHAIN           1..857
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000083274"
FT   DOMAIN          511..624
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          785..857
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        801..840
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   857 AA;  96710 MW;  A9D8FCC15E0FAD39 CRC64;
     MAFPAPAFSL ANLLNGSYGV DTPEDVERLR SEQREEAAAA CRNYRPLPAV DVSESVSEDA
     HSLQTPDGAP AEAVSDEFVT YGAEDYLEKS DDELLVAFET MVKPMRIGQL WCPAFNKCSF
     ISSIAMARAL LLAPRTSHRT MKCFEDLVAA IYTKSDFYYS EECEADDIQM DISSRDVPGY
     SFEPWSRTSG FEPPPICEAC DMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVDNA
     TLLSNLGPFL VPVKCQYEKC PTPTIAIPPN LNRATDRVDI NLVQSICDST LPTHSNYDDS
     FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK
     RNADVPELGD SVNLSRLSKA VAERFFISYI NGNSLASSNF VNVVSNFHDY MEKWKSSGLS
     YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKSITS QFSPLFTALF
     ERFQRCLRER IILPVGKISS LEMAGFDVKS KYCLEIDLSK FDKSQGEFHL LIQEHILNGL
     GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD
     QFEKLLFSGD DSLGFSLLPP VGDPSKFTTL YNMEAKVMEP SVPYICSKFL LSDEFGNTFS
     VPDPLREVQR LGTKKIPYSD NDEFLFAHFM SFVDRLKFLD RMSQSCIDQL SIFFELKYKK
     SGEEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFC STEFRVERVN SNKLRKKYGI
     ERRCDDKRRT PTGSYGGGEE AETKVSQTKS TGTRSQKSQR ESAFKSQTVP LPTVLSSGWS
     GTDRVVPPRE RGGVTRA