ID   RDRP_GRVMC              Reviewed;         839 AA.
AC   Q67683; A0A0K3AU46;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 2.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=RNA-directed RNA polymerase {ECO:0000255|PROSITE-ProRule:PRU00539};
DE            Short=RdRp;
DE            EC=2.7.7.48 {ECO:0000255|PROSITE-ProRule:PRU00539};
GN   ORFNames=ORF1-ORF2;
OS   Groundnut rosette virus (strain MC1) (GRV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus.
OX   NCBI_TaxID=1005060;
OH   NCBI_TaxID=43365; Clitoria.
OH   NCBI_TaxID=35936; Lablab purpureus (Hyacinth bean) (Dolichos lablab).
OH   NCBI_TaxID=3879; Medicago sativa (Alfalfa).
OH   NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
OH   NCBI_TaxID=3917; Vigna unguiculata (Cowpea).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA] (ISOFORM ORF1 PROTEIN), AND RIBOSOMAL
RP   FRAMESHIFT.
RX   PubMed=8811035; DOI=10.1099/0022-1317-77-9-2335;
RA   Taliansky M.E., Robinson D.J., Murant A.F.;
RT   "Complete nucleotide sequence and organization of the RNA genome of
RT   groundnut rosette umbravirus.";
RL   J. Gen. Virol. 77:2335-2345(1996).
CC   -!- FUNCTION: RNA-dependent RNA polymerase replicates the viral genome.
CC       {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC         Comment=Isoform RNA-directed RNA polymerase is produced by -1
CC         ribosomal frameshifting at the ORF1-ORF2 genes boundary. Isoform ORF1
CC         protein is produced by conventional translation.
CC         {ECO:0000269|PubMed:8811035};
CC       Name=RNA-directed RNA polymerase;
CC         IsoId=Q67683-1; Sequence=Displayed;
CC       Name=ORF1 protein;
CC         IsoId=Q67683-2; Sequence=VSP_041426;
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DR   EMBL; Z69910; CAA93799.1; -; Genomic_RNA.
DR   EMBL; Z69910; CTQ57207.1; -; Genomic_RNA.
DR   RefSeq; NP_619659.1; NC_003603.1.
DR   RefSeq; YP_009162058.1; NC_003603.1.
DR   GeneID; 1725261; -.
DR   GeneID; 940200; -.
DR   KEGG; vg:1725261; -.
DR   KEGG; vg:940200; -.
DR   Proteomes; UP000008927; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   Pfam; PF00998; RdRP_3; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   4: Predicted;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   Ribosomal frameshifting; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..839
FT                   /note="RNA-directed RNA polymerase"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5000147674"
FT   DOMAIN          504..619
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   VAR_SEQ         286..839
FT                   /note="Missing (in isoform ORF1 protein)"
FT                   /evidence="ECO:0000269|PubMed:8811035"
FT                   /id="VSP_041426"
SQ   SEQUENCE   839 AA;  93662 MW;  F500C9CF31FE7B63 CRC64;
     MATIRHIMDW LRPTFTPLAG VKSRQECIAH YGDDWALMIT QSRMTLSAEA QVNAWYEGGA
     EVNGSIPSVE GNAAAPQAAA VVAEPPHPSP AENEGEDASW VAALPAPPTY EVVQGRAPTL
     EEIHGASRLQ IVPYTGRARV IGEDEVLPSP VLSSIWRATR CPGRILRVLG TLLKPGACQR
     HLEDLREVQP QVCVGNPCEV KLQEEGAPMA YMNAQSIAME LRAMFGWQAA TPANRELGNR
     VARDILRDGC GATREQIWYM SSLALHMWFQ PTLCDLAIKA GAQNFLVGEV YARSGVETKT
     RPKILSPQIK VKLAARPRPV KRVSYNVDVL GPSADYGVHN NSLNNLVRGV NERVFYTNNQ
     GKLPLAPAAG AYQQIDCAAL KQFRVTPWSL DDVWMSYKGS QRVRYKQAVD SLGMRPLSKS
     DARVSTFIKA EKVNFRAKPD PAPRVIQPRD PRFNAVFAKY IKPLEPLLYK ALGKLYKYPC
     VAKGFNAVET GEIVAKKWKM FANPVCVGLD ASRFDQHVSV DALRFTHGVY RRFVKSSEFD
     KLLRWMYTNR CRGAAKDGFV KYTVNGCRMS GDMDTALGNC VLMVLMTRHL LMSLGIPHEL
     LDNGDDCIVI MDQEHLAKFN DAVKPYYSNL GFTMKVEEPV YSLERVDFCQ TRPVYDGKKW
     RMVRHITSVA KDCCTVINWE QLPSWLSAIG ECGIAVAGGI PVHNSFLRYL MRVGGTKGGI
     ENHLLWKNEG LSWYRMGMDL SHEKVVSDEA RLSFQTAFGI SPTMQRALED LYDGLGAPTV
     NGCDYRTVKT RDCREIECMP PRHYNHYFID CGMQPAGSQE QYVNPGYTIF EAAALWAQC