ID   RDRP_FXMV               Reviewed;        1335 AA.
AC   P22168;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=RNA replication protein;
DE   AltName: Full=152 kDa protein;
DE   AltName: Full=ORF1 protein;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=Helicase;
DE              EC=3.6.4.13;
OS   Foxtail mosaic virus.
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Alphaflexiviridae; Potexvirus.
OX   NCBI_TaxID=12179;
OH   NCBI_TaxID=4555; Setaria italica (Foxtail millet) (Panicum italicum).
OH   NCBI_TaxID=4556; Setaria viridis (Green bristlegrass) (Setaria italica subsp. viridis).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1840610; DOI=10.1099/0022-1317-72-9-2173;
RA   Bancroft J.B., Rouleau M., Johnston R., Prins L., Mackie G.A.;
RT   "The entire nucleotide sequence of foxtail mosaic virus RNA.";
RL   J. Gen. Virol. 72:2173-2181(1991).
CC   -!- FUNCTION: RNA replication. The central part of this protein possibly
CC       functions as an ATP-binding helicase (Probable). {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the potexviruses/carlaviruses RNA replication
CC       protein family. {ECO:0000305}.
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DR   EMBL; M62730; AAA43826.1; -; Genomic_RNA.
DR   PIR; JQ1258; JQ1258.
DR   RefSeq; NP_040988.1; NC_001483.1.
DR   SMR; P22168; -.
DR   PRIDE; P22168; -.
DR   GeneID; 1494009; -.
DR   KEGG; vg:1494009; -.
DR   Proteomes; UP000008623; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR002588; Alphavirus-like_MT_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF00978; RdRP_2; 1.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   Pfam; PF01660; Vmethyltransf; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..1335
FT                   /note="RNA replication protein"
FT                   /id="PRO_0000222548"
FT   DOMAIN          58..225
FT                   /note="Alphavirus-like MT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT   DOMAIN          564..729
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          730..863
FT                   /note="(+)RNA virus helicase C-terminal"
FT   DOMAIN          1102..1209
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          428..492
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        465..482
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         603..610
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1335 AA;  152318 MW;  D2ECBBA932F49DB9 CRC64;
     MSIEAVFDQV TDPSLRAVIQ EEAHKQIKDL FKETTRCNPY SIPQAGRKVL EKYAIPYNPY
     SLKLHPHAAS KAFEVSLYEA ASNYLPSTSS TPVTFMFTKP GKLRFFRRRG HVDKFVNADI
     VPRDLARYPR DTVYSYLPEI TTTHAFIGDT LHHFGEDFLV EVFSRSPKLE VLLATMVLPP
     EAFYRMESLH PSVYTLLYRD DRFLYLPGGL SGGEYEHRYK DLNWLTFGTV THGGITITGE
     RIETKAANHL FLFRRGRLAT PKFRSFDMPE PMVLLPKVFR PAKYNVQKPI PREKANKWLM
     YVKSIGNATI RDVWAKLRQT IANADIGLFS PTELVHLTNY FLLLGRLDSH NSFDQVLADS
     VLKAWFRPMV AKLQEIKHKL MGQTQFMQLC QALEMTEVDL VFEVRDSKTP HKQAVPLDRE
     IENVLLEGVS SEPTYTETEG VADGPLPPPM QTAAEPSATS DEPESSSSRE IEHQPAPEIT
     LDEEEPQRDD LPWDAWRTQL RALGFEASER QYDPDGELIS PILSTRRLPK TPIDTTLYAT
     LDKIARCPTF YKPDTDRAQT YARDVMAGKT GAILKQQPFE WKTTLKRKTK EEPKEIHLAV
     LHGAGGSGKS YALQEFMRNN SDTPITVILP TNELRADWKK KLPAHDKDTF MTYENALLCP
     RGDIFIMDDY TKLPRGYIEA FVQNAPALSL LILTGDPNQA EHFETTEDNE INSLAPASVV
     FGKFSRYHIN ATHRNPRNLA NALGVYSETP GEVKVLYTRN IKTGYHNLVP SQMKMRNYAS
     LGQRASTYAG CQGITAPRVQ IILDSDTPRC TRQVMYTALS RATTEVVLCN TMPDEKSFFQ
     KVEATPYLKA ILNLNKEIKV TEGDLTEEPP REPAPPTTHL PVENRIILNE ALVEPLPDKH
     DREIYSNSTG FSNCIQTQDP YIQAFQHQQA KDETLFWATV EKRLAASTPK DNWTEFKTKR
     PLGDVLWLAY KRAMVLPDEP IKFNPELWWA CADEVQKTYL SKPIHALKNG ILRQSPDFDW
     NKLQIFLKSQ WVKKIDKIGK IDVNAGQTIA AFYQPTVMLF GTMARYMRRI RDTYQPGEIL
     INCEKNQKHI SKWVESNWNH RLPAYTNDFT AYDQSQDGAM LQFEVLKALH HDIPHEVVEA
     YVALKLNSKM FLGTLAIMRL TGEGPTFDAN TECNIAYTHA RFEIPKNVAQ MYAGDDCALN
     CRPVERQSFL PLVEKFTLKS KPKVFEQKVG SWPEFCGNLI TPRGYLKDPM KLQHCLQLAQ
     RKKPSEPGSL KDVAENYAMD LLPTYELGDA LYEIFDERQM NAHYQSVRTL ITCAHTKVLR
     VAQALQEDCT FFSSI