RDRP_GVAIS
ID RDRP_GVAIS Reviewed; 1707 AA.
AC Q67704;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=RNA replication protein;
DE Includes:
DE RecName: Full=RNA-directed RNA polymerase;
DE EC=2.7.7.48;
DE Includes:
DE RecName: Full=Helicase;
DE EC=3.6.4.13;
GN ORFNames=ORF1;
OS Grapevine virus A (isolate Is 151) (GVA).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus.
OX NCBI_TaxID=651358;
OH NCBI_TaxID=29760; Vitis vinifera (Grape).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-480.
RX PubMed=7944948; DOI=10.1007/bf01309473;
RA Minafra A., Saldarelli P., Grieco F., Martelli G.P.;
RT "Nucleotide sequence of the 3' terminal region of the RNA of two
RT filamentous grapevine viruses.";
RL Arch. Virol. 137:249-261(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA Minafra A.;
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA replication. The central part of this protein possibly
CC functions as an ATP-binding helicase (Probable). {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00805};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU00805};
CC -!- SIMILARITY: Belongs to the potexviruses/carlaviruses RNA replication
CC protein family. {ECO:0000305}.
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DR EMBL; X75433; CAA53182.1; -; Genomic_RNA.
DR PIR; S77910; S77910.
DR RefSeq; NP_619662.1; NC_003604.2.
DR GeneID; 940186; -.
DR KEGG; vg:940186; -.
DR Proteomes; UP000000679; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR Gene3D; 2.60.120.590; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR027450; AlkB-like.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
DR Pfam; PF13532; 2OG-FeII_Oxy_2; 1.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 3: Inferred from homology;
KW ATP-binding; Dioxygenase; Helicase; Hydrolase; Iron; Metal-binding;
KW Nucleotide-binding; Nucleotidyltransferase; Oxidoreductase;
KW Reference proteome; RNA-directed RNA polymerase; Transferase;
KW Viral RNA replication.
FT CHAIN 1..1707
FT /note="RNA replication protein"
FT /id="PRO_0000401089"
FT DOMAIN 66..242
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01079"
FT DOMAIN 645..738
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT DOMAIN 892..1051
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 1052..1182
FT /note="(+)RNA virus helicase C-terminal"
FT DOMAIN 1469..1580
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT BINDING 663
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 665
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 720
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 729
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 1707 AA; 195502 MW; 70494B3CCF312DE4 CRC64;
MSISVSSQRV AVSNLYTNGS EESVKAIKEL KSKRLLETET RLDGLFDYYI PDTLREILTG
YGMEFSVHSF QGHAHPVSKM IENHMLYRVA PNYFSSNTLV VSCKESKIKR LRLKNANNRN
LNFTQYNRLV HANHHHRYEN AFRELDVGNL TNLINKEDQS ECIFIHDEVQ YWSLDEMQRF
LGSLSKVDRV VYSIIYPSEV EAGYSQSLFP EAYTFDLKDR RLVWYPDGKA EGAYTQPVNP
WLLRCSKTED SKGRSWTITK LQTIGAHHLF SAIKGSYLTE ESYKYDNFTI INPNDVLKGK
RGGKPLYLRA RMIKPTLLYL LALKKSDSNS AVAKLRMLSS REENMDEALF VAQLAKQIKD
TALYDKMGNP NLRSILSESF YDIAGSLFTR LFNRPEYDAR CLEKFIRSCE TTEIHVERRY
MEGIRRGASF KVQNVMDWVE DDSANALSEV NFLDISWNDR VSEPYGIECI HGEGSRIRVP
LSRILRAHEL IAGVQTDVEI NFPRYVCSSR ALIHFRQYLI KLGRFSFMES RAIKDIEDIQ
AGLEEGVITE EEAELRLLPT TKPKITEIHM DDDTPGTSGE SDVEKFKSVR SLCREEIYSE
KLKGREVAFY SRHSKEYKYN GGSHRSLGWD EALNELTQEL GLDDSYDHCL IQRYTAGGSI
GFHADDEPCY LPGGSVVTVN LHGDATFEVK ENQSGKIEKK ELHDGDVYVM GPGMQQTHKH
RVTSHTDGRC SITLRNKTVD YEARKGDEDS EYEEDKAELD EGIDYLQKNQ GNMCSLKAFA
DHMQLSTPSV IAIVNGASPQ TLREIEDGGY SLATLVNLSK ALDFPIAIHG ERGYAETPGS
YRRLHLKITS GHVEPFEGVT SKGGFREAML LGDGVGVGHF RVDKAKADRL AQSFYNGNTG
VLLGKYNKGK MHTGEIEEPK EVLTAFGFAG SGKSHWCQTI LKHCSVEKVL VISPRKVLRD
DWVAKISKKH RVVTFEVAFM DDYGCKDIVI DEIGLLPPGY IDLVIAAHQP RTLVLLGDPL
QSTYHSKRDN VVLEASQEDV FNRVRGKLPY LCYSHRLPRN CKLFEIECMG AESEKRVVYR
SNRLKDEPTI CATRAMKEEK GSGWYTVSET QGLSFKSCLI YLDEHWAKKE DEDVMVALTR
SRGEIGIHVT PALKKKLITN AKSTLLKKVL KGETYRRSEI VAMVRKHIPE TTVLFEESRL
AETVDYEARL AGDPYLKSLL ALYDEIEMED IEIEEPVTLE PTKTHLALST KMNELAPFDL
KAKEHREQHT EAGRTEQIDE NGYQGEVGDP MTHKALYLRH TSDDTATFMM SVKKRLRFRN
YEANRRKYKT CHGIGHQMFS VFKDTYQLKE IDSLPELERC EMEFMKKRIE KSTGLIEKHA
GRSDPDWPSN YLKIFLKQQT CTKMEKRGVD AKAGQTIACF AHSVLCRFGP ILRQTEKALR
ELLPEKLMIY SQKKYMDLDK WAKTWVESMM GTDSDYEAFD RSQDEKVLDL EVEVLRFFLW
PEDLIREYEE LKLMMGCALG DLAVMRFSGE FGTFFFNTVC NMVFSCMRYH IDRNTPMCFA
GDDMYSPGIL RVKKDYEATL DQLTLKAKVH ISEEPLFCGW RMSPFGIIKE PNLILDRWKI
ALRSGNLSLC LVNYAIEASF GYRLSEHLYD VNIDVDAQQE LVREIVIKKH LLPKKISDLF
SEDECERHSD GDEDFLSNDV ARLYRIE
