ID   RDRP_CRV                Reviewed;         818 AA.
AC   P17459; Q9WJD6;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   29-SEP-2021, entry version 94.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
DE   AltName: Full=Protein p92;
DE   Contains:
DE     RecName: Full=Protein p33;
GN   ORFNames=ORF1;
OS   Cymbidium ringspot virus (CymRSV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Tombusvirus.
OX   NCBI_TaxID=12144;
OH   NCBI_TaxID=14366; Cymbidium.
OH   NCBI_TaxID=3899; Trifolium repens (Creeping white clover).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2771646; DOI=10.1093/nar/17.15.6383;
RA   Grieco F., Burgyan J., Russo M.;
RT   "The nucleotide sequence of cymbidium ringspot virus RNA.";
RL   Nucleic Acids Res. 17:6383-6383(1989).
CC   -!- FUNCTION: RNA-dependent RNA polymerase that plays an essential role in
CC       the virus replication. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- MISCELLANEOUS: Readthrough of the terminator UAG occurs at position
CC       297.
CC   -!- SIMILARITY: Belongs to the tombusviridae RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; X15511; CAA33532.1; -; Genomic_RNA.
DR   EMBL; X15511; CAB38439.1; -; Genomic_RNA.
DR   PIR; S05456; RRVGCR.
DR   RefSeq; NP_613260.1; NC_003532.1.
DR   RefSeq; NP_613261.1; NC_003532.1.
DR   GeneID; 935900; -.
DR   GeneID; 935901; -.
DR   KEGG; vg:935900; -.
DR   KEGG; vg:935901; -.
DR   Proteomes; UP000008567; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   InterPro; IPR013707; Tombusvirus_p33.
DR   Pfam; PF00998; RdRP_3; 1.
DR   Pfam; PF08500; Tombus_P33; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA suppression of termination;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..818
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000041334"
FT   CHAIN           1..296
FT                   /note="Protein p33"
FT                   /id="PRO_0000041335"
FT   DOMAIN          524..641
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   818 AA;  91948 MW;  DC4E84499410F42D CRC64;
     METIKRLIWP KKEIFVGDFA IGVNRTVPVD VFQLVCRVVL RYMRTGKIEC ESDSLSKFVV
     ELLKTDCAAK WEWFMKRRQG GDYIVPLSIA TIPLMPLLSC TTSVRAVSVG VLGCGFSSNI
     PIPRLSVPRK GLLLRLAAGL ALAPICALAV YATLPREKLS VYKLRTEART HMEDEKEATD
     CLVVESAREL KGKDGEDLLT GSRMTKVVAS TGRRRRTPYA AKVAQVARAK VGYLRNTPEN
     RLIYQRVIIE IMDKDCVRYV DRDVILPMAI GCCFVYQDGV EESAALWGSQ DSLGVKXGGL
     VRLPGVVTQI NRDIPSDVLL PQEVLEVRTG PPIAKDRNIF MVAGCPSQAR FLVHNHCLKN
     LKRGLVERVF CVEKDGKLTR TPQPTKGSFG RLSPFRKAVC EKVGVAHRLG YDGFLSYYSG
     AKLRTYTRAV ESLHITPVSE RDSHLTTFVK AEKISTSKGD PAPRVIQPRN PRYNVELGRY
     LRHMESKLMK AVDDVFGETT CIKGYTADEV GAIFRRKWDR FDKPVAIGLD ASRFDQHCSV
     EALQYEHSFY RALYPGNKLL SKLLEWQLHN KGKGYVPDGT ITYKKEGCRM SGDINTSLGN
     YLLMCAMVHG YMRHLGINEF SLANCGDDCV LIIERRNLKR VQGTLPGYFL NLGYTMKVES
     PVFQLEEVEF CQAHPVQFQG GWKMVRNVRT AMSKDVHCVN NIRDLATRRA WSNAQHHGGL
     ALSAGIPVVE RFYSRFPLYD MPVKHQRIDT VTNVHKWRGS GGNYHVTPES RASFWAAFGL
     TGDEQVALED RLDRWEMDLF GREGVDAHEP SILDSAVA