ID   RDRP_CPMV1              Reviewed;         809 AA.
AC   Q33339;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   29-SEP-2021, entry version 67.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=p93;
OS   Cryphonectria parasitica mitovirus 1 (strain American chestnut tree/New
OS   Jersey/NB631) (CMV1) (Cryphonectria mitovirus 1).
OC   Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes;
OC   Cryppavirales; Mitoviridae; Mitovirus.
OX   NCBI_TaxID=186769;
OH   NCBI_TaxID=5116; Cryphonectria parasitica (Chestnut blight fungus) (Endothia parasitica).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=7521532; DOI=10.1073/pnas.91.18.8680;
RA   Polashock J.J., Hillman B.I.;
RT   "A small mitochondrial double-stranded (ds) RNA element associated with a
RT   hypovirulent strain of the chestnut blight fungus and ancestrally related
RT   to yeast cytoplasmic T and W dsRNAs.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:8680-8684(1994).
CC   -!- FUNCTION: RNA-directed RNA polymerase that replicates the viral (+) and
CC       (-) genome. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48;
CC   -!- SUBUNIT: Forms a ribonucleoprotein complex with the viral RNA. Since
CC       the viral RNA is not encapsidated, ribonucleoprotein complex formation
CC       appears to be the strategy to survive in the host as persistent virus.
CC   -!- SUBCELLULAR LOCATION: Host mitochondrion. Note=The virus probably
CC       exists as a ribonucleoprotein viral particle and is transmitted via
CC       maternal inheritance through ascospores with a high rate of
CC       transmission through conidia.
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DR   EMBL; L31849; AAA61703.1; -; Genomic_RNA.
DR   RefSeq; NP_660174.1; NC_004046.1.
DR   GeneID; 949211; -.
DR   KEGG; vg:949211; -.
DR   Proteomes; UP000001105; Genome.
DR   GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR008686; RNA_pol_mitovir.
DR   PANTHER; PTHR34456; PTHR34456; 1.
DR   Pfam; PF05919; Mitovir_RNA_pol; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
PE   3: Inferred from homology;
KW   Host mitochondrion; Nucleotidyltransferase; Reference proteome;
KW   Ribonucleoprotein; RNA-binding; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..809
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000409223"
SQ   SEQUENCE   809 AA;  93178 MW;  100D93C327B98E57 CRC64;
     MAMIIHDPVY KLWYAXRAKS NLPGLAPHKT ESITTVLSLT NVKNKQATNS ILLNKITDLV
     LGLKAFSIKS KGVRTTLRVN KHWITPKEFP RFVKLVVWCT RTQEHEDSFM KIIGKCDHIW
     QTAGPNFLFK YLKEVMRLSV RRIANIELEP SKKIFVKLNK FRFPNIIPLP ICDQIIRDQN
     DQVLWASKRL IICLLTILSV HRVLPTKVVP DYSTIVDPFT GVSKTIDQKL LRKAIHLLNI
     KRVKQLKLKI TGSMKAGPNG KISLLTSSVD ALSFITQPTK IFTYLDFSVR VYKFRGLLLW
     MWMMCILLIT LPYAIVSFML GALIPIMGKL SVVYDQAGKA RIVAITNSWI QTAFYSLHLH
     VFKLLKNIDQ DGTFDQERPF KLLIKWLNEP TQKFYGFDLT AATDRLPIDL QVDILNIIFK
     NSPGSSWRSL LRIKYKSPQG FLTYAVGQPM GAYSSFAMLA LTHHVIVQVA ALNSGFTTRF
     TDYCILGDDI VIAHDTVASE YLKLMETLGL SISSGKSVIS SEFTEFAKKL KGRNNFDIFY
     RSWFSIIHFE KQILHLCTVF ELLRRGVCEL YDLYPQYINK LPKIYLRYNL LIDWVVVAFT
     NQILIGDRPR ADGIRLFDYF VGLEVIPPLL RIMLHTIKKD WNGLWNSIKY TLNKGFVVSQ
     VRVGLPDWTE LFLLPILPST YIIIRDYCRS FNDLTKLFGE WWLLRFESES YQVSILDVID
     RLAHTSIPNL DIHDKKKVKL TLDNLYKLSL IVNIPSGGAR RYIEFLRFNG LKSPLIVERY
     IKDGIRIEKP LTLQGLHRSG DIQLGFKIS