ID   RDRP_HV19S              Reviewed;         835 AA.
AC   O57044;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 2.
DT   29-SEP-2021, entry version 60.
DE   RecName: Full=Probable RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
OS   Helminthosporium victoriae virus-190S (Hv190SV).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes;
OC   Ghabrivirales; Totiviridae; Victorivirus.
OX   NCBI_TaxID=45237;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8901618; DOI=10.1073/pnas.93.22.12541;
RA   Huang S., Ghabrial S.A.;
RT   "Organization and expression of the double-stranded RNA genome of
RT   Helminthosporium victoriae 190S virus, a totivirus infecting a plant
RT   pathogenic filamentous fungus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:12541-12546(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Ghbarial S.A., Nibert M., Xu L.L., Talkington M.W.T.;
RT   "Victorivirus: a new genus in the family Totiviridae.";
RL   Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   EXPRESSION.
RX   PubMed=10623763; DOI=10.1128/jvi.74.2.997-1003.2000;
RA   Soldevila A.I., Ghabrial S.A.;
RT   "Expression of the Totivirus Helminthosporium victoriae 190S virus RNA-
RT   dependent RNA polymerase from its downstream open reading frame in
RT   dicistronic constructs.";
RL   J. Virol. 74:997-1003(2000).
CC   -!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
CC       genome. Catalyzes the transcription of fully conservative plus-strand
CC       genomic RNAs that are extruded from the virion into the cytoplasm where
CC       they function as mRNAs for translation of viral proteins and also as
CC       substrates for encapsidation to form new virions. Once encapsidated,
CC       the positive strand is converted to dsRNA by the RNA-directed RNA
CC       polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48;
CC   -!- MISCELLANEOUS: RDRP is released as a separate protein consequent to an
CC       apparently coupled termination-reinitiation mechanism.
CC   -!- SIMILARITY: Belongs to the totiviridae RNA-directed RNA polymerase
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U41345; AAB94791.2; -; Genomic_RNA.
DR   RefSeq; NP_619670.2; NC_003607.2.
DR   PRIDE; O57044; -.
DR   GeneID; 940192; -.
DR   KEGG; vg:940192; -.
DR   Proteomes; UP000000352; Genome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR001795; RNA-dir_pol_luteovirus.
DR   Pfam; PF02123; RdRP_4; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-binding; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..835
FT                   /note="Probable RNA-directed RNA polymerase"
FT                   /id="PRO_0000404512"
SQ   SEQUENCE   835 AA;  91320 MW;  5219337910812FB4 CRC64;
     MSDPQERSKA YGLLGERLYA VASANSHMLA GYDSLDFTAR LVRLTGEATA LKAVDPLLPC
     AVSLLFMDFP LQLPCTPEET LRLVRRAYDP NTLEEVDYST LSGYATQFAR VKGQRGRWRH
     LGHLVCNDKA FRERYFPKKK HAAAAIKTNI RLGPLARAWA ARYGLAALGS HLAYMVGMPN
     DRACATLLLA QTYKARFGSE GVAWAIASVR QPENAKGLSN ALKALGSNTS EPGALFVEAN
     TLQGRYDRTL DMDHEVESRC SPAAIADQVI PYTDELGACI DFILDTELGG DTIELPDEDE
     WWTSRWLWCV NGSQNALSDK ALGIKNKSGQ RYRRMAAEEV NNNPVPAWNG HTSVSPSVKL
     ENGKDRAIFA CDTRSYFAFT YWLTPIEKKW RGARVILNPG EGGLYGTARR IRGSQTSGGV
     NLMLDYDNFN SQHSNETMAA LYEKALSRTN APAYLKKAVA ASVESTYIHY KGRDRHVLGT
     LMSGHRATTF TNSVLNAAYI CYAVGIPAFK RMISLHAGDD VYLRLPTLAD CATTLNNTKR
     VGCRMNPTKQ SIGYTGAEFL RLGINKSYAI GYLCRAIASL VSGSWTSLDE LQPLNALNGA
     IVQTRSCLNR GAATGLPELI SASFVGLRGF KRRDLLELLT GVATIKPGPV YTSSCVIREY
     VVEQPPPPQF DVPPGAGMHA TMSYLARHTT LVEAQALEIA RPAIKSLMLS SSYGKAGPGA
     QTRPHVPMPK LRRQPPRVAV GFSMAHELTG RGVKEGCLSG HPLLRLFEQR LTDDDLRALV
     ALVGGNTSAK DIRAEAFGAE SSSSTIMGIL PHSDASNYCK RTRNGNIIVP YHIRS