ID   RDRP_CNV                Reviewed;         818 AA.
AC   P15187;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein p92;
DE   Contains:
DE     RecName: Full=Protein p33;
GN   ORFNames=ORF1;
OS   Cucumber necrosis virus (CNV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Tombusvirus.
OX   NCBI_TaxID=12143;
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2705296; DOI=10.1016/0042-6822(89)90150-5;
RA   Rochon D.M., Tremaine J.H.;
RT   "Complete nucleotide sequence of the cucumber necrosis virus genome.";
RL   Virology 169:251-259(1989).
CC   -!- FUNCTION: RNA-dependent RNA polymerase that plays an essential role in
CC       the virus replication. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- MISCELLANEOUS: Readthrough of the terminator UAG occurs at position
CC       297.
CC   -!- SIMILARITY: Belongs to the tombusviridae RNA polymerase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA42902.1; Type=Miscellaneous discrepancy; Note=Amino acid integrated instead of terminator codon missing.; Evidence={ECO:0000305};
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DR   EMBL; M25270; AAA42902.1; ALT_SEQ; Genomic_RNA.
DR   PIR; JA0130; RRVGCN.
DR   RefSeq; NP_040953.2; NC_001469.1.
DR   PRIDE; P15187; -.
DR   GeneID; 1493946; -.
DR   KEGG; vg:1493946; -.
DR   Proteomes; UP000008565; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   InterPro; IPR013707; Tombusvirus_p33.
DR   Pfam; PF00998; RdRP_3; 1.
DR   Pfam; PF08500; Tombus_P33; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA suppression of termination;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..818
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000041332"
FT   CHAIN           1..296
FT                   /note="Protein p33"
FT                   /id="PRO_0000041333"
FT   DOMAIN          524..641
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   818 AA;  92120 MW;  0CE624521246706A CRC64;
     MDTIKRMLWP KKEIFVGTFA TGVERDTSVD IFQLVCRVVL RYMRTGKIEN NTDSLGNFIV
     ELLKTDCAAK WEWFMKRRRV GDYAKSLAIA SIPVIPLLSY ATMKKTVALR AFGNELSFNI
     RVPRPSVPKK GLLLRLAAGL ALAPICALAM YATLPREKLS VFKLRTEARA HMEDEREATD
     CLVVEPAREL KGKDGEDLLT GSRMTKVIAS TGRPRRRPYA AKIAQVARAK VGYLKNTPEN
     RLIYQRVMIE IMDKDCVRYV DRDVILPLAI GCCFVYPDGV EESAALWGSD ESLGVKXGGL
     VRLPGVVTQT NRDIPSGVLL PQEVLEVRAG PPNAKDRNIF MVAGCPSQAR FLVHNHCLKN
     LKRGLVERVF CVERNGQLTR TPQPTKGAFG RLSPFRKAVC EKVGVAHRLG YDGFLSYYSG
     AKLRTYTRAV ESLHITPVSE RDSHLTTFVK AEKISTAKGD PAPRVIQPRN PRYNVELGRY
     LRHMESKLMK AVDGVFGETT CIKGYTADEV GQIFRDKWDR FNKPVAIGLD ASRFDQHCSV
     EALQFEHGFY RAMYPGNKLL SKLLDWQLHN KGKGYVPDGT ITYRKEGCRM SGDINTSLGN
     YLLMCAMIYG YMRHLGINEY SLANCGDDCV LIVERRNLKQ IQGTLPEYFL NLGYTMKVEP
     PVFQLEEVEF CQAHPVQFQG GWKMVRNVRT AMSKDVHCVN NIRDLATRRA WSNAQHHGGV
     ALTSGIPVVE KFYSRFTLYE VPKKHQRIDT VTNVHKWRGS GGDYVVTPEA RASFWAAFGL
     TGDEQLALED RLDRWEMDLF GEEGVDAHEP SILDSAVA