ID   RDRP_CMVQ               Reviewed;         839 AA.
AC   P06012;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   29-SEP-2021, entry version 80.
DE   RecName: Full=RNA-directed RNA polymerase 2a;
DE            Short=protein 2a;
DE            EC=2.7.7.48;
GN   ORFNames=ORF2a;
OS   Cucumber mosaic virus (strain Q) (CMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Martellivirales; Bromoviridae; Cucumovirus.
OX   NCBI_TaxID=12310;
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
OH   NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=6547906; DOI=10.1111/j.1432-1033.1984.tb08370.x;
RA   Rezaian M.A., Williams R.H.V., Gordon K.H.J., Gould A.R., Symons R.H.;
RT   "Nucleotide sequence of cucumber mosaic virus RNA 2 reveals a translation
RT   product significantly homologous to corresponding proteins of other
RT   viruses.";
RL   Eur. J. Biochem. 143:277-284(1984).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 3 RNA segments, RNA1, RNA2 and RNA3. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-directed
CC       RNA polymerase family. {ECO:0000305}.
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DR   EMBL; X00985; CAA25494.1; -; Genomic_RNA.
DR   PIR; S07226; S07226.
DR   Proteomes; UP000008454; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..839
FT                   /note="RNA-directed RNA polymerase 2a"
FT                   /id="PRO_0000083280"
FT   DOMAIN          508..621
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          773..824
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        791..821
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   839 AA;  94440 MW;  42C8E0B9D56A8BD9 CRC64;
     MISPPPTFSF ANLLNGSYGV DTPEEVERVR REQREDAEAA LRNYKPLPAV DVSESVPRDE
     PIVSQTVTAA PVTSVDDAFV SFGAEDYLEM SPSELLSAFE LMVKPLRVGE VLCSSFDRSL
     FISSVAMART LLLAPLTSTR TLKRFEDLVA AIYLKTDFFL EDDGPQTDVS QSDVPGYIFE
     PGQHSSGFEP PPICAKCDLI LYQCPCFDFN ALRESCAEKT FSHDYVIEGL DGVIDNATLL
     SNLGPFLLPV HCSYSKTEDP DFVVDPSLAR PTDRVDVHVV QAVCDTTLPT HGNYDDSFHQ
     VFVDSADYST DMDHVRLRQS DLVAKIPDGG HMLPVLNTGS GHQRVGTTKE VLTAIKKRNA
     DVPELGDSVN LSRLSKAVAE RFRLSYMNVD ALAKSNFVNV VSNFHAYMQK WPSSGLSYDD
     LPDLHAENLQ FYDHMIKSDV KPVVTDTLNV DRPVPATITF HKKTITSQFS PLFISLFERF
     QRCLRERVVL PVGKISSLEM TGFSVLNKHC LEIDLSKFDK SQGEFHLMIQ EHILNDLGCP
     APITKWWCDF HRFSYIKDKR AGVGMPISFQ RRTGDAFTYF GNTIVTMAEF AWCYDTDQFD
     RLLFSGDDSL AFSKLPPVGD PSKFTTLFNM EAKVMEPAVP YICSKFYSLM SLVTRFQSPT
     IREIQRLGTK KIPYSDNNDF LFAHFMSFVD RLKFMDRMSQ SCIDQLSIFF ELKYKKSGNE
     AALVLGAFKK YTANFNAYKE LYYSDRQQCD LVNTFCISEF RVIRRTTVKK KKNGCVDSSG
     VDRRPPLSQF AGGETSKTKV SRQKPASEGL QKSQRESAIY SETFPDVTIP RSRSRGLVS