ID   RDRP_CMVIX              Reviewed;         858 AA.
AC   Q66117;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   29-SEP-2021, entry version 67.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Cucumber mosaic virus (strain Ixora) (CMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Cucumovirus.
OX   NCBI_TaxID=117114;
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=3562; Spinacia oleracea (Spinach).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=7561763; DOI=10.1099/0022-1317-76-9-2257;
RA   McGarvey P.B., Tousignant M., Geletka L., Cellini F., Kaper J.M.;
RT   "The complete sequence of a cucumber mosaic virus from Ixora that is
RT   deficient in the replication of satellite RNAs.";
RL   J. Gen. Virol. 76:2257-2270(1995).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
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DR   EMBL; U20218; AAC54616.1; -; Genomic_RNA.
DR   PIR; A71392; A71392.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA-directed RNA polymerase;
KW   Transferase; Viral RNA replication.
FT   CHAIN           1..858
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000083275"
FT   DOMAIN          511..624
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          775..830
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        775..807
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        808..830
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   858 AA;  97045 MW;  AE0151CA566B4005 CRC64;
     MAFPAPAFSL ANLLNGTYGV DTPEEVERVR SEQREEAAAA CRNYKPLPAV DVGASVTEDA
     HSLRTPDGAP SEEVSVEFVT YGAEDYLEKS DDELFVAFET MVKPMCIGQL WCPAFNKCSF
     ISSIAMARAL LLVPNTPNRT MKCFEDLVAA IYTKSDFYYD DECEADDVQI NISSRDVPGY
     SFEPWSRTSG FEPPPICEAC NMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVDNA
     TLLSNLGPFL VPVKCQYKQY PTPTVAIPPS LNRATDRVDI NLVQSICDST LPTHSNYDDS
     FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK
     RNADVPELGD SVNLPRLSKA VAERFFIAYI NGNSLATSNF VNVVSNFHDY MEKWKSSGLS
     YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKGITS QFSPLFTALF
     ERFQRCLRER VILPVGKISS LEMSGFDVKN KYCLEIDLSK FDKSQGEFHL MIQEHILNGL
     GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAF TYFGNTIVTM AEFAWCYDTD
     QFEKLLFPGD DSLGFSVLPP VGDPSKFTTL FNMEAKVMEP AVPYICSKFL LSDEFGNTFS
     VPDPLREVQR LGTKKIPYSD NDEFLFAHFM SFVDRLKFLD RMTQSCIDQL SLFFELKYRK
     SGAEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFS CVELRIERSS FIKQRKKKDG
     IERRRNDKRR TPTSPHGGGE ETETKVSQEE STGTMLQKSQ RESAFKSQTI PFPTVLSSRR
     FGIDRDVPPR ECGGIVRV