ID   RDRP_CRSVL              Reviewed;         769 AA.
AC   Q66096; Q66097;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   29-SEP-2021, entry version 54.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein p88;
GN   ORFNames=ORF1;
OS   Carnation ringspot virus (isolate Lommel) (CRSV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Regressovirinae; Dianthovirus.
OX   NCBI_TaxID=652597;
OH   NCBI_TaxID=278075; Dianthus barbatus.
OH   NCBI_TaxID=3570; Dianthus caryophyllus (Carnation) (Clove pink).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8113736; DOI=10.1099/0022-1317-75-1-243;
RA   Ryabov E.V., Generozov E.V., Kendall T.L., Lommel S.A., Zavriev S.K.;
RT   "Nucleotide sequence of carnation ringspot dianthovirus RNA-1.";
RL   J. Gen. Virol. 75:243-247(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Sit T.L., Lommel S.A.;
RL   Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probable polymerase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC       Name=88 kDa protein;
CC         IsoId=Q66096-1; Sequence=Displayed;
CC       Name=protein p27;
CC         IsoId=Q66096-2; Sequence=VSP_039744;
CC   -!- MISCELLANEOUS: [Isoform 88 kDa protein]: Produced by ribosomal
CC       frameshifting between codons 236 and 237.
CC   -!- MISCELLANEOUS: [Isoform protein p27]: Produced by conventional
CC       translation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the tombusviridae RNA polymerase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA20212.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; L18870; AAA20211.1; -; Genomic_RNA.
DR   EMBL; L18870; AAA20212.1; ALT_SEQ; Genomic_RNA.
DR   RefSeq; NP_619711.1; NC_003530.1.
DR   PRIDE; Q66096; -.
DR   GeneID; 956637; -.
DR   KEGG; vg:956637; -.
DR   Proteomes; UP000000574; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   Pfam; PF00998; RdRP_3; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   Ribosomal frameshifting; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..769
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000398304"
FT   DOMAIN          463..579
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   VAR_SEQ         237..767
FT                   /note="Missing (in isoform protein p27)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_039744"
SQ   SEQUENCE   769 AA;  87776 MW;  0B9FBFD6D28949AA CRC64;
     MAIFELFSFD IDKLLVWVSK FSPGKILSAI CRLGLDSWNN FRKWFWGLNF DKHEWAVDHF
     MPLMPRFSSD MDEVAKRIVT PKSKPKLEDC LEIDTAVEEC FNEECFEPQE DGSMKLKRVA
     APQQIKRVRT GMIEDAISAV EARIRNRHMI MGDDMGLVDE AAVRATAMDI CGEYKINEHH
     TRCIIYAAAY RAMTPDQESI DATKMAYNPK SQARRDLVSI LRKNISFGGF KSLEDFLSAP
     VSFPVEDAPY QILGIPEIKV ADKRASRVCK FKRVVGLPSL SAGQSVCVHK TSLHNMIVSL
     EQRVFRVKNE TGEFVVPPQP SKGAFDSISY FREAWKKKLY SKGPVVKSSI DDVVACYSSE
     KKKLYQKGAA TLSHRPLHWR DSKVRAFIKV EKLECDKKAP VPRTIQPRSK RYNLCIGRYL
     RLNEKRMLDA IDAVFGEKTV LSGLDNKAQG RAIAKKWSKY ESPIGIGLDA SRFDQHCSKD
     ALKFEHSFYR ECFPDDKTSP DLLDWQLENE GSALMPTESL VKYRTRCRMS GDINTGLGNK
     ILMCSMVHAY LKEVGVNASL ANNGDDCVLF CEKGDFNRIN DSLREWFLCR GFNMVVEEPV
     ECLERVVFCR SQPVCVATKW AMVRQLGSLS RDCFSTQNWL NPTTFRDAMN ALGQCNGIIN
     DGVPVHMAQA KRMYAAGGNR KFDLKALHKQ MEYSWRDRLG ARTNLLWSEV EDSTRLSYFR
     AFGIEPAVQR IVEGYFSESK ISEEGRQTNF LPTHYSRLHK DLLVPRYLN