ID   RDRP_GGNNV              Reviewed;         982 AA.
AC   Q993M1;
DT   16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   29-SEP-2021, entry version 64.
DE   RecName: Full=RNA-directed RNA polymerase;
DE            Short=RdRp;
DE            EC=2.7.7.48;
DE   AltName: Full=RNA replicase;
DE            Short=Protein A;
OS   Greasy grouper nervous necrosis virus (GGNNV) (Epinephelus tauvina nervous
OS   necrosis virus).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes;
OC   Nodamuvirales; Nodaviridae; Betanodavirus.
OX   NCBI_TaxID=143921;
OH   NCBI_TaxID=203262; Epinephelus tauvina (Greasy grouper) (Perca tauvina).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Singapore;
RX   PubMed=11172107; DOI=10.1099/0022-1317-82-3-647;
RA   Tan C., Huang B., Chang S.F., Ngoh G.H., Munday B., Chen S.C., Kwang J.;
RT   "Determination of the complete nucleotide sequences of RNA1 and RNA2 from
RT   greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore
RT   strain.";
RL   J. Gen. Virol. 82:647-653(2001).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome composed of 2 RNA segments, RNA1 AND RNA2. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SIMILARITY: Belongs to the nodaviridae RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; AF319555; AAK21877.1; -; mRNA.
DR   Proteomes; UP000267218; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043647; Noda_Vmethyltr_dom.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR   Pfam; PF19222; Noda_Vmethyltr; 1.
DR   Pfam; PF00978; RdRP_2; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   2: Evidence at transcript level;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA-directed RNA polymerase;
KW   Transferase; Viral RNA replication.
FT   CHAIN           1..982
FT                   /note="RNA-directed RNA polymerase"
FT                   /id="PRO_0000222447"
FT   DOMAIN          579..701
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   REGION          879..982
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        880..901
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        906..943
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   982 AA;  110395 MW;  CC61FB47B75CE0DA CRC64;
     MRRFEFALAR MSGAAFCVYT GYRLLTSKWL ADRVEDYRQR IIAEKKQILR DAAMIRTQIQ
     REMELVRISV RKGHSHQEAA TERNSATETM LGVVEKCGYE PYVISPSPRE VGYHGSRQFY
     SLADFRQDYR RDDITDRHII VMTDVDYYVD MHELIGLGVP ILLYTFQPST VSGEVKDGYF
     TITDDSVHYR VAGGKDVRHR IWNYNHDTMY VCSRPRGFWA NLMQILRDIT GVTAICSFLY
     TKLGIAPFGD PVTMFTVDQF KMGEHRNIVS IVPFATCRSN LLKISEYGAE LEYMRYQQRN
     NIANFNAVTY ISENGPLISL GLEGNFASVQ LPLQDFENIR TAYELSKTNN LSDTVRRSGR
     PCKEAAIIHK CLQAECAVVS EVVHKPGDLA RHYQAVGSAY DTDPAEQGKC YAREYAPGPL
     TQTAVFPSES RSNELATIDG RIAGPQAKAK SREHITPKMR KVARDFVHHL VPIAGTGRPY
     PLTYVEEQQT KPLQRARNDA NRYHDEFTMM VKAFQKKEAY NAPNYPRNIS TVPHTQNVKL
     SSYTYAFKAS VLQHVPWYMP THTPAEIADA VQNLAASSTE LVETDYSKFD GTFLRFMREC
     VEFAIYKRWV HLDHLPELTT LLANEIQAPA VTRLGIKYDP DCSRLSGSAL TTDGNSIANA
     FVSYLAGRMA GMDDDEAWSW IGIVYGDDGL RSGNVSNELL TNTASSLGFD LKIVNRAPRG
     SPVTFLSRVY LDPWSSPASV QSPLRTLLKL HTTCDTQSEI DDIGWAKTQA YLVTDSKTPF
     IGHWCRAYQR NCTARVVQYA DYADIPFWVK NDDHVGNSWP QSESDDWNDI VANELGVTTA
     ELLKHLALLD AYAGPISGLP RLTTSIDLEP KMSVALDGEI QAGPSQNKTS KDGTNPTSDR
     SAPRRARAAL PGDDGHARRS RRSDRDPGKR DAHVRDKRPR RSSPPTRPVT PVPTPSSGDR
     GTDGDGLGRA AVRQRQRRRT QV